β-Glucuronidase
- Product Name
- β-Glucuronidase
- CAS No.
- 9001-45-0
- Chemical Name
- β-Glucuronidase
- Synonyms
- B-GLUCURONIDASE;BETA-GLUCURONIDASE;KETODASE;glucurase;EC 3.2.1.31;EC: 3.2.1.31;β-Glucuronidas;beta-glucuronidas;beta-d-glucuronidase;β-Glucuronidase–Agarose.
- CBNumber
- CB5318344
- Molecular Formula
- NULL
- Formula Weight
- 0
- MOL File
- Mol file
β-Glucuronidase Property
- storage temp.
- -20°C
- form
- powder
- color
- yellow-brown
- biological source
- limpet (Patella vulgata)
- Water Solubility
- water: soluble
- Merck
- 13,4480
- Specific Activity
- 5,000,000-20,000,000units/g protein, pH 6.8 (30 min assay)
Safety
- Hazard Codes
- Xn
- Risk Statements
- 42/43
- Safety Statements
- 22-36/37-45-23
- WGK Germany
- 3
- RTECS
- LZ8990000
- F
- 3-10
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H317May cause an allergic skin reaction
H318Causes serious eye damage
H334May cause allergy or asthma symptoms or breathing difficulties if inhaled
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P272Contaminated work clothing should not be allowed out of the workplace.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- SRE0095
- Product name
- Fast β-Glucuronidase, Recombinant
- Purity
- from limpets (Patella Vulgata), aqueous solution
- Packaging
- 10ML
- Price
- $444
- Updated
- 2025/07/31
- Product number
- SRE0095
- Product name
- Fast β-Glucuronidase, Recombinant
- Purity
- from limpets (Patella Vulgata), aqueous solution
- Packaging
- 25ML
- Price
- $943.35
- Updated
- 2025/07/31
- Product number
- SRE0095
- Product name
- Fast β-Glucuronidase, Recombinant
- Purity
- from limpets (Patella Vulgata), aqueous solution
- Packaging
- 50ML
- Price
- $2010
- Updated
- 2025/07/31
- Product number
- SRE0037
- Product name
- β-Glucuronidase from abalone
- Purity
- aqueous solution, β-glucuronidase ≥100,000?units/mL
- Packaging
- 10ml
- Price
- $188
- Updated
- 2025/07/31
- Product number
- SRE0037
- Product name
- β-Glucuronidase from abalone
- Purity
- aqueous solution, β-glucuronidase ≥100,000?units/mL
- Packaging
- 25mL
- Price
- $427
- Updated
- 2025/07/31
β-Glucuronidase Chemical Properties,Usage,Production
Chemical Properties
Off-white or yellow powder, easily soluble in water, pI5.1, optimum pH 4.5-5.0.Stability: under the condition of 4 ℃, keep for six months with 10% decrease in vitality; keep for twelve months with 20% decrease in vitality; adding butanol can increase its stability; if there is turbidity, it does not affect the activity. Inhibitors are D-glucuronic acid, D-galacturonic acid and organic peroxides. 1% aqueous solution has an absorption coefficient of 17.0 at 280 nm. it can rapidly hydrolyze steroidal glucosinolates and other glucosinolates, but not α- or β-glucosides. enzyme reaction: β-D-glucuronic acid + H2O ═D-glucuronic acid + alcohol bound.
Uses
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Uses
β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
Uses
In the determination of urinary steroids and of steroid conjugates in blood.
General Description
β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.
Biochem/physiol Actions
β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.
β-Glucuronidase Preparation Products And Raw materials
Raw materials
Preparation Products
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