β-Glucuronidase

β-Glucuronidase Property

storage temp. 

-20°C

form 

powder

color 

yellow-brown

biological source

limpet (Patella vulgata)

Water Solubility 

water: soluble

Merck 

13,4480

Specific Activity

5,000,000-20,000,000units/g protein, pH 6.8 (30 min assay)

Safety

Hazard Codes 

Xn

Risk Statements 

42/43

Safety Statements 

22-36/37-45-23

WGK Germany 

3

RTECS 

LZ8990000

F 

3-10

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H317May cause an allergic skin reaction

H318Causes serious eye damage

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

β-Glucuronidase Chemical Properties,Usage,Production

Chemical Properties

Off-white or yellow powder, easily soluble in water, pI5.1, optimum pH 4.5-5.0.Stability: under the condition of 4 ℃, keep for six months with 10% decrease in vitality; keep for twelve months with 20% decrease in vitality; adding butanol can increase its stability; if there is turbidity, it does not affect the activity. Inhibitors are D-glucuronic acid, D-galacturonic acid and organic peroxides. 1% aqueous solution has an absorption coefficient of 17.0 at 280 nm. it can rapidly hydrolyze steroidal glucosinolates and other glucosinolates, but not α- or β-glucosides. enzyme reaction: β-D-glucuronic acid + H2O ═D-glucuronic acid + alcohol bound.

Uses

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Uses

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.

Uses

In the determination of urinary steroids and of steroid conjugates in blood.

General Description

β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.

Biochem/physiol Actions

β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.