Overview Applications Mode of action Application methods Risk and Warning References
ChemicalBook > CAS DataBase List > Daminozide

Daminozide

Overview Applications Mode of action Application methods Risk and Warning References
Product Name
Daminozide
CAS No.
1596-84-5
Chemical Name
Daminozide
Synonyms
Ferrostatin-1;B9;ALAR;DAMINOZID;SADH;b995;dyak;NINE;B 995;B-995
CBNumber
CB5752603
Molecular Formula
C6H12N2O3
Formula Weight
160.17
MOL File
1596-84-5.mol
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Daminozide Property

Melting point:
162-164 °C(lit.)
Boiling point:
286.06°C (rough estimate)
Density 
1.2751 (rough estimate)
refractive index 
1.4500 (estimate)
storage temp. 
Keep in dark place,Inert atmosphere,Store in freezer, under -20°C
solubility 
Soluble in DMSO (up to 50 mg/ml), or in Water (up to 20 mg/ml with warming).
pka
5.59±0.10(Predicted)
form 
White powder
color 
White crystalline
Water Solubility 
100 g/L
Merck 
14,2810
BRN 
1863230
Stability:
Stable. Combustible. Incompatible with strong oxidizing agents, strong bases, strong acids, wetting agents, oils, copper-containing compounds.
InChIKey
NOQGZXFMHARMLW-UHFFFAOYSA-N
CAS DataBase Reference
1596-84-5(CAS DataBase Reference)
NIST Chemistry Reference
Butanedioic acid, mono(2,2-dimethylhydrazide)(1596-84-5)
EPA Substance Registry System
Daminozide (1596-84-5)
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Safety

Hazard Codes 
Xn
Risk Statements 
40
Safety Statements 
36/37-24/25
RIDADR 
UN2811 (toxic solid, organic, n.o.s.)
WGK Germany 
2
RTECS 
WM9625000
HS Code 
29280000
Hazardous Substances Data
1596-84-5(Hazardous Substances Data)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
45418
Product name
Daminozide
Purity
PESTANAL
Packaging
250mg
Price
$28.2
Updated
2024/03/01
Sigma-Aldrich
Product number
251635
Product name
Daminozide - CAS 1596-84-5 - Calbiochem
Packaging
1g
Price
$76.5
Updated
2022/05/15
TCI Chemical
Product number
D4015
Product name
Daminozide
Purity
>98.0%(GC)(T)
Packaging
5g
Price
$66
Updated
2024/03/01
TCI Chemical
Product number
D4015
Product name
Daminozide
Purity
>98.0%(GC)(T)
Packaging
25g
Price
$237
Updated
2024/03/01
Cayman Chemical
Product number
12033
Product name
Daminozide
Purity
≥95%
Packaging
5g
Price
$22
Updated
2023/06/20
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Daminozide Chemical Properties,Usage,Production

Overview

Daminozide is one of the first plant growth retardants[PGRs] registered for use on agricultural crops. It was first registered in 1963 for use on potted chrysanthemums in the US and was later approved for use on food crops[1-2]. Although it is still allowed for used on ornamentals plants, its use on food crops was withdrawn in 1990 by the United States environmental protection agency[USEPA] due to its possible health risks such as cancer inducing risks[1-2]. Two major commercial products available in the United States and Canada that contain daminozide: B-Nine[from OHP] and Dazide[from Fine Americas]. Both come as a water-soluble granule containing 85 percent of the active ingredient[3-4]. Based on research conducted by Jim Barrett at the University of Florida, these products are only effective as a foliar spray; if applied as a drench, the chemical is rapidly broken down by the growing media. A typical application spray volume for daminozide is the same as that for other PGRs: 2 quarts per 100 square feet of growing area. B-Nine and Dazide are water-soluble PGRs that permeate leaf surfaces relatively slowly. Movement into leaf tissue only occurs while the leaf surface is still wet with the PGR solution and therefore, the absorption and activity of daminozide increases under slow-drying conditions. Compared with other PGR products, daminozide has a fairly short residual effect, typically lasting one to two weeks but sometimes up to four weeks depending on crop and rate[3-6].

Applications

As a plant growth retardant, daminozide has various applications as dwarfing agent, fruit-setting agent, rooting agent and preservative agent[7]. After treatment, plants can absorb, transport and distribute to various parts of the plant. The initial effect of B9 is to inhibit the synthesis of auxin, inhibit the transport of auxin in plants and the biosynthesis of gibberellin. It can also delay the senescence of leaf lettuce, inhibit the decay and discoloration of mushrooms, and has less effect on green cauliflower and stone cypress. In addition, daminozide preserves chlorophyll in plants and prolongs the life of some perishable vegetables[7].

Mode of action

Daminozide can retard shoot growth in certain plant species, sharing similar effect with the prohexadione, which is an acylcyclohexanedione. This inhibition has been shown to be a result of competition with the natural co‐substrate, 2-oxoglutarate, at the active site of hydroxylases involved in the later stages of the gibberellin[GA] biosynthesis pathway. Detailed analysis found that daminozide inhibits only the bean 3β‐hydroxylase to a significant degree, whereas prohexadione inhibited both the bean and pumpkin enzymes. However, in general, this two plant growth regulator has the same mode of action as prohexadione in distinct plant species, namely to inhibit the 3β-hydroxylase and, to a lesser extent, the 2β-hydroxylase, further resulting in the suppression of the late stages of gibberellin metabolism[8].
The effects of daminozide on plants include:

  1. Delay plant vegetative growth, make the leaves thick green, small and thick, the plant compact and strong, the root system developed, increasing the dry weight of the roots to reduce the proportion of crown roots, which is conducive to control excessive growth and flower bud differentiation[9].
  2. Increase chlorophyll content of crops, delay the senescence of chloroplasts, slow down the growth rate, and have a high photosynthetic net assimilation rate, which is conducive to increasing dry matter accumulation, improving fruit quality, hardness and fruit set rate, and promoting fruit ripening[9].
  3. Increase the sugar content of plant cells, reduce energy consumption, reduce transpiration, which is beneficial to reduce physiological diseases[10].
  4. Promote the biosynthesis of anthocyanins, which is beneficial to improve the color of the fruit and prevent the fruit from decolorizing during storage. Daminozide can be quickly decomposed by microorganisms in the soil[11].

Application methods

Daminozide has a fairly wide range of efficacy on ornamentals and is commonly used on young plants such as seedling plugs and liners. It cannot be legally applied to herbs or other food crops. Typical spray rates for young plants range from 1,500 to 2,500 ppm, whereas higher rates of up to 5,000 ppm are sometimes used on finish plants[12,13]. Carefully follow the mixing instructions on the product labels to deliver the desired concentration. Applications are best made just as plants begin to rapidly elongate. Spray applications are more effective when made at a high humidity, on a cloudy day, at low temperatures and when the air is calm. A spray made during the heat of the day can dry rapidly, resulting in reduced effectiveness[13-15]. Applications should be made to well-watered plants with dry leaves. Wilted plants can absorb less PGR, and wet leaves can dilute the PGR concentration. Overhead irrigation should not be made soon after an application, as the water can wash off the PGR before it has been allowed to enter leaves. Once daminozide enters leaf tissue however, it quickly moves throughout the plant to inhibit subsequent extension growth of stems and leaves[13-15].

Risk and Warning

Based on the investigation of the United States environmental protection agency[USEPA], Daminozide has to potential to induce cancer[16,17]. It is also known that it is a selective inhibitor of human KDM2/7 histone demethylases[18].

References

  1. United States Environmental Protection Agency, "Daminozide[Alar] Pesticide Canceled for Food Uses" Archived October 3, 2012, at the Wayback Machine.[press release], 7 November 1989
  2. United States Environmental Protection Agency, Office of Prevention, Pesticides And Toxic Substances[September 1993]. "R.E.D. Facts: Daminozide"
  3. https://www.extension.purdue.edu/extmedia/ho/ho-248-w.pdf
  4. https://www.purdue.edu/hla/sites/cea/wp-content/uploads/sites/15/2008/10/Comparing-PGR-Final.pdf
  5. https://www.ballpublishing.com/pdf/PGR_GUIDE_2013-LowRez.pdf
  6. https://www.maximumyield.com/definition/3304/daminozide
  7. http://www.lmdchinapgr.com/news/116.html
  8. https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1399-3054.1997.tb01001.x
  9. https://www.degruyter.com/downloadpdf/j/fhort.2017.29.issue-1/fhort-2017-0004/fhort-2017-0004.xml
  10. https://www.ncbi.nlm.nih.gov/pubmed/25342260
  11. https://link.springer.com/article/10.1007/s00344-012-9315-3
  12. https://www.panamseed.com/utility/CultureSheetPDF.aspx?pagename=culture.aspx&txtPHID=046706198039762&type=Ann
  13. https://www.syngentaflowers-us.com/file/4336/download?token=UMCcNgWb
  14. https://www.ballpublishing.com/pdf/PGR_GUIDE_2013-LowRez.pdf
  15. http://journal.ashspublications.org/content/125/2/195.full.pdf
  16. https://www.ncbi.nlm.nih.gov/pubmed/22724510
  17. https://phytotechlab.com/mwdownloads/download/link/id/2216/
  18. 18. Rose, Nathan R., et al. "Plant Growth Regulator Daminozide Is a Selective Inhibitor of Human KDM2/7 Histone Demethylases." Journal of Medicinal Chemistry 55.14(2012]:6639-6643.

Description

Alar is a systemic growth regulator approved in the United States for use on ornamental plants such as chrysanthemums, poinsettias, and bedding plants located in enclosed structures such as greenhouses. Alar reduces internode elongation; induces heat, drought, and frost resistance; and produces darker foliage and stronger stems as well as earlier and multiple flowers and fruits. A spray is often applied at the rate of 1500–10 000 ppm.

Chemical Properties

Daminozide is a colorless crystalline solid.

Chemical Properties

white crystalline powder

Uses

Plant cell culture, tested.

Uses

Alar is a systemic agent (i.e., taken up by the fruit) and cannot be removed by washing or peeling.

Uses

Daminozide is a plant growth regulator used to improve crop quality and maximize yield.Used as a standard for Pesticide detection. Useful in cannabis testing kits as a component of pesticide mixes (P698240).

Uses

Plant growth regulator.

Definition

ChEBI: Daminozide is a straight-chain fatty acid.

General Description

Odorless white crystals or powder.

Air & Water Reactions

Slightly soluble in water.

Reactivity Profile

Daminozide may be heat sensitive. Incompatible with strong oxidizing agents, strong acids, and bases. Also incompatible with wetting agents, alkaline materials, oils and copper-containing compounds. May corrode metals . May generate toxic gases with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. May generate flammable gases with alkali metals. Explosive reactions can occur with strong oxidizing agents, metal salts, peroxides, and sulfides.

Fire Hazard

Flash point data for Daminozide are not available; however, Daminozide is probably combustible.

Agricultural Uses

Plant growth regulator: Daminozide is a systemic growth regulator registered for use on ornamentals, including potted chrysanthemums and poinsettias, and bedding plants in enclosed structures. U.S. sales for food and feed crops were halted in 1989 because of health considerations, i. e., the Alar scare on apples.

Trade name

ALAR®; ALAR-85®; AMINOZID®; AMINOZIDE®; B-9®; B-995®; B-NINE®; DAZIDE®; DAZIDE®; ENHANCE®; DIMAS®; KYLAR®; SADH®

Safety Profile

Suspected carcinogen with experimental carcinogenic and tumorigenic data. Moderately toxic by ingestion and intraperitoneal routes. When heated to decomposition it emits toxic fumes of NOx

Potential Exposure

Daminozide is a herbicide/plant growth regulator used on certain fruit (especially apples) to improve the balance between growth and fruit production and to improve fruit quality and synchronize maturity. A RUP. United States sales were suspended in 1989 due to health considerations.

First aid

Skin Contact: Flood all areas of body that havecontacted the substance with water. Do not wait to removecontaminated clothing; do it under the water stream. Use soapto help assure removal. Isolate contaminated clothing whenremoved to prevent contact by others. Eye Contact: Removeany contact lenses at once. Flush eyes well with copious quantities of water or normal saline for at least 2030 min. Seekmedical attention. Inhalation: Leave contaminated area immediately; breathe fresh air. Proper respiratory protection must besupplied to any rescuers. If coughing, difficult breathing, orany other symptoms develop, seek medical attention at once,even if symptoms develop many hours after exposure.Ingestion: If convulsions are not present, give a glass or two ofwater or milk to dilute the substance. Assure that the person’sairway is unobstructed and contact a hospital or poison centerimmediately for advice on whether or not to induce vomiting.

Metabolic pathway

Daminozide is oxidized by photochemically generated singlet oxygen, with rose bengal as a sensitizing agent in methanol-d4 to yield equimolar amounts of N,N- dimethylnitrosamine (DMN) and succinic anhydride as the only products detected by 1H and 13C NMR. The reaction is efficiently inhibited by 2,5-dimethylfuran as a competitor for, or sodium azide as a quencher of, singlet oxygen. Humic acid, similar to that found in natural and waste waters, and a red pigment isolated from apple peel also sensitize the photodegradation of daminozide to produce DMN and succinic anhydride.

storage

Room temperature

Shipping

UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.

Toxicity evaluation

Alar does not degrade following contact with water but degrades rapidly in soil resulting in volatile compounds (including formaldehyde) and bound residues; therefore mobility is not considered a concern. In greenhouse studies, alar persistence ranged from 3 to 4 days in different soils. Since alar is registered for greenhouse use only, agricultural runoff into groundwater is not expected to be a concern.

Incompatibilities

Daminozide may be heat sensitive. Incompatible with strong oxidizing agents, strong acids, and bases. Also incompatible with wetting agents, alkaline materials, oils and copper-containing compounds. May corrode metals (NTP, 1992). May generate toxic gases with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and reducing agents. May generate flammable gases with alkali metals. Explosive reactions can occur with strong oxidizing agents, metal salts, peroxides, and sulfides . Compounds of the carboxyl group react with all bases, both inorganic and organic (i.e., amines) releasing substantial heat, water and a salt that may be harmful. Incompatible with arsenic compounds (releases hydrogen cyanide gas), diazo compounds, dithiocarbamates, isocyanates, mercaptans, nitrides, and sulfides (releasing heat, toxic and possibly flammable gases), thiosulfates and dithio- nites (releasing hydrogen sulfate and oxides of sulfur).

References

1) Rose et al. (2012), Plant growth regulator daminozide is a selective inhibitor of human KDM2/7 histone methylases; J. Med. Chem., 55 6639 2) Chen et al. (2016),?Lysine demethylase 2A promotes stemness and angiogenesis of breast cancer by upregulating Jagged1; Oncotarget, 7 27689

Daminozide Preparation Products And Raw materials

Raw materials

1,1-Dimethylhydrazine Succinic anhydride Succinic acid

Preparation Products

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Daminozide Suppliers

Americas 1 Belgium 1 Canada 2 China 284 Europe 6 France 2 Germany 4 India 28 Japan 4 Poland 1 Slovakia 1 Switzerland 2 United Kingdom 11 United States 40 Global 387
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Shandong Xiya Chemical Co., Ltd.
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4009903999 13395398332
Fax
0539-6365991
Email
sales@xiyashiji.com
Country
China
ProdList
20806
Advantage
60
Raw material medicin reagent co.,Ltd
Tel
Email
sales@njromanme.com
Country
China
ProdList
4529
Advantage
58
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View Lastest Price from Daminozide manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Daminozide 1596-84-5
Price
US $0.00/KG
Min. Order
1KG
Purity
98.0%
Supply Ability
1000kg/month
Release date
2023-01-04
Hebei Weibang Biotechnology Co., Ltd
Product
Daminozide 1596-84-5
Price
US $10.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10 mt
Release date
2024-11-13
Henan Bao Enluo International TradeCo.，LTD
Product
Daminozide 1596-84-5
Price
US $50.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
1000tons
Release date
2023-08-07

1596-84-5, DaminozideRelated Search:

Succinic acid Succinic anhydride Formylhydrazine DL-α-Tocopherol 4-(Diethylamino)salicylaldehyde Folic acid Carbohydrazide Tebufenozide N-Methylolacrylamide Butanedihydrazide Dimethylsulfamoyl chloride 1,5-Diphenylcarbazide Adipic dihydrazide 5-Chlorovaleric acid POLY(N-ISOPROPYL ACRYLAMIDE) 4-Dimethylaminobenzaldehyde Diphenolic acid Azodicarbonamide

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  • NINE