α-Glycerophosphate dehydrogenase

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α-Glycerophosphate dehydrogenase

Product Name: α-Glycerophosphate dehydrogenase
CAS No.: 9075-65-4
Chemical Name: α-Glycerophosphate dehydrogenase
Synonyms: GDH;ALPHA-GDH;EC 1.1.1.8;dehydrogenase,glycerolphosphate;A-GLYCEROPHOSPHATE DEHYDROGENASE;Α-GLYCEROPHOSPHATE DEHYDROGENASE;GLYCEROL-3-PHOSPHATE DEHYDROGENASE;Glycerol-3-PhosphateDehydrogenaseEx;SN-GLYCERO-3-PHOSPHATE DEHYDROGENASE;ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE
CBNumber: CB6222707
Molecular Formula: NULL
Formula Weight: 0
MOL File: Mol file

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α-Glycerophosphate dehydrogenase Property

storage temp.: -20°C
form: suspension
color: white
biological source: rabbit muscle
Specific Activity: 100-300units/mg protein
EPA Substance Registry System: Dehydrogenase, glycerol phosphate (9075-65-4)

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Safety

WGK Germany: 3
F: 3-10

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: G6751
Product name: α-Glycerophosphate Dehydrogenase from rabbit muscle
Purity: Type I, ammonium sulfate suspension, 100-300?units/mg protein
Packaging: 200units
Price: $65.8
Updated: 2024/03/01

Sigma-Aldrich

Product number: G6880
Product name: α-Glycerophosphate Dehydrogenase from rabbit muscle
Purity: Type X, lyophilized powder, ≥100?units/mg protein
Packaging: 100units
Price: $87.5
Updated: 2024/03/01

Sigma-Aldrich

Product number: G6751
Product name: α-Glycerophosphate Dehydrogenase from rabbit muscle
Purity: Type I, ammonium sulfate suspension, 100-300?units/mg protein
Packaging: 1000units
Price: $126
Updated: 2024/03/01

Sigma-Aldrich

Product number: G6880
Product name: α-Glycerophosphate Dehydrogenase from rabbit muscle
Purity: Type X, lyophilized powder, ≥100?units/mg protein
Packaging: 500units
Price: $316
Updated: 2024/03/01

Sigma-Aldrich

Product number: G6751
Product name: α-Glycerophosphate Dehydrogenase from rabbit muscle
Purity: Type I, ammonium sulfate suspension, 100-300?units/mg protein
Packaging: 5000units
Price: $460
Updated: 2024/03/01

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α-Glycerophosphate dehydrogenase Chemical Properties,Usage,Production

Uses

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:

in the reaction mixture to measure the glycerol kinase activity
to demonstrate compartmentalized enzymatic reactions where NADH is involved
in the reaction mixture assay for L-fuculose-1-phosphate aldolase

General Description

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

Purification Methods

The dehydrogenase is recrystallised by adding (NH4)2SO4 to 0.45 saturation at pH 5.5 at 4oC, and the small amount of precipitate is removed, then a saturated solution of (NH4)2SO4 is added dropwise from time to time over several days in the cold room. The crystals are collected and recrystallised until they have maximum activity. The enzyme is stable in half-saturated (NH4)2SO4 for several weeks at 4o. The equilibrium [dihydroxyacetone][NADH][H+]/[G-3-P][NAD] is 1.0 x 10 -12M in Tris buffer at 25o. It uses NAD ten times more efficiently than NADP. The Km for G-3-P is 1.1 x 10 -4M, for NAD it is 3.8 x 10-4M and for dihydroxyacetone it is 4.6 x 10-4M in phosphate buffer pH 7.0 and at 23.3o. Dihydroxyacetone phosphate and fructose-1,6-diphosphate are inhibitors. [Branowski J Biol Chem 180 515 1949, The Enzymes 7 85 1963, Young & Pace Arch Biochem Biophys 75 125 1958, Walsh & Sallach Biochemistry 4 1076 1965.]

α-Glycerophosphate dehydrogenase Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from α-Glycerophosphate dehydrogenase manufacturers

Career Henan Chemical Co

Product: Glycerol-3-phosphate dehydrogenase 9075-65-4
Price: US $1.00/kg
Min. Order: 1kg
Purity: 95%-99%
Supply Ability: 1000kg
Release date: 2018-12-25

9075-65-4, α-Glycerophosphate dehydrogenaseRelated Search:

SALCOMINE Tosylmethyl isocyanide 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE PHENYLSELENOL METHYL ISOCYANOACETATE Ethyl isocyanoacetate COBALT(II) ACETYLACETONATE TERT-BUTYL ISOCYANIDE Tris(2,4-pentanedionato)chroMiuM(III) N-BUTYLISOCYANIDE Cupric acetylacetonate 3-PHOSPHOGLYCERATE DEHYDROGENASE*FROM CH ICKEN LIVER Ferric acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) 2,4-PENTANEDIONE, SILVER DERIVATIVE Benzyl isocyanide TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Aluminum acetylacetonate

EC 1.1.1.8

GDH

GLYCEROL-3-PHOSPHATE DEHYDROGENASE

ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE TYPE I

ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE TYPE X

ALPHA-GDH

ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE

A-GLYCEROPHOSPHATE DEHYDROGENASE

SN-GLYCERO-3-PHOSPHATE DEHYDROGENASE

SN-GLYCEROL-3-PHOSPHATE: NAD 2-OXIDOREDUCTASE

SN-GLYCEROL-3-PHOSPHATE:NAD+ 2-OXIDOREDUCTASE

SN-GLYCEROL-3-PHOSPHATE:NAD+ 2-OXIDOREDUCTASE TYPE I

SN-GLYCEROL-3-PHOSPHATE:NAD+ 2-OXIDOREDUCTASE TYPE X

Α-GLYCEROPHOSPHATE DEHYDROGENASE

dehydrogenase,glycerolphosphate

α-Glycerophosphate Dehydrogenase from rabbit muscle,sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase

alpha-GDH, Glycerol-3-phosphate dehydrogenase (NAD+), sn-Glycerol-3-phosphate: NAD+ 2-oxidoreductase

alpha-Glycerophosphate dehydrogenase (from Rabbit muscle)

A-glycerophosphate dehydrogenase type I from rabb

glycerol-3-phosphate dehydrogenase from rabbit musc~270U/mg

sn-Glycero-3-phosphate Dehydrogenase rabbit muscle

A-GLYCEROPHOSPHATE DEHYDROGENASE TYPE X FROM RABBIT

GLYCERIN-3-PHOSPHAT-DEHYDROGENASE AUS KANI NCHENMUSK.120U/MG*

A-GLYCEROPHOSPHATE DEHYDROGENASE TYPE I FROM RABBIT

A-GLYCEROPHOSPHATE DEHYDROGENASE TYPE*II I FROM RABB

Glycerol-3-PhosphateDehydrogenaseEx

G-3-Pdh,AmmoniumSulfateSuspension,RabbitMuscle

sn-glycero-3-phosphate dehydrogenase from rabbit muscle

sn-glycerol-3-phosphate dehydrogenase from rabbit muscle

α-glycerophosphate dehydrogenase from rabbit muscle

ALPHA-GLYCEROPHOSPHATE DEHYDROGENASE, FROM RABBIT MUSCLE(ALPHA-GDH)

GLYCEROL 3-PHOSPHATE DEHYDROGENASE extrapure

sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase, sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle

α-Glycerophosphate Dehydrogenase from rabbit muscle, GDH, sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase

Glycerol 3-Phosphate Dehydrogenase,Rabbit muscle

Glycerol-3-phosphate dehydrogenase from rabbit, lyophil.

Glycerol-3-Phosphate Dehydrogenase from E. coli, Recombinant

Native Rabbit α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase

Native Rabbit Glycerol-3-phosphate dehydrogenase

Native Rabbit α-Glycerophosphate Dehydrogenase

α-Glycerophosphate Dehydrogenase From Rabbit Muscle

Glycerol-3-Phosphate Dehydrogenase ex. Rabbit Muscle, 15U/mg

9075-65-4

BioChemical

Metabolomics

General Metabolic Enzymes

General Metabolic Enzymes A-H

enzyme