GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE

Product Name: GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
CAS No.: 9001-50-7
Chemical Name: GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
Synonyms: gapd;G3PD;GAPDH;cb609;G-3-PDH;MGC88685;mg:bb02e05;EC: 1.2.1.12;EC 1.2.1.1.2;GAPDH)/CH8530
CBNumber: CB6673129
Molecular Formula: C3H7O6P
Formula Weight: 170.057841
MOL File: 9001-50-7.mol

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GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Property

storage temp.: -20°C
form: suspension
color: white
EPA Substance Registry System: Dehydrogenase, glyceraldehyde phosphate (9001-50-7)

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Safety

Hazard Codes: B,Xi
Risk Statements: 36/37/38
Safety Statements: 26-36
WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: G2267
Product name: Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle
Purity: lyophilized powder, ≥75?units/mg protein
Packaging: 500units
Price: $69.6
Updated: 2024/03/01

Sigma-Aldrich

Product number: SRE0024
Product name: Glyceraldehyde-3-phosphate dehydrogenase human
Purity: recombinant, expressed in Escherichia coli
Packaging: 100μg
Price: $489
Updated: 2024/03/01

Sigma-Aldrich

Product number: G2267
Product name: Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle
Purity: lyophilized powder, ≥75?units/mg protein
Packaging: 1000units
Price: $103
Updated: 2024/03/01

Sigma-Aldrich

Product number: G6019
Product name: Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes
Purity: lyophilized powder, 50-150?units/mg protein
Packaging: 100units
Price: $184.8
Updated: 2024/03/01

Sigma-Aldrich

Product number: G5262
Product name: GAPDH
Purity: standard for protein electrophoresis
Packaging: 1vial
Price: $85.3
Updated: 2024/03/01

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GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Chemical Properties,Usage,Production

Uses

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:

for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
in anti-aggregation assays
in purified GAPDH studies

General Description

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>.

Purification Methods

Purify the dehydrogenase from rabbit muscle by extraction with 0.03N KOH and precipitate it with (NH4)2SO4 (0.52 of saturation). The clear supernatant is adjusted to pH 7.5, and NH3 is added dropwise to pH 8.2-8.4. Crystals appear sometimes even without seeding. The crystals are dissolved in H2O, filtered to remove suspended material and 2 volumes of saturated (NH4)2SO4 at pH 8.2-8.4 is added. After 1hour the crystals appear. Recrystallise it in the same way. [Cori et al. J Biol Chem 173 605 1948, Furfine & Velick J Biol Chem 240 844 1965, The Enzymes 7 243 1963, Lui & Huskey Biochemistry 31 6998 1992.] The Km values are: NADH (3.3WM) and 1,3-diphosphoglycerate (8x10-7M) in pH 7.4 imidazole buffer at 26o, NAD (13WM), glyceraldehyde-3-P (90WM), Pi (2.9x10-4M), and arsenate (69WM) in 8.6 M NaHCO3 buffer at 26oC. [Orsi & Cleland Biochemistry 11 102 1972.]

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Preparation Products And Raw materials

Raw materials

Preparation Products

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GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Suppliers

China 112 Europe 2 Germany 2 Switzerland 1 United States 9 Global 126

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Sigma-Aldrich

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View Lastest Price from GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE manufacturers

Career Henan Chemical Co

Product: GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 9001-50-7
Price: US $1.00/KG
Min. Order: 1KG
Purity: 95%-99%
Supply Ability: 1000kg
Release date: 2018-12-24

9001-50-7, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASERelated Search:

METHYL ISOCYANOACETATE Tosylmethyl isocyanide Ferric acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) TERT-BUTYL ISOCYANIDE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) COBALT(II) ACETYLACETONATE PHENYLSELENOL SALCOMINE Cupric acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) N-BUTYLISOCYANIDE Ethyl isocyanoacetate Benzyl isocyanide 2,4-PENTANEDIONE, SILVER DERIVATIVE Aluminum acetylacetonate

GAPDH

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE

D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE

D-GLYCERALDEHYDE 3-PHOSPHATE: NAD+ OXIDOREDUCTASE [PHOSPHORYLATING]

D-GLYCERALDEHYDE-3-PHOSPHATE: NAD OXIDOREDUCTASE (PHOSPHORYLATING)

EC 1.2.1.1.2

EC: 1.2.1.12

glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle

G-3-PDH

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle lyophilized powder, >=75 units/mg protein

D-Glyceraldehyde 3-phosphate

NAD+ oxidoreductase (phosphorylating)

Dehydrogenase, glyceraldehyde phosphate

GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE FROM RABBI

GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE , FROM BAKE

GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE FROM CHICK

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM HUMAN ERYTHROCYTES

Glyceraldehyde-3-PhosphateDehydrog-

GAPDH)/CH8530

glyceraldehyde-3-phosphate dehydrogenase from baker's yeast (s. cerevisiae)

glyceraldehyde-3-phosphate dehydrogenase from chicken muscle

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, SUSPENSION, FROM RABBIT MUSCLE

GAPDH, D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating)

Glyceraldehyde-3-phosphate Dehydrogenase Agarose from baker's yeast (S.cerevisiae)

glyceraldehyde-3-phoshate dehydrogenase(rabbit muscle)

phosphoglycerine aldehyde dehydrogenase

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

Glyceraldehyde-3-phosphate Dehydrogenase from baker's yeast (S. cerevisiae),D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

Glyceraldehyde-3-phosphate Dehydrogenase from chicken muscle,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

Glyceraldehyde-3-phosphate dehydrogenase human

Triosephosphate dehydrogenase

cb609

gapd

mg:bb02e05

Native Chicken Glyceraldehyde-3-phosphate Dehydrogenase

Native Human Glyceraldehyde-3-phosphate Dehydrogenase

Native Rabbit Glyceraldehyde-3-phosphate Dehydrogenase

glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

Native Bakers yeast (S. cerevisiae) Glyceraldehyde-3-phosphate Dehydrogenase

Human GAPDH Protein, His Tag

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE USP/EP/BP

Glyceraldehyde-3-phosphate dehydrogenase (NAD)

GAPDH from rabbit muscle

G3PD

MGC88685

Glyceraldehyde phosphate dehydrogenase

9001-50-7

General Metabolic Enzymes

General Metabolic Enzymes A-H

Molecular Biology Reagents

Molecular Biology

Metabolomics

BioChemical

Biochemicals