N-Acetylneuraminic Acid Aldolase

Product Name: N-Acetylneuraminic Acid Aldolase
CAS No.: 9027-60-5
Chemical Name: N-Acetylneuraminic Acid Aldolase
Synonyms: C:C&B4;EC 4.1.3.3;Sialate lyase;NANA Aldolase;NEU5AC ALDOLASE;SIALIC ALDOLASE;Sialic acid lyase;Neuraminic aldolase;Neu5Ac Aldolase 001;Neuraminate aldolase
CBNumber: CB6717825
Formula Weight: 0
MOL File: Mol file

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N-Acetylneuraminic Acid Aldolase Property

storage temp.: -20°C
form: powder
color: slightly yellow
Specific Activity: ≥3.0units/mg protein
BRN: 2697172
EPA Substance Registry System: Lyase, acetylneuraminate (9027-60-5)

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 23-24/25-60-37-26-22
WGK Germany: 3
F: 10

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: A6680
Product name: N-Acetylneuraminic Acid Aldolase from microorganisms
Purity: lyophilized powder, ≥20?units/mg protein (biuret)
Packaging: 25units
Price: $606
Updated: 2024/03/01

Usbiological

Product number: 294617
Product name: Sialic acid aldolase
Packaging: 5g
Price: $396
Updated: 2021/12/16

Usbiological

Product number: 294618
Product name: Sialic acid aldolase
Packaging: 10g
Price: $432
Updated: 2021/12/16

American Custom Chemicals Corporation

Product number: ENZ0000886
Product name: N-ACETYLNEURAMINIC ACID ALDOLASE
Purity: 95.00%
Packaging: 5MG
Price: $505.08
Updated: 2021/12/16

Biosynth Carbosynth

Product number: MS11080
Product name: Sialic acid aldolase - crystalline
Packaging: 50g
Price: $525
Updated: 2021/12/16

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N-Acetylneuraminic Acid Aldolase Chemical Properties,Usage,Production

Uses

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Uses

Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes.

General Description

Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens.

Purification Methods

Purify the aldolase by extraction with H2O, protamine precipitation, (NH4)2SO4 fractionation, Me2CO precipitation, acid treatment at pH 5.7 and precipitation at pH 4.5. The equilibrium constant for pyruvate + n-acetyl-D-mannosamine N-acetylneuraminidate at 37o is 0.64. The Km for N-acetylneuraminic acid is 3.9mM in phosphate at pH 7.2 and 37o. [Comb & Roseman Methods Enzymol 5 391 1962.] The enzyme from hog kidney (cortex) has been purified 1700-fold by extraction with H2O, protamine sulfate precipitation, (NH4)2SO4 fractionation, heating between 60-80o, a second (NH4)2SO4 fractionation and starch gel electrophoresis. The Km for N-acetylneuraminic acid is 1.5mM. [Brunetti et al. J Biol Chem 237 2447 1962.]

N-Acetylneuraminic Acid Aldolase Preparation Products And Raw materials

Raw materials

Preparation Products

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N-Acetylneuraminic Acid Aldolase Suppliers

China 60 Germany 2 Japan 3 Switzerland 1 United Kingdom 2 United States 6 Global 74

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Sigma-Aldrich

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9027-60-5, N-Acetylneuraminic Acid AldolaseRelated Search:

Benzyl isocyanide Cupric acetylacetonate 2,4-PENTANEDIONE, SILVER DERIVATIVE METHYL ISOCYANOACETATE SALCOMINE N-BUTYLISOCYANIDE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Ferric acetylacetonate Ethyl isocyanoacetate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Tris(2,4-pentanedionato)chroMiuM(III) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Tosylmethyl isocyanide PHENYLSELENOL Aluminum acetylacetonate TERT-BUTYL ISOCYANIDE COBALT(II) ACETYLACETONATE ALDOLASE

EC 4.1.3.3

N-ACETYLNEURAMINIC ACID ALDOLASE

N-ACETYLNEURAMINIC ACID ALDORASE

N-ACETYLNEURAMINATE PYRUVATE LYASE

NEU5AC ALDOLASE

N-Acetylneuraminic Acid Aldolase from microorganisms

N-ACETYLNEURAMINIC ACID ALDOLASE, FROM MICROORGANISM

N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase

Sialic acid aldolase - Aqueous solution

Sialic acid aldolase - crystalline

Lyase,acetylneuraminate

N-Acetylneuraminic Acid Lyase

N-Acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming)

Sialic Acid Aldolase from Escherichia coli K12

N-Acylneuraminate lyase

N-Acylneuraminic acid aldolase

N-Acylneuraminic aldolase

N-Acylneuraminic lyase

Neuraminate aldolase

Neuraminic acid aldolase

Neuraminic aldolase

Sialate lyase

Sialate pyruvate-lyase

Sialic acid lyase

N-Acylneuraminate aldolase

N-AcetylneuraMinic acid aldolase,froM MicroorganisMi

NANA Aldolase

SIALIC ALDOLASE

SIALIC ACID ALDOLASE

N-Acetylneuraminic acid Aldolase Escherichia coli

N-ACETYLNEURAMINIC ACID ALDOLASE FROMESC HERICHIA C

N-ACETYLNEURAMINIC ACID ALDOLASE >15 U/MG*

n-acetylneuraminic acid aldolase from escherichia coli

Neu5Ac Aldolase 001

Native Microorganism N-Acetylneuraminic acid aldolase

Native Escherichia coli N-Acetylneuraminic Acid Aldolase

Sialic Acid Aldolase from Escherichia coli K12, Recombinant

C:C&B4

N-Acetylneuraminic Acid Aldolase USP/EP/BP

N-Acetylneuraminic Acid Aldolase，NAL

9027-60-5

Post-translational Modification

Proteomics

Proteomics and Protein Expression

Metabolomics

Glycoanalytical Enzymes

General Metabolic Enzymes

Enzymes for Protein Carbohydrate Modification Analysis

Carbohydrate Metabolism

Carbohydrate Analysis

BioChemical

Lyases

Specialty Enzymes