ChemicalBook > CAS DataBase List > Solvent Black 3

Solvent Black 3

Product Name
Solvent Black 3
CAS No.
4197-25-5
Chemical Name
Solvent Black 3
Synonyms
CI 26150;Nsc 11239;Black 2B;sot-black6;BSudan Bla;Sudanblack;CI NO 26150;typogenblack;FAT BLACK HB;Oil Black Hz
CBNumber
CB7198632
Molecular Formula
C29H24N6
Formula Weight
456.54
MOL File
4197-25-5.mol
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Solvent Black 3 Property

Melting point:
120-124 °C(lit.)
Boiling point:
552.68°C (rough estimate)
Density 
1.4899 (rough estimate)
refractive index 
1.4570 (estimate)
storage temp. 
Store at RT.
solubility 
Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol
form 
Powder/Solid
Colour Index 
26150
pka
2.94±0.40(Predicted)
color 
Very dark brown to black
Water Solubility 
Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water.
λmax
598 nm, 415 nm
Merck 
13,8970
BRN 
723248
Stability:
Light Sensitive
Biological Applications
Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening
CAS DataBase Reference
4197-25-5(CAS DataBase Reference)
EPA Substance Registry System
C.I. Solvent Black 3 (4197-25-5)
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Safety

Hazard Codes 
F,Xi
Risk Statements 
11-36/37/38
Safety Statements 
24/25-16-37/39-26
WGK Germany 
3
RTECS 
SD4431500
TSCA 
Yes
HazardClass 
IRRITANT
HS Code 
32041900
Toxicity
LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
199664
Product name
Sudan Black B
Purity
certified by the Biological Stain Commission
Packaging
25g
Price
$96.2
Updated
2024/03/01
TCI Chemical
Product number
S0113
Product name
Sudan Black B
Packaging
25g
Price
$50
Updated
2024/03/01
Alfa Aesar
Product number
J62268
Product name
Sudan Black B
Packaging
10g
Price
$36.5
Updated
2024/03/01
Alfa Aesar
Product number
J62268
Product name
Sudan Black B
Packaging
25g
Price
$69.6
Updated
2024/03/01
Alfa Aesar
Product number
J62268
Product name
Sudan Black B
Packaging
100g
Price
$127
Updated
2024/03/01
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Solvent Black 3 Chemical Properties,Usage,Production

Chemical Properties

It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added.

Uses

Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.

Definition

ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines.

Preparation

aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling.

Safety Profile

A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.

Staining Procedures

1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids.

Properties and Applications

Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.

Standard Light Fastness Heat-resistant(℃) water Sodium Carbonate(5%) Hydrochloric acid(5%)
Melting point Stable
ISO General 126 100 Insoluble No change No change

Solvent Black 3 Preparation Products And Raw materials

Raw materials

Preparation Products

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Solvent Black 3 Suppliers

Apollo Scientific Ltd.
Tel
--
Fax
--
Email
sales@apolloscientific.co.uk
Country
United Kingdom
ProdList
6084
Advantage
88
Avonchem/Chromos Express Ltd.
Tel
--
Fax
--
Email
info@avonchem.co.uk
Country
United Kingdom
ProdList
1189
Advantage
50
Leancare Ltd.
Tel
--
Fax
--
Email
enquiry@leancare.co.uk
Country
United Kingdom
ProdList
6446
Advantage
42
Eurolabs Limited
Tel
--
Fax
--
Country
United Kingdom
ProdList
6309
Advantage
46
MOLEKULA Ltd.
Tel
--
Fax
--
Email
kevinbanks@molekula.com
Country
United Kingdom
ProdList
6140
Advantage
66
VWR International
Tel
--
Fax
--
Email
solutions@vwr.com
Country
United Kingdom
ProdList
6548
Advantage
82
Fisher Scientific UK
Tel
--
Fax
--
Email
sarahr@fisher.co.uk
Country
United Kingdom
ProdList
1157
Advantage
81
Vickers Laboratories Ltd.
Tel
--
Fax
--
Country
United Kingdom
ProdList
776
Advantage
42
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View Lastest Price from Solvent Black 3 manufacturers

Shaanxi Dideu Medichem Co. Ltd
Product
SolventBlack3 4197-25-5
Price
US $1.00-50.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
200000KG
Release date
2024-07-31
Winchem industrial co limited
Product
SOLVENT BLACK 3 4197-25-5
Price
US $0.00/kg
Min. Order
25kg
Purity
0.99
Supply Ability
20MT
Release date
2022-12-20
Zhuozhou Wenxi import and Export Co., Ltd
Product
Solvent Black 3 4197-25-5
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-10

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