Iodonitrotetrazolium chloride

Product Name: Iodonitrotetrazolium chloride
CAS No.: 146-68-9
Chemical Name: Iodonitrotetrazolium chloride
Synonyms: INT;INT DYE;IntA.R.;INT CHLORIDE;2-(p-iodophe;I.N.T. 95% AR;IODONITROTETRAZOLIUM;IODONITROTETRAZOLIUM VIOLET;Iodonitrotetrazolium purple;IODONITRO TETRAZOLIUM (INT)
CBNumber: CB7232772
Molecular Formula: C19H13ClIN5O2
Formula Weight: 505.7
MOL File: 146-68-9.mol

More

Less

Iodonitrotetrazolium chloride Property

Melting point:: 240 °C (dec.)(lit.)
storage temp.: 2-8°C
solubility: methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow
form: Powder, Crystals and/or Chunks
color: White to off-white
Water Solubility: soluble
Sensitive: Light Sensitive
λmax: 248nm
BRN: 4093224
Stability:: Stable. Incompatible with strong oxidizing agents.
Biological Applications: Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer
InChI: InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChIKey: JORABGDXCIBAFL-UHFFFAOYSA-M
SMILES: [N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-]
CAS DataBase Reference: 146-68-9(CAS DataBase Reference)
EPA Substance Registry System: 2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9)

More

Less

Safety

Hazard Codes: Xn,F,Xi
Risk Statements: 20/21/22-68/20/21/22-36/37/38-11
Safety Statements: 36/37-36/37/39-26-22-16
RIDADR: 2811
WGK Germany: 3
F: 3-8-10
TSCA: Yes
HazardClass: 6.1
PackingGroup: III
HS Code: 29339900

More

Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H228Flammable solid

H335May cause respiratory irritation

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P240Ground/bond container and receiving equipment.

P241Use explosion-proof electrical/ventilating/lighting/…/equipment.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

More

Less

N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 58030
Product name: Iodonitrotetrazolium chloride
Purity: BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging: 1g
Price: $218
Updated: 2024/03/01

Sigma-Aldrich

Product number: 58030
Product name: Iodonitrotetrazolium chloride
Purity: BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging: 5G
Price: $763
Updated: 2024/03/01

Sigma-Aldrich

Product number: 58030
Product name: Iodonitrotetrazolium chloride
Purity: BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging: 25g
Price: $2560
Updated: 2024/03/01

TCI Chemical

Product number: I0067
Product name: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride
Purity: >98.0%(HPLC)(T)
Packaging: 1g
Price: $60
Updated: 2024/03/01

TCI Chemical

Product number: I0067
Product name: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride
Purity: >98.0%(HPLC)(T)
Packaging: 5g
Price: $189
Updated: 2024/03/01

More

Less

Iodonitrotetrazolium chloride Chemical Properties,Usage,Production

Description

Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.

Chemical Properties

slightly yellow powder

Uses

Electron acceptor for the colorimetric assay of various dehydrogenases.

Uses

Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator. Hence INT can be used for the colorimetric assay of various dehydrogenases.

Uses

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Definition

ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.

Safety

Iodonitrotetrazolium chloride could cause skin irritation, serious eye irritation, and may cause respiratory irritation.

Properties and Applications

INT (Iodonitrotetrazolium chloride), similar to tetrazolium chloride on reduction, produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine protein concentration in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.

Purification Methods

Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]

Iodonitrotetrazolium chloride Preparation Products And Raw materials

Raw materials

Preparation Products

More

Less

Iodonitrotetrazolium chloride Suppliers

Americas 1 Austria 1 Belgium 1 Canada 3 China 198 Czech Republic 1 Europe 5 France 2 Germany 7 India 18 Japan 5 Poland 1 South Korea 1 Switzerland 5 United Kingdom 17 United States 48 Global 314

Suzhou Rosen Biotechnology Co.,Ltd.

Tel: 0512-87888687 13626191410
Fax: 0512-62929131
Email: rosenchemical@163.com
Country: China
ProdList: 276
Advantage: 58

Jiangsu Hi-Pure Technology New Materials Co. Ltd.

Tel: 0512-62764072; 18051857768
Email: sales@hi-pure.cn
Country: China
ProdList: 35
Advantage: 58

J & K SCIENTIFIC LTD.

Tel: 010-82848833 400-666-7788
Fax: 86-10-82849933
Email: jkinfo@jkchemical.com
Country: China
ProdList: 96815
Advantage: 76

Meryer (Shanghai) Chemical Technology Co., Ltd.

Tel: 021-61259108 18621169109
Fax: 86-21-61259102
Email: market03@meryer.com
Country: China
ProdList: 40228
Advantage: 62

Alfa Aesar

Tel: 400-6106006
Fax: 021-67582001/03/05
Email: saleschina@alfa-asia.com
Country: China
ProdList: 30123
Advantage: 84

TCI (Shanghai) Development Co., Ltd.

Tel: 021-67121386
Fax: 021-67121385
Email: Sales-CN@TCIchemicals.com
Country: China
ProdList: 24529
Advantage: 81

Beijing HwrkChemical Technology Co., Ltd

Tel: 010-89508211 18501085097
Fax: 010-89508210
Email: sales.bj@hwrkchemical.com
Country: China
ProdList: 8415
Advantage: 55

Energy Chemical

Tel: 021-021-58432009 400-005-6266
Fax: 021-58436166
Email: sales8178@energy-chemical.com
Country: China
ProdList: 44689
Advantage: 61

Nanjing Search Biotech Co., Ltd

Tel: +86-025-81682922 +86-13515111588
Fax: 025-85860962
Email: sales@searchbio.com.cn
Country: China
ProdList: 135
Advantage: 68

Nanjing MeiBo Biological Technology Co., Ltd.

Tel: 025-58619198
Fax: 025-58619197
Email: sales@mbbio.com
Country: China
ProdList: 319
Advantage: 60

More

Less

View Lastest Price from Iodonitrotetrazolium chloride manufacturers

Career Henan Chemical Co

Product: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride 146-68-9
Price: US $100.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20kg
Release date: 2018-07-25

Career Henan Chemical Co

Product: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride 146-68-9
Price: US $100.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20kg
Release date: 2018-07-25

146-68-9, Iodonitrotetrazolium chlorideRelated Search:

Methylene Blue trihydrate Thymol Blue RHODAMINE B ISOTHIOCYANATE Tetrabromophenol Blue Evans Blue Solvent Red 43 2,3,5-Triphenyltetrazolium chloride Nitrotetrazolium blue chloride Neotetrazolium chloride Thiazolyl Blue 3-Methyl-2(3H)-benzothiazolone hydrazone hydrochloride hydrate (1:1:?) Acid Blue 90 Tetrazolium Violet Iodobenzene Iodonitrotetrazolium chloride 2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT BCIP/NBT/INT 2-(P-IODOPHENYL)-3-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE HYDRATE,2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE HYDRATE

3-(4-iodophenyl)-2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-iuM chloride

IodonitrotetrazoliuM chloride (INT)

IodonitrotrtrazoliuM chloride

Iodonitrotetrazolium chloride 95%

Iodonitrotetrazolium chloride Vetec(TM) reagent grade, >=98%

Iodonitrotetrazolium chloride Vetec(TM) reagent grade, 95%

INT CHLORIDE

INT DYE

INT

IODONITROTETRAZOLIUM VIOLET

INT~2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride hydrate

Iodonitrotetrazolium chloride ,99%

IODONITROTETRAZOLIUM CHLORIDERESEARCH GRADE

Iodonitrotetrazolium chloride,2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

IntA.R.

2(4-IODOPHENYL)-3(4-NITROPHENYL)-5-PHENYL-2H-Tetrazolium Chloride (INT)extrapure AR

p-Iodonitrotetrazolium Violet, INT, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride

Iodonitrotetrazolium purple

INT, Iodonitrotetrazolium violet

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride hydrate,99%

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride [for Biochemical Research]

2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-3H-tetrazol-2-ium chloride

IODONITROTETRAZOLIUM CHLORIDE (INT DYE)

Iodonitrotetrazolium chloride p.a.

INT, 4-Iodonitrotetrazolium violet

INT Iodonitrotetrazolium Chloride p-Iodonitrotetrazolium Violet

2h-tetrazolium, 2-(piodophenyl)-3-(p-nitrophenyl)-5-phenyl-, Chloride

2-(p-iodophe

2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium:chloride

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazol-3-ium chloride

IODONITRO TETRAZOLIUM (INT)

Iodonitrotetrazolium chloride Ve

P-IODONITROTETRAZOLIUM VIOLET

2H-TETRAZOLIUM, 2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5- PHENYL-, CHLORIDE

2-(P-IODOPHENYL)-3-(P-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE

2-(P-IODOPHENYL)-3-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE

2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE

2-(4'-IODOPHENYL)-3-(4''-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE

2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE

4-IODONITROTETRAZOLIUM VIOLET

3-(P-IODOPHENYL)-2-(P-NITROPHENYL)-5-PHENYL TETRAZOLIUM CHLORIDE

IODONITROTETRAZOLIUM

IODONITROTETRAZOLIUM CHLORIDE

2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2h-tetrazoliuchloride

INT Iodonitrotetrazolium chloride

2-(p-Iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium c

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazoliumChloride[forBiochemicalResearch]&gt

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazoliumChloride&gt

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride(INT) ,≥98%

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium chloride

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chlor...

Iodonitrotetrazolium chloride, for cell culture, 98%+

Iodonitrotetrazolium chloride, for cell culture+

lodonitrotetrazolium chloride

odonitrotetrazolium chloride

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride(INT)

I.N.T. 95% AR

146-68-9