ChemicalBook > CAS DataBase List > Iodonitrotetrazolium chloride

Iodonitrotetrazolium chloride

Product Name
Iodonitrotetrazolium chloride
CAS No.
146-68-9
Chemical Name
Iodonitrotetrazolium chloride
Synonyms
INT;INT DYE;IntA.R.;INT CHLORIDE;2-(p-iodophe;I.N.T. 95% AR;IODONITROTETRAZOLIUM;IODONITROTETRAZOLIUM VIOLET;Iodonitrotetrazolium purple;IODONITRO TETRAZOLIUM (INT)
CBNumber
CB7232772
Molecular Formula
C19H13ClIN5O2
Formula Weight
505.7
MOL File
146-68-9.mol
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Iodonitrotetrazolium chloride Property

Melting point:
240 °C (dec.)(lit.)
storage temp. 
2-8°C
solubility 
methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow
form 
Powder, Crystals and/or Chunks
color 
White to off-white
Water Solubility 
soluble
λmax
248nm
Sensitive 
Light Sensitive
BRN 
4093224
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer
InChI
InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChIKey
JORABGDXCIBAFL-UHFFFAOYSA-M
SMILES
[N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-]
CAS DataBase Reference
146-68-9(CAS DataBase Reference)
EPA Substance Registry System
2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9)
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Safety

Hazard Codes 
Xn,F,Xi
Risk Statements 
20/21/22-68/20/21/22-36/37/38-11
Safety Statements 
36/37-36/37/39-26-22-16
RIDADR 
2811
WGK Germany 
3
F 
3-8-10
TSCA 
Yes
HazardClass 
6.1
PackingGroup 
III
HS Code 
29339900
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H228Flammable solid

H335May cause respiratory irritation

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P240Ground/bond container and receiving equipment.

P241Use explosion-proof electrical/ventilating/lighting/…/equipment.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
58030
Product name
Iodonitrotetrazolium chloride
Purity
BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging
1g
Price
$218
Updated
2024/03/01
Sigma-Aldrich
Product number
58030
Product name
Iodonitrotetrazolium chloride
Purity
BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging
5G
Price
$763
Updated
2024/03/01
Sigma-Aldrich
Product number
58030
Product name
Iodonitrotetrazolium chloride
Purity
BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging
25g
Price
$2560
Updated
2024/03/01
TCI Chemical
Product number
I0067
Product name
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride
Purity
>98.0%(HPLC)(T)
Packaging
1g
Price
$60
Updated
2024/03/01
TCI Chemical
Product number
I0067
Product name
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride
Purity
>98.0%(HPLC)(T)
Packaging
5g
Price
$189
Updated
2024/03/01
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Iodonitrotetrazolium chloride Chemical Properties,Usage,Production

Description

Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.

Chemical Properties

slightly yellow powder

Uses

Electron acceptor for the colorimetric assay of various dehydrogenases.

Uses

Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator. Hence INT can be used for the colorimetric assay of various dehydrogenases.

Uses

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Definition

ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.

Safety

Iodonitrotetrazolium chloride could cause skin irritation, serious eye irritation, and may cause respiratory irritation.

Properties and Applications

INT (Iodonitrotetrazolium chloride), similar to tetrazolium chloride on reduction, produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine protein concentration in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.

Purification Methods

Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]

Iodonitrotetrazolium chloride Preparation Products And Raw materials

Raw materials

Preparation Products

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Iodonitrotetrazolium chloride Suppliers

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View Lastest Price from Iodonitrotetrazolium chloride manufacturers

Career Henan Chemical Co
Product
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride 146-68-9
Price
US $100.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
20kg
Release date
2018-07-25
Career Henan Chemical Co
Product
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride 146-68-9
Price
US $100.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
20kg
Release date
2018-07-25

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  • 146-68-9