Desthiobiotin-PEG4-Alkyne Property

Boiling point:

649.0±50.0 °C(Predicted)

Density 

1.075±0.06 g/cm3(Predicted)

pka

14.93±0.60(Predicted)

Hazard and Precautionary Statements (GHS)

Desthiobiotin-PEG4-Alkyne Chemical Properties,Usage,Production

Description

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.