Basic Orange 14 Property

Melting point:

165 °C

storage temp. 

room temp

solubility 

H2O: soluble1mg/mL, clear, orange to very deep orange

form 

Solid

pka

pKa -3.3(H2O T=room temperature(RT) Iunspeci?ed) (Uncertain);0.2(H2O T=room temperature(RT) Iunspeci?ed) (Uncertain);10.1(H2O T=room temperature(RT) Iunspeci?ed) (Uncertain)

Colour Index 

46005

color 

Red brown

PH

3.9 (10g/l, H2O, 20℃)

Water Solubility 

soluble

Sensitive 

Hygroscopic

BRN 

3734978

Stability:

Stable. Incompatible with strong oxidizing agents. Protect from moisture. Hygroscopic.

CAS DataBase Reference

10127-02-3(CAS DataBase Reference)

Safety

Hazard Codes 

Xn,Xi

Risk Statements 

68-20/21/22-36/37/38

Safety Statements 

45-36/37-36-26

WGK Germany 

3

RTECS 

AR7600000

HS Code 

32041300

Toxicity

A dye used to stain nucleic acids. It fluoresces at 530 nm when intercalated into double stranded DNA, or at 640 nm when ionically bound to single stranded DNA. It produces mutations, some involving reading frame shifts, other deletions or insertions and is carcinogenic. It is also known to be a dermal phototoxicant.

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

Basic Orange 14 Chemical Properties,Usage,Production

Chemical Properties

brown powder

Uses

Fluorescent stain for nucleic acids. An RNA polymerase inhibitorAcridine Orange acts as a lysosomal dye. It is used for cell-cycle studies. It plays an important role as a nucleic acid-selective fluorescent cationic dye, which is useful for cell cycle determination. Further, it serves as an inhibitor of RNA polymerase.

Uses

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

General Description

Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

Biochem/physiol Actions

Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test.