ChemicalBook > CAS DataBase List > EC 3.4.24.4

EC 3.4.24.4

Product Name
EC 3.4.24.4
CAS No.
9036-06-0
Chemical Name
EC 3.4.24.4
Synonyms
PROTEASE;PRONASE;PRONASE E;protease from streptomyces griseus;Proteinase E;Proteline;Pronase-p;Protenaza1;ACTINASE E;PRONASE(R)
CBNumber
CB7670039
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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EC 3.4.24.4 Property

storage temp. 
2-8°C
solubility 
H2O: 5-20 mg/mL
form 
powder
color 
slightly brown
biological source
Streptomyces griseus
Water Solubility 
water: 10mg/mL
Specific Activity
≥70,000proteolytic units/g dry wt
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Safety

Hazard Codes 
Xn
Risk Statements 
36/37/38-42
Safety Statements 
22-24-26-36/37-24/25
WGK Germany 
1
RTECS 
UK9540000
F 
10
HS Code 
2934999090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
P0652
Product name
Protease from Streptomyces sp.
Purity
Type XXI, ≥15?units/mg solid
Packaging
500units
Price
$710
Updated
2025/07/31
Sigma-Aldrich
Product number
7433
Product name
Pronase E
Packaging
1g
Price
$402
Updated
2025/07/31
Sigma-Aldrich
Product number
53702
Product name
Protease from Streptomyces griseus
Packaging
10ku
Price
$76.8
Updated
2025/07/31
Sigma-Aldrich
Product number
53702
Product name
Protease from Streptomyces griseus
Packaging
25ku
Price
$92.2
Updated
2025/07/31
Sigma-Aldrich
Product number
P8811
Product name
Protease from Streptomyces griseus
Purity
powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
Packaging
100mg
Price
$61.6
Updated
2025/07/31
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EC 3.4.24.4 Chemical Properties,Usage,Production

Uses

Pronase E can be used to degrade antheraea pernyi silk fibroin films.

General Description

Note: 1 KU = 1000 units.

Biochem/physiol Actions

This product is a mixture of at least three caseinolytic activities and one aminopeptidase activity. The caseinolytic enzymes were named as Streptomyces griseus Protease A, Streptomyces griseus Protease B and Streptomyces griseus Trypsin. This product may be used when extensive or complete degradation of protein is required. This protease mixture is highly nonspecific and can digest casein to the extent of >70% as mono-amino acids.

in vivo

Experimental Procedure for Isolating Primary Hematopoietic Stem Cells (HSCs) from Mice Using Pronase E[2]1. Animal Anesthesia and Abdominal Exposure: (1) Anesthetize male or female mice. (2) Under sterile conditions, expose the liver via abdominal incision. 2. Liver Perfusion Procedure: (1) Perform liver perfusion through the portal vein using Hanks' Balanced Salt Solution (HBSS) to remove blood and impurities. (2) Conduct enzymatic perfusion with Pronase E and collagenase to dissociate liver tissue. 3. Liver Extraction and Cell Release: (1) Carefully extract the liver from the abdominal cavity using sterile forceps, and quickly transfer it to a sterile culture dish. (2) Gently peel the liver capsule and apply mild mechanical agitation to release cells from the liver tissue. 4. Cell Digestion and Preliminary Centrifugation: (1) Suspend the released cells in a mixed enzyme solution containing Pronase E, collagenase, and DNase, and digest at 37°C for 20 minutes. (2) Perform two rounds of low-speed centrifugation (50 g, 3 minutes) to separate liver cells, and collect the cell suspension. 5. HSC Separation and Density Gradient Centrifugation: (1) Further centrifuge the cell suspension (450 g, 8 minutes) and collect the pellet. (2) Resuspend the pellet in 18% Nycodenz solution as the basis for density gradient separation. (3) Construct a density gradient with 12% Nycodenz, 8.2% Nycodenz, and Gey's Balanced Salt Solution (GBSS). (4) Isolate and purify HSCs from the 8.2% Nycodenz layer through density gradient centrifugation. Note: This protocol provides standard operating procedures; please adjust and optimize according to specific experimental needs and conditions.

EC 3.4.24.4 Preparation Products And Raw materials

Raw materials

Preparation Products

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EC 3.4.24.4 Suppliers

China 201 Europe 2 Germany 3 India 3 Japan 3 Switzerland 1 United Kingdom 4 United States 12 Global 229
Nagase Group
Tel
--
Fax
--
Email
spc@nagase.co.jp
Country
Japan
ProdList
105
Advantage
77
Kanto Chemical Co., Inc.
Tel
--
Fax
--
Email
reag-info@gms.kanto.co.jp
Country
Japan
ProdList
6756
Advantage
74
Nacalai Tesque, Inc.
Tel
--
Fax
--
Email
info-tech@nacalai.co.jp
Country
Japan
ProdList
6046
Advantage
75
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View Lastest Price from EC 3.4.24.4 manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Pronase E 9036-06-0
Price
US $0.00-0.00/g
Min. Order
1g
Purity
98.0%
Supply Ability
1kg/month
Release date
2021-08-26
Career Henan Chemical Co
Product
5-[(3Z)-4,8-Dimethyl-3,7-nonadien-1-yl]octahydro-2H-1,7-dioxacyclopenta[cd]indene-2,6-diyl diacetate 9036-06-0
Price
US $1.00/kg
Min. Order
1kg
Purity
95%-99%
Supply Ability
1000kg
Release date
2018-12-25

9036-06-0, EC 3.4.24.4Related Search:

Tris(2,4-pentanedionato)chroMiuM(III) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE SALCOMINE Ethyl isocyanoacetate Ferric acetylacetonate Benzyl isocyanide TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) COBALT(II) ACETYLACETONATE METHYL ISOCYANOACETATE N-BUTYLISOCYANIDE Cupric acetylacetonate PHENYLSELENOL DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) TERT-BUTYL ISOCYANIDE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Tosylmethyl isocyanide 2,4-PENTANEDIONE, SILVER DERIVATIVE PROTEASE INHIBITOR COCKTAIL

  • s.Griseusproteinase
  • Streptomycesgriseusprotease
  • Streptomycesgriseusproteinase
  • ACTINASE E, STREPTOMYCES GRISEUS
  • Protease from Streptomyces griseus,Actinase E, Pronase E
  • pronasef.streptomycesgriseus
  • Proteinase,streptomycesgriseus
  • Proteline
  • Protenaza1
  • s.Griseusprotease
  • Pronase E froM StreptoMyces griseus ca. 6-8 DMC-U/Mg lyophil.
  • Empynase PD
  • Pronase-p
  • EC 3.4.24.4
  • EC 3.4.24.31
  • EC 3.4.24.32
  • GRISEUS PROTEASE
  • PRONASE F. STREPTOMYCES GRISEUS LYOPH., POWDER, 6 U/MG
  • PROTEASE TYPE XIV BACTERIAL FROM*STREPTO MYCES GRISE
  • PronaseEExStreptomycesGriseus
  • Pronase E, Actinase E
  • Pronase from Streptomyces griseus [EC 3.4.24.4] from Streptomyces griseus
  • Actinase E, Pronase E
  • Type XIV protease
  • ACTINASE E
  • Proteinase E
  • pronase E from streptomyces griseus
  • PRONASE Protease, Streptomycesgriseus, Nuclease-Free
  • protease bacterial (pronase E)*aseptically filled
  • protease from streptomyces griseus
  • protease type xiv bacterial from*streptomyces gri
  • protease type xxi from streptomyces*griseus
  • PROTEASE
  • PROTEASE TYPE IX
  • PROTEASE (STREPTOMYCES GRISEUS)
  • PROTEASE TYPE XIV
  • PROTEASE TYPE XXI
  • PRONASE
  • PRONASE E
  • PRONASE(R)
  • PRONASE(R) PROTEASE
  • 5-[(3Z)-4,8-Dimethyl-3,7-nonadien-1-yl]octahydro-2H-1,7-dioxacyclopenta[cd]indene-2,6-diyl diacetate
  • Protease from Streptomyces sp.
  • PronaseE, 7000U/g
  • Native Streptomyces griseus Protease
  • Pronase E (Activity ≥ 4000 U/mg)
  • Pronase E (Activity ≥ 7000 U/g)
  • Protease ex. Aspergillus Oryzae (Fungal) (Type 1 - Acidic, powder), 500kHUT/g
  • Protease ex. Bacillus Licheniformis (Type 4 - Neutral, powder), 10U/mg
  • Protease ex. Bacillus Sp. (Type 5 - Neutral, granules), 8kNPU/g
  • Protease ex. Bacillus Subtilis (Type 2 - Alkaline, granules), 100kAPU/g
  • Protease ex. Bacillus Subtilis (Type 3 - Alkaline, granules), 1000kAPU/g
  • Protease ex. Bacillus Subtilis (Type 6 - Neutral, powder), 100kNPU/g
  • ~4 units/mg solid， powder
  • Protease from Streptomyces griseus Type XIV
  • Pronase Protease
  • 3.5units/mg
  • Pronase, from Streptomyces griseus