ChemicalBook > CAS DataBase List > GLYCEROKINASE

GLYCEROKINASE

Product Name
GLYCEROKINASE
CAS No.
9030-66-4
Chemical Name
GLYCEROKINASE
Synonyms
GK;GLK;HK4;HHF3;HKIV;EC 2.7.1.30;GLYCEROKINASE;Glyceric kinase;Glycerin kinase;GLYCEROL KINASE
CBNumber
CB8437530
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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GLYCEROKINASE Property

storage temp. 
2-8°C
form 
lyophilized powder
color 
White to off-white
EPA Substance Registry System
Kinase (phosphorylating), glycerol (9030-66-4)
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Safety

Safety Statements 
24/25
WGK Germany 
3
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P285In case of inadequate ventilation wear respiratory protection.

P304+P341IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
WH0002645M1
Product name
Monoclonal Anti-GCK antibody produced in mouse
Purity
clone 3C7, purified immunoglobulin, buffered aqueous solution
Packaging
100μG
Price
$524
Updated
2024/03/01
Sigma-Aldrich
Product number
G6278
Product name
Glycerokinase from Escherichia coli
Purity
300-600?units/mL, ammonium sulfate suspension
Packaging
200units
Price
$237
Updated
2024/03/01
Sigma-Aldrich
Product number
G6142
Product name
Glycerokinase from Cellulomonas sp.
Purity
lyophilized powder, 25-75?units/mg protein
Packaging
1000units
Price
$356
Updated
2024/03/01
Sigma-Aldrich
Product number
G4509
Product name
Glycerokinase from Escherichia coli
Purity
lyophilized powder, 40-100?units/mg protein
Packaging
1000units
Price
$690
Updated
2024/03/01
Sigma-Aldrich
Product number
G0774
Product name
Glycerokinase from Bacillus stearothermophilus
Purity
buffered aqueous solution, ≥75?units/mg protein (biuret)
Packaging
500units
Price
$537
Updated
2024/03/01
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GLYCEROKINASE Chemical Properties,Usage,Production

Chemical Properties

Beige powder

Uses

Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.

Uses

This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.

Uses

Glycerol kinase has been used along with adenosine triphosphate (ATP) for the conversion of glycerol to glycerol-3 phosphate (glycerol-3P) in white adipose tissue (WAT) adipocyte samples by label distribution method. It has also been used along with adenosine triphosphate (ATP) in the derivatization of glycerol to sn-glycerol-3 phosphate (glycerol-3P).

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Purification Methods

Commercial enzyme has been dialysed against 2mM Hepes, 5mM dithiothreitol and 0.3mM EDTA, followed by several changes of 20mM Hepes and 5mM dithiothreitol prior to storage under N2 at -20o. [Knight & Cleland Biochemistry 28 5728 1989.] The enzyme from pigeon liver is purified by acid-precipitation (acetate buffer at pH 5.1), (NH4)2SO4 fractionation, heat treatment (60o/ 1hour), calcium phosphate gel filtration, a second (NH4)2SO4 fractionation, dialysis, elution of inert proteins and crystallisation. This is done by repeatedly extracting the precipitate from the last step with 0.05M sodium pyrophosphate (pH 7.5) containing 1mM EDTA and 0.2M (NH4)2SO4 is added. Careful addition of solid (NH4)2SO4 to this solution leads to crystallisation of the enzyme. Recrystallisation is repeated. The enzyme is activated by Mg2+ and Mn2+ ions and is most stable in solutions in the pH 4.5-5.5 range. The stability is greatly increased in the presence of glycerol. It has Km for glycerol of 60WM and for ATP 9WM in glycine buffer pH 9.8 and 25o. [Kennedy Methods Enzymol 5 476 1962.]

GLYCEROKINASE Preparation Products And Raw materials

Raw materials

Preparation Products

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GLYCEROKINASE Suppliers

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View Lastest Price from GLYCEROKINASE manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Glycerokinase 9030-66-4
Price
US $0.00/KU
Min. Order
10KU
Purity
≥90%
Supply Ability
In stock
Release date
2021-05-28
Hubei Xindesheng Material Technology Co., Ltd.
Product
Glycerin kinase 9030-66-4
Price
US $10.50-7.50/KU
Min. Order
10KU
Purity
≥ 20U/ mg
Supply Ability
10000ku
Release date
2020-11-04
Career Henan Chemical Co
Product
GLYCEROKINASE 9030-66-4
Price
US $1.00/KG
Min. Order
1KG
Purity
95%-99%
Supply Ability
1000kg
Release date
2018-12-24

9030-66-4, GLYCEROKINASERelated Search:

PHENYLSELENOL Ferric acetylacetonate TERT-BUTYL ISOCYANIDE METHYL ISOCYANOACETATE COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Tosylmethyl isocyanide 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE 2,4-PENTANEDIONE, SILVER DERIVATIVE Ethyl isocyanoacetate Tris(2,4-pentanedionato)chroMiuM(III) SALCOMINE Benzyl isocyanide TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Aluminum acetylacetonate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Cupric acetylacetonate GLYCEROKINASE FROM CANDIDA UTILIS

  • Glycerolkinase,E.coli
  • glycerolkinasefromcandidamycoderma
  • GLYCEROKINASE, FROM MICROORGANISM
  • ATP: glycerol 3-phosphotransferase, GK, Glycerokinase from Escherichia coli
  • ATP:glycerol 3-phosphotransferase, Glycerol Kinase
  • Glycerokinase from Streptomyces canus,ATP:glycerol 3-phosphotransferase, Glycerol Kinase
  • Glycerokinase from Escherichia coli,ATP:glycerol 3-phosphotransferase, Glycerol Kinase
  • Glycerokinase from Bacillus stearothermophilus,ATP:glycerol 3-phosphotransferase, Glycerol Kinase
  • Glycerokinase from Cellulomonas sp.,ATP:glycerol 3-phosphotransferase, Glycerol Kinase
  • Glycerokinase [ >=250 units/mg]
  • Glyceric kinase
  • Glycerin kinase
  • Glycerol phosphotransferase
  • GK/Glycerokinase
  • Glycerokinase, 150 units/Mg
  • Anti-MAP4K3, N-Terminal antibody produced in rabbit
  • MAPK/ERK kinase kinase kinase 3
  • MEK kinase kinase 3
  • Anti-GK, C-Terminal antibody produced in rabbit
  • GLK
  • HHF3
  • HK4
  • HKIV
  • Monoclonal Anti-GCK antibody produced in mouse
  • Glycerokinase, 150 units/mg protein
  • Glycerokinase from microorganisms
  • Kinase(phosphorylating),glycerol
  • GK
  • GLYCEROL KINASE
  • GLYCEROKINASE
  • EC 2.7.1.30
  • ATP: GLYCEROL 3-PHOSPHOTRANSFERASE
  • ANTI-GLYCEROL KINASE (GPK2)(C-TERMINAL) antibody produced in rabbit
  • testis specific 2
  • Anti-GK3, N-Terminal antibody produced in rabbit
  • Glycerol Kinase (GK)
  • glycerokinase from bacillus*stearothermophilus so
  • glycerokinase from cellulomonas species
  • glycerokinase from E coli
  • glycerokinase from escherichia coli
  • glycerokinase from streptomyces canus
  • glycerol kinase from E. coli
  • Glycerol Kinase from Escherichia coli
  • GLYCEROL KINASE FROM CANDIDA MYCODERMA, ~85 U/MG*
  • GLYCEROL, PCR REAGENT
  • GLYCEROKINASE FROM BACILLUS*STEAROTHERMO PHILUS SOLU
  • GLYCEROL KINASE FROM E. COLI LYOPH. &
  • GLYCEROKINASE FROM BACILLUS*STEAROTHERMO PHILUS
  • glycerokinase from cellulomonas sp.
  • Native Cellulomonas sp. Glycerokinase
  • Native Escherichia coli Glycerokinase
  • Native Streptomyces canus Glycerokinase
  • Native Microorganism Glycerol Kinase
  • Native Bacillus stearothermophilus Glycerokinase
  • GLYCEROKINASE USP/EP/BP
  • Glycerol Kinase (GK) ex. Microorganism, 30U/mg
  • Glycerol Kinase "Amano" 2
  • 9030-66-4