EC 4.2.2.8

Product Name: EC 4.2.2.8
CAS No.: 149371-12-0
Chemical Name: EC 4.2.2.8
Synonyms: EC 4.2.2.8;EC 4.2.2.7;HEPARITINASE;HEPARINASE II;HEPARITINASE I;HEPARINASE III;HEPARIN LYASE II;Heparin lyasr II;HEPARIN LYASE III;Lyase, heparin, II
CBNumber: CB9267275
Molecular Formula: NULL
Formula Weight: 0
MOL File: Mol file

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EC 4.2.2.8 Property

storage temp.: -20°C
form: lyophilized powder
biological source: bacterial (Flavobacterium heparinum)
Specific Activity: ≥100units/mg protein (enzyme + BSA)

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Safety

Safety Statements: 22-24/25
WGK Germany: 3
F: 10-21

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: H6512
Product name: Heparinase II from Flavobacterium heparinum
Purity: Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100?units/mg protein (enzyme + BSA)
Packaging: 10units
Price: $276
Updated: 2024/03/01

Sigma-Aldrich

Product number: H6512
Product name: Heparinase II from Flavobacterium heparinum
Purity: Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100?units/mg protein (enzyme + BSA)
Packaging: 25units
Price: $598
Updated: 2024/03/01

Sigma-Aldrich

Product number: H6512
Product name: Heparinase II from Flavobacterium heparinum
Purity: Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100?units/mg protein (enzyme + BSA)
Packaging: 100units
Price: $1840
Updated: 2024/03/01

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EC 4.2.2.8 Chemical Properties,Usage,Production

Uses

Heparinase II from Flavobacterium heparinum has been used:

for digestion of heparin sulfate during exosome isolation
for digestion of heparin sulfate in notochordal cell conditioned media (NCCM) to investigate the content of glycosaminoglycans in NCCM
as a component of digestion buffer during cell surface glycan processing
as a component of heparin lyase (HSase) mix to remove the heparin sulfate on the 293ͰT/ACE2 cell surface and study the effect of histones on the infectivity of pseudovirus

Biological Activity

Heparinase II cleaves heparan sulfate, and to a lesser extent heparin (relative activity about 2:1), at the α (1-4) linkages between hexosamines and uronic acid residues (both glucuronic and iduronic), yielding mainly disaccharides. Heparinase II has the broadest substrate specificity of the three heparinases Heparinase enzymes helps in decoding the complex structures of substrates. Heparin interferes with DNA transcription in PCR and in reverse transcription of RNA. Heparinase II has been used to remove heparin for downstream analysis of genomic DNA.

EC 4.2.2.8 Preparation Products And Raw materials

Raw materials

Preparation Products

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EC 4.2.2.8 Suppliers

China 26 Japan 1 Switzerland 1 United States 7 Global 35

Sinopharm Chemical Reagent Co,Ltd.

Tel: 86-21-63210123
Fax: 86-21-63290778 86-21-63218885
Email: sj_scrc@sinopharm.com
Country: China
ProdList: 9815
Advantage: 79

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
ProdList: 51456
Advantage: 80

Raw material medicin reagent co.,Ltd

Tel
Email: sales@njromanme.com
Country: China
ProdList: 4529
Advantage: 58

Career Henan Chemica Co

Tel: +86-0371-86658258 +8613203830695
Fax: 0371-86658258
Email: laboratory@coreychem.com
Country: China
ProdList: 30236
Advantage: 58

BOC Sciences

Tel: +1-631-485-4226
Fax: 1-631-614-7828
Email: inquiry@bocsci.com
Country: United States
ProdList: 19553
Advantage: 58

Wuhan Yanzhe Technology Co., Ltd

Tel: 15527250409
Fax: QQ：2692360204
Email: wenhaotian12@163.com
Country: China
ProdList: 4169
Advantage: 58

Shanghai Yingxin Laboratory Equipment Co., Ltd.

Tel: 021-021-59178156 17002132182
Email: 3146474380@qq.com
Country: China
ProdList: 11603
Advantage: 58

Shanghai Maclean Biochemical Technology Co., LTD

Tel: 021-50706066 15221275939
Email: shenlinxing@macklin.cn
Country: China
ProdList: 30016
Advantage: 58

GlycoSciTech Co. Ltd, Jinan, China

Tel: 18906399270 18906399270
Email: watergreen77@yahoo.com
Country: China
ProdList: 157
Advantage: 58

Shanghai Yingxin laboratory equipment Co., Ltd

Tel: 021-021-59178156 15300768757
Email: 359382369@qq.com
Country: China
ProdList: 10674
Advantage: 58

149371-12-0, EC 4.2.2.8Related Search:

Cupric acetylacetonate COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) N-BUTYLISOCYANIDE METHYL ISOCYANOACETATE PHENYLSELENOL Aluminum acetylacetonate Benzyl isocyanide DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) SALCOMINE Tosylmethyl isocyanide Tris(2,4-pentanedionato)chroMiuM(III) TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) TERT-BUTYL ISOCYANIDE Ferric acetylacetonate 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Ethyl isocyanoacetate EC 4.2.2.8

HEPARIN LYASE II

HEPARIN LYASE III

HEPARITINASE

HEPARITINASE I

HEPARINASE II

HEPARINASE III

HEPARAN SULFATE LYASE I

EC 4.2.2.7

EC 4.2.2.8

heparinase ii from flavobacterium*heparinum

Lyase, heparin, II

Heparinase II from Flavobacterium heparinum,Heparin lyase II

rHeparinase Ⅱ (rHep Ⅱ)

EC 4.2.2.8(HEPARIN LYASE II)

Native Flavobacterium heparinum Heparinase II

Heparinase II from Bacteroides eggerthii, Recombinant

Heparinase II from Flavobacterium heparinum, Recombinant

Heparinase II from Flavobacterium heparinum Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, >=100 units/mg protein (enzyme + BSA)

Heparin lyasr II

149371-12-0

Application Index

Carbohydrate hydrolysis & PTM analysis

Carbohydrate Hydrolysis

Biochemicals and Reagents

BioChemical

Enzymes, Inhibitors, and Substrates