AVIDIN

ChemicalBook > CAS DataBase List > AVIDIN

Product Name: AVIDIN
CAS No.: 1405-69-2
Chemical Name: AVIDIN
Synonyms: AVIDIN;AVIDINE;avidins;Aids104218;Aids-104218;AVIDIN-ACETYL;IMMUNOPURE AVIDIN;AVIDIN, EGG WHITE;Neutralite Avidin;Anti-biotin protein
CBNumber: CB9421365
Molecular Formula: n.a.
Formula Weight: 0
MOL File: Mol file

Less

AVIDIN Property

storage temp.: 2-8°C
form: lyophilized powder
color: White

Less

Safety

Hazard Codes: Xi,Xn
Risk Statements: 20/21/22-36/37/38
Safety Statements: 22-24/25-36-26
WGK Germany: 3
RTECS: CL1590000
F: 10-21
HS Code: 3504009000

Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

Less

N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 189725
Product name: Avidin, Egg White
Packaging: 10mg
Price: $106
Updated: 2024/03/01

Sigma-Aldrich

Product number: 189725
Product name: Avidin, Egg White
Packaging: 100mg
Price: $590
Updated: 2024/03/01

Alfa Aesar

Product number: J64972
Product name: Avidin, from egg
Packaging: 10mg
Price: $144
Updated: 2023/06/20

Sigma-Aldrich

Product number: A9275
Product name: Avidin from egg white
Purity: BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)
Packaging: 1mg
Price: $38.3
Updated: 2024/03/01

Sigma-Aldrich

Product number: A9275
Product name: Avidin from egg white
Purity: BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)
Packaging: 2mg
Price: $57.7
Updated: 2024/03/01

Less

AVIDIN Chemical Properties,Usage,Production

Uses

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Uses

Avidin forms an extremely strong complex with d-biotin (K_d ~ 10^-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.

Definition

A protein occurring in egg white, where it constitutes approximately 0.2% of the total protein. It has the property of combining firmly with biotin and rendering it unavailable to organisms, since proteolytic enzymes do not destroy the avidin–biotin complex. Avidin loses its ability to combine with biotin when subjected to heat; hence cooked egg white does not lead to biotin deficiency.

General Description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Enzyme inhibitor

This bacteriostatic egg-white glycoprotein (MW = 54.7 kDa; CAS 1405- 69-2; Abs10mg/mL = 15.5 at 282nm) contains four identical subunits, each containing a ultrahigh-affinity binding site (Kd ≈ 10?15 M) for biotin, a coenzyme essential in many carboxylation reactions. The avidin-biotin interaction is among the strongest known noncovalent interactions, making it the constant subject of biophysical inquiry. By binding ~15 μg biotin per mg, avidin (which makes up makes up ~0.05% of total egg protein, or around 1.8 mg/egg) deprives invading microorganisms of this vital coenzyme. Many microorganisms partly by-pass this effect by possessing enzymes that covalently link biotin to e-NH2 groups of active-site lysyl residues within biotin-dependent carboxylases. Upon cooking, biotin-free avidin is fully denatured, thus imparting no effect on those consuming cooked eggs. Applications in Cytology: The avidin-biotin interaction provides a simple and sensitive immunoenzymatic method to localize antigens (Ag) in formalin-fixed tissues, typically involving a biotin-labeled secondary antibody (Ab), followed by the addition of avidin-biotin peroxidase complex, and affording superior results over the use of unlabeled antibodies. The availability of biotin-binding sites in the Ab Ag complex is created by the incubation of a relative excess of avidin with biotin-labeled peroxidase. The oligomeric structure of avidin serves to bridge biotin-labeled peroxidase molecules to biotin-labeled peroxidase molecules. When compared on a mol-for-mol basis, the extreme affinity of avidin-biotin interactions rivals or exceeds that of nearly all known Ab-Ag interactions (See also Streptavidin). Biochemical Applications with Recombinant Fusion Proteins: In Escherichia coli, the biotin carboxy carrier protein (BCCP) is biotinylated by BirA, a biotin ligase that covalently attaches a biotin to the amino group of a lysine residue present within the recognition sequence within BCCP. A minimal biotinylation sequence has been found from screens of combinatorial peptide libraries; this 13-residue peptide (Sequence: GLNDIFEAQKIEW), along with a 13- residue long variant (Sequence: GLNDIFEAQKIEWHE), termed the AviTag?, have been identified as effective in vivo and in vitro substrates for the BirA enzyme. When targets proteins are fused to the AviTag and co-expressed in vivo along with BirA, they can be biotinylated in bacteria, yeast, insect, or mammalian cells. Furthermore, when recombinant proteins are fused to the AviTag and incubated in vitro with purified BirA, they can be biotinylated efficiently on the central lysine residue in the AviTag.

Purification Methods

Avidin is purified by chromatography of an ammonium acetate solution on CM-cellulose [Green Biochem J 101 774 1966]. It is also purified by affinity chromatography on 2-iminobiotin-6-aminohexyl-Sepharose 4B [Orr J Biol Chem 256 761 1981]. Itis a biotin-binding protein.

AVIDIN Preparation Products And Raw materials

Raw materials

Preparation Products

Less

AVIDIN Suppliers

China 102 Europe 2 Germany 4 India 3 Japan 2 Switzerland 1 United Kingdom 6 United States 26 Global 146

J & K SCIENTIFIC LTD.

Tel: 010-82848833 400-666-7788
Fax: 86-10-82849933
Email: jkinfo@jkchemical.com
Country: China
ProdList: 96815
Advantage: 76

Adamas Reagent, Ltd.

Tel: 400-6009262 16621234537
Fax: 021-64823266
Email: zhangsn@titansci.com
Country: China
ProdList: 14113
Advantage: 59

BEST-REAGENT

Tel: 400-1166-196 18981987031
Fax: 028-84555506 800101999
Email: cdhxsj@163.com
Country: China
ProdList: 11726
Advantage: 57

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Tel: 0731-85526065 13308475853
Email: ivy@hnhbsj.com
Country: China
ProdList: 4550
Advantage: 62

Beijing HuaMeiHuLiBiological Chemical

Tel: 010-56205725
Fax: 010-65763397
Email: waley188@sohu.com
Country: China
ProdList: 12338
Advantage: 58

Shanghai Aladdin Bio-Chem Technology Co.,LTD

Tel: 400-400-6206333 18521732826
Fax: 021-50323701
Email: market@aladdin-e.com
Country: China
ProdList: 25014
Advantage: 65

Nanjing Sunlida Biological Technology Co., Ltd.

Tel: 025-57798810
Fax: 025-57019371
Email: sales@sunlidabio.com
Country: China
ProdList: 3750
Advantage: 55

Shanghai BeiZhuo Biotech Co., Ltd.

Tel: 021-61119791,13386096464
Fax: 021-50190009
Email: bzswkf@foxmail.com
Country: China
ProdList: 3924
Advantage: 50

Shanghai JONLN Reagent Co., Ltd.

Tel: 400-0066400 13621662912
Fax: 021-55660885
Email: 422131432@qq.com
Country: China
ProdList: 9986
Advantage: 55

Shanghai Macklin Biochemical Co.,Ltd.

Tel: 15221275939 15221275939
Fax: 021-50706099
Email: shenlinxing@macklin.cn
Country: China
ProdList: 15885
Advantage: 55

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
ProdList: 51471
Advantage: 80

Watson Biotechnology Co.,Ltd

Tel: 027-027-59207879 18140587686
Fax: QQ：1972026995
Email: kf@3600chem.com
Country: China
ProdList: 4697
Advantage: 55

Beijing NuoqiYa Biotechnology Co., Ltd.

Tel: 4000-819-385 010-62329685 13718666987
Fax: 010-62340519
Country: China
ProdList: 1971
Advantage: 55

VWR(Shanghai) Co., Ltd

Tel: 400-821-8006
Fax: +86-21-58558801
Email: info_china@vwr.com
Country: China
ProdList: 3041
Advantage: 75

Shanghai Xilong Biochemical Technology Co., Ltd.

Tel: 021-52907766-8042
Fax: 021-52906523
Country: China
ProdList: 9947
Advantage: 58

Shanghai YuanYe Biotechnology Co., Ltd.

Tel: 021-61312847; 18021002903
Fax: QQ:3008007432
Email: 3008007409@qq.com
Country: China
ProdList: 27322
Advantage: 60

Shandong Xiya Chemical Co., Ltd.

Tel: 4009903999 13395398332
Fax: 0539-6365991
Email: sales@xiyashiji.com
Country: China
ProdList: 20810
Advantage: 60

Raw material medicin reagent co.,Ltd

Tel
Email: sales@njromanme.com
Country: China
ProdList: 4534
Advantage: 58

Grader reagent

Tel: 18221735425
Email: sales@xinpingchem.com
Country: China
ProdList: 9951
Advantage: 58

Beijing Solarbio Science & Tecnology Co., Ltd.

Tel: 010-50973130 4009686088
Email: 3193328036@qq.com
Country: China
ProdList: 29796
Advantage: 68

Shanghai Civic Chemical Technology Co., Ltd.

Tel: 021-34053660
Fax: QQ:2881074831
Email: sale2@labgogo.com
Country: China
ProdList: 2018
Advantage: 58

Beijing Jin Ming Biotechnology Co., Ltd.

Tel: 010-60605840 18892239720
Fax: 010-60605840
Email: psaitong@jm-bio.com
Country: China
ProdList: 12308
Advantage: 58

Guangzhou Jhd Chemical Reagent Co., Ltd.

Tel: 020-84383047
Fax: 020-84380294
Email: sales@jhd.com.cn
Country: China
ProdList: 9190
Advantage: 65

Greenherbs Science and Technology Development Co., Ltd.

Tel: 010-61136123
Fax: +86 (10) 8857-9127
Email: pub02@greenherbs.com.cn
Country: China
ProdList: 4049
Advantage: 58

Amatek Scientific Co. Ltd.

Tel: 0512-56316828
Fax: 0512-56316826
Email: info@amateksci.com
Country: China
ProdList: 28820
Advantage: 58

Shandong Ono Chemical Co., Ltd.

Tel: 0539-6362799 20）
Fax: 0539-6362799（To 20）
Country: China
ProdList: 9998
Advantage: 58

Shanghai BaiShun Biological Technology Co., Ltd

Tel: 021-37581181
Fax: 021-57456066
Email: sales@chem-mall.com
Country: China
ProdList: 10335
Advantage: 58

Guangzhou LES biological Technology Co.,Ltd.

Tel: 13672434928
Fax: -
Email: 3307355104@qq.com
Country: China
ProdList: 3650
Advantage: 58

Aikon International Limited

Tel: 025-58859352 18068836627
Fax: 02557626880
Email: sales01@aikonchem.com
Country: China
ProdList: 15085
Advantage: 58

Wuhan FengyaoTonghui Chemical Products Co., Ltd.

Tel: 027-87466105 15377573527
Email: 2678564200@qq.com
Country: China
ProdList: 17997
Advantage: 58

Rhawn Reagent

Tel: 400-400-1332688 18019345275
Fax: 400-133-2688
Email: amy@rhawn.cn
Country: China
ProdList: 15503
Advantage: 58

Tianjin heowns Biochemical Technology Co., Ltd.

Tel: 400-6387771-6039 18920564737
Email: sales@heowns.com
Country: China
ProdList: 20615
Advantage: 60

Shanghai Jizhi Biochemical Technology Co. Ltd.

Tel: 400-400-400-9004166 18616739031
Email: 3007523370@qq.com
Country: China
ProdList: 52712
Advantage: 58

Beijing OKA biological technology co., LTD

Tel: 010-010-62971590 18548936886
Fax: 010-62340519
Email: 3462612863@qq.com
Country: China
ProdList: 6912
Advantage: 58

Shenzhen Polymeri Biochemical Technology Co., Ltd.

Tel: +86-400-002-6226 13028896684
Email: sales@rrkchem.com
Country: China
ProdList: 56039
Advantage: 58

Hefei TNJ Chemical Industry Co.,Ltd.

Tel: 0551-65418671
Fax: 0551-65418697
Email: sales@tnjchem.com
Country: China
ProdList: 34571
Advantage: 58

Zhejiang Lianshuo Biotechnology Co., Ltd.

Tel: 18616526224
Email: lianshuo@vip.126.com
Country: China
ProdList: 9610
Advantage: 58

Tianjin Baima Technology Co., LTD

Tel: 02288195255 15620955089
Email: 362698781@qq.com
Country: China
ProdList: 784
Advantage: 58

Shanghai Hongshun Biological Technology Co., Ltd.

Tel: 15901914505
Fax: 021-54389085
Email: 3187892335@qq.com
Country: China
ProdList: 9102
Advantage: 58

Chongqing Chemdad Co., Ltd

Tel: +86-023-6139-8061 +86-86-13650506873
Email: sales@chemdad.com
Country: China
ProdList: 39916
Advantage: 58

Shanghai jingkang bioengineering co., ltd.

Tel: 021-54721350 13524668266
Fax: 021-5
Email: 2881505714@qq.com
Country: China
ProdList: 7878
Advantage: 58

Shanghai Kanglang Biotechnology Co., Ltd.

Tel: 021-61998208 18217752821
Fax: 021-37680378
Email: sales@klbio.cn
Country: China
ProdList: 9617
Advantage: 58

Absin Bioscience Inc.

Tel: 021-38015121 15000105423
Email: chenjw@absin.cn
Country: China
ProdList: 24734
Advantage: 58

Hunan Wokai Biotechnology Co., Ltd.

Tel: 0744-0744-2221508 13126795102
Fax: 0744-6652010
Email: 2577930172@qq.com
Country: China
ProdList: 319
Advantage: 58

Shanghai Sig Biotechnology Co., LTD

Tel: 021-57810052 18930344717
Fax: 021-57810052
Email: 2089316240@qq.com
Country: China
ProdList: 9396
Advantage: 58

Shanghai Bohu Biotechnology Co., LTD

Tel: 021-57763112 13585886131
Email: 3004987436@qq.com
Country: China
ProdList: 9694
Advantage: 58

Wuhan Yanzhe Technology Co., Ltd

Tel: 15527250409
Fax: QQ：2692360204
Email: wenhaotian12@163.com
Country: China
ProdList: 4576
Advantage: 58

Energy Chemical

Tel: 021-58432009 400-005-6266
Fax: 021-58436166
Email: marketing@energy-chemical.com
Country: China
ProdList: 44941
Advantage: 58

Lingyuan Bebiansen Biological Technology Co., Ltd

Tel: 18642120371
Email: 2837458851@qq.com
Country: China
ProdList: 9798
Advantage: 58

Shandong Tianming Biotechnology Development Co. Ltd

Tel: 400-9019001
Country: China
ProdList: 15070
Advantage: 58

Less

View Lastest Price from AVIDIN manufacturers

Career Henan Chemical Co

Product: AVIDIN 1405-69-2
Price: US $1.00/kg
Min. Order: 1kg
Purity: 95%-99%
Supply Ability: 100kg
Release date: 2018-12-24

1405-69-2, AVIDINRelated Search:

N-BUTYLISOCYANIDE Aluminum acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Ferric acetylacetonate 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE TERT-BUTYL ISOCYANIDE Cupric acetylacetonate Ethyl isocyanoacetate PHENYLSELENOL SALCOMINE Tris(2,4-pentanedionato)chroMiuM(III) COBALT(II) ACETYLACETONATE Tosylmethyl isocyanide TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) METHYL ISOCYANOACETATE Benzyl isocyanide STREPTAVIDIN-HRP

avidins

AVIDIN, HEN EGG WHITE

AVIDINE

AVIDIN, EGG WHITE

AVIDIN CHICKEN EGG WHITE

AVIDIN

AVIDIN-ACETYL

IMMUNOPURE AVIDIN

Anti-biotin protein

AVIDIN, RECOMBINANT, EXPRESSED IN CORN

AVIDIN FROM EGG WHITE

AVIDIN FROM HEN EGG WHITE ~12 U/MG

Avidin from hen egg white

Neutralite Avidin

Aids104218

Aids-104218

Avidin(chiken egg white)

Native Chicken Egg White Avidin

Avidin from egg white recombinant, expressed in corn, >=12 units/mg protein (E1%/280)

Avidin ex. Egg White for molecular biology, 10U/mg Protein

1405-69-2

Proteins and Derivatives

Other

Supplementary Products

Antibodies

Biotin/Avidin

Avidin Reagents

Auxiliary Proteins

Biochemicals and Reagents

BioChemical

proteins

Avidin Reagents

Biotin/Avidin

Supplementary Products

Avidin Biotin MatricesSupplementary Products

Avidin ReagentsPost-Translational Modification

Affinity Chromatography

Affinity ResinsProtein Chromatography

Core Bioreagents

Phosphorylation Analysis

Research Essentials

Resins and Plates

Auxiliary Proteins

Other

Proteins and Derivatives