SPDP Chemical Properties

Melting point:

84-86 °C(lit.)

Boiling point:

465.9±55.0 °C(Predicted)

Density 

1.48±0.1 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

acetone: 50 mg/mL

form 

powder

pka

1.76±0.19(Predicted)

color 

White to Light Yellow

BRN 

1548137

Stability:

Hygroscopic, Moisture Sensitive

InChIKey

JWDFQMWEFLOOED-UHFFFAOYSA-N

CAS DataBase Reference

68181-17-9(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

F 

1-10

HS Code 

29333990

MSDS

• Language:English Provider:SigmaAldrich

SPDP Usage And Synthesis

Description

SPDP NHS ester is a heterobiofunctional crosslinker which can react with amine and sulfhydryl groups. SPDP NHS esters are membrane permeable therefore allowing them to funtion inside of cells.

Chemical Properties

White Solid

Uses

Important crosslinking reagent for the preparation of protein-protein and peptide-protein conjugates linked by disulfide bonds.

Uses

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
It has been used for antibody conjugation and as a protein cross-linker.

Uses

A heterobifunctional cross-linker

General Description

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is a heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. It is typically coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternately, this reagent may be used as a protected thiolating reagent following initial coupling and reduction of linker. It incorporates four atom linker.

SPDP(68181-17-9)Related Product Information

• SPDP-PEG4-acid
• SPDP-PEG6-NHS ester
• SPDP-PEG1-NHS ester
• SPDP-PEG3-COOH
• SPDP-PEG2-NHS ester
• SPDP-PEG6-acid
• SPDP-PEG4-NHS ester
• SPDP-PEG3-NHS ester
• SPDP-PEG8-acid
• SPDP-PEG8-NHS ester
• N-Hydroxysuccinimide
• 2-Pyridinethione
• Pyridinium acetate
• N,N-Diethylhydroxylamine
• N-Acetoxysuccinimide
• Acetohydroxamic acid
• Succinimide
• Argon