HEPES Chemical Properties

Melting point:

234-238 °C

Boiling point:

408℃[at 101 325 Pa]

Density 

1.07 g/mL at 20 °C

vapor pressure 

0Pa at 25℃

refractive index 

n20/D 1.339

storage temp. 

2-8°C

solubility 

H₂O: 1 M at 20 °C, clear, colorless

form 

powder

color 

White

PH Range

7.0 - 7.6

PH

5.0-6.5 (1Maqueous solution)

pka

7.5(at 25℃)

Water Solubility 

Soluble

λmax

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

Merck 

14,4654

BRN 

883043

Stability:

Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.

InChIKey

JKMHFZQWWAIEOD-UHFFFAOYSA-N

LogP

-3.85 at 20℃

CAS DataBase Reference

7365-45-9(CAS DataBase Reference)

EPA Substance Registry System

1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

24/25-22-36-26

WGK Germany 

1

RTECS 

TL6809000

TSCA 

Yes

HS Code 

29335995

MSDS

• Language:English Provider:2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

HEPES Usage And Synthesis

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Uses

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

Uses

HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay. 

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Biological Activity

Multi purpose buffer used in biological research.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

HEPES(7365-45-9)Related Product Information

• 2-Ethylpiperazine
• 1-Ethylpiperazine
• 2-Hydroxyethanesulphonic acid
• 2-(METHYLSULFONYL)ETHANOL
• Ethyl 2-(Chlorosulfonyl)acetate
• N-Aminoethylpiperazine
• 2-Piperidinoethanol
• Hydroxyethyl starch
• Piperazine
• Sodium 2-bromoethanesulphonate
• Hydroxyethyl Cellulose
• Calcium dobesilate
• 1-(2-Hydroxyethyl)piperazine
• 2-(2-Aminoethylamino)ethanol
• Sodium isethionate
• Folic acid
• Piperazine citrate
• Ascoric Acid
• Hepes Buffer
• Hepes Buffered Saline
• Hepes Solution