Desthiobiotin-PEG4-DBCO Chemical Properties

Boiling point:

1013.4±65.0 °C(Predicted)

Density 

1.24±0.1 g/cm3(Predicted)

pka

14.93±0.60(Predicted)

Desthiobiotin-PEG4-DBCO Usage And Synthesis

Description

DBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd?= 1011?vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. DBCO is commonly used for copper-free Click Chemistry reactions.

Desthiobiotin-PEG4-DBCO(2032788-37-5)Related Product Information

• Desthiobiotin-PEG4-NHS ester
• Desthiobiotin-PEG4-Alkyne
• 4-Imidazolidinehexanamide, N-(14-amino-3,6,9,12-tetraoxatetradec-1-yl)-5-methyl-2-oxo-, (4R,5S)-
• DBCO-SE
• DBCO-PEG5-NHS ester
• Diazo Biotin-PEG3-DBCO
• PC DBCO-PEG3-Biotin
• 2-(4-Ethyl-1-piperazinyl)-4-[4-(4-ethyl-1-piperazinyl)phenyl]-5,6,7,8,9,10-hexahydrocycloocta[b]pyridine
• DBCO-PEG4-SE