Desthiobiotin-PEG4-DBCO Chemical Properties

Boiling point:

1013.4±65.0 °C(Predicted)

Density 

1.24±0.1 g/cm3(Predicted)

pka

14.93±0.60(Predicted)

form 

Oil

color 

Light yellow to yellow

Desthiobiotin-PEG4-DBCO Usage And Synthesis

Description

DBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd?= 1011?vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. DBCO is commonly used for copper-free Click Chemistry reactions.

Uses

DBCO-PEG4-Desthiobiotin is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1]. DBCO-PEG4-Desthiobiotin is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC 50

PEGs

References

[1] An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 DOI:10.1016/j.ebiom.2018.09.005

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