Desthiobiotin-PEG4-Alkyne Chemical Properties

Boiling point:

649.0±50.0 °C(Predicted)

Density 

1.075±0.06 g/cm3(Predicted)

pka

14.93±0.60(Predicted)

Desthiobiotin-PEG4-Alkyne Usage And Synthesis

Description

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Uses

Desthiobiotin-PEG4-alkyne is a biotinylated biochemical reagent, which can be utilized in conjunction with click chemistry for the selective labeling and enrichment of certain tRNAs[1].

References

[1] Krasheninina OA, et al., Amine-to-Azide Conversion on Native RNA via Metal-Free Diazotransfer Opens New Avenues for RNA Manipulations. Angew Chem Int Ed Engl. 2021 Mar 22;60(13):6970-6974. DOI:10.1002/anie.202015034

Desthiobiotin-PEG4-Alkyne(1802907-99-8)Related Product Information

• Desthiobiotin-PEG4-DBCO
• BIOTIN HPDP
• DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER
• biotinylamidoethylacetamide
• SULPHOSUCCINIMIDYL-6-(BIOTINAMIDO)HEXANOATE
• (+)-BIOTIN-EPSILON-AMINOCAPROIC ACID
• Succinimidyl 6-(biotinamido)hexanoate
• D-DESTHIOBIOTIN
• BIOTIN-4-FLUORESCEIN
• Methyltetrazine-PEG4-NHS Ester
• Dde Biotin-PEG4-Azide
• Dde Biotin-PEG4-Alkyne
• Methyltetrazine-PEG5-Alkyne
• Bis-sulfone-PEG3-Azide
• Methyltetrazine-PEG4-Azide
• Tetrazine-PEG4-maleimide
• Methyltetrazine-PEG4-Amine
• Methyltetrazine-PEG4-Acid