Sulfo Cy5 Maleimide Chemical Properties

solubility 

Soluble in Water, DMSO, DMF, DCM

Appearance

Dark blue solid

ex/em

649/672 nm (PBS buffer)

ε(extinction coefficient)

271000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.28

InChIKey

ZJKHQSRAFGLFOH-UHFFFAOYSA-N

SMILES

C(N1C(C(C)(C)C2=CC(S(O)(=O)=O)=CC=C12)=CC=CC=CC1=[N+](C2=CC=C(S([O-])(=O)=O)C=C2C1(C)C)C)CCCCC(=O)NCCN1C(C=CC1=O)=O.[KH]

Sulfo Cy5 Maleimide Usage And Synthesis

Description

Cyanine 650 is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. The maleimide can be labeled to thiol containing biomolecules such as primary antibody, secondary antibody, peptide, oligo, etc. for fluorescence imaging and other fluorescence-based biochemical analysis.

Chemical Properties

Appearance: Dark blue solid
ex/em: 649/672 nm (PBS buffer)
Extinction coefficient (ε): 271000 L??mol??1??cm??1
Quantum yield (Φ): 0.28

Uses

Sulfo-Cy5-Mal potassium is a fluorescent dye that can be used to label protein[1].

Chemical Reactivity

Thiol (Cys)

References

[1] V Kholodenko I, et al. Multimerization through Pegylation Improves Pharmacokinetic Properties of scFv Fragments of GD2-Specific Antibodies. Molecules. 2019 Oct 24;24(21):3835. DOI:10.3390/molecules24213835

Abs/Em Maxima

646/662 nm

Fluoresene quantum yield

0.28

Extinction Coefficient

271000

CF260

0.04

CF280

0.04

Spectrally Similar Dyes

Alexa Fluor® 647, DyLight® 649,

Sulfo Cy5 Maleimide(2242791-82-6)Related Product Information

• diSulfo-Cy5 amine
• Cyanine5.5 tetrazine
• Cy5.5 carboxylic acid
• Sulfo-Cyanine5.5 azide
• Sulfo-Cy5.5 amine
• Cyanine5.5 amine
• Cyanine5.5 maleimide
• diSulfo-Cy5 alkyne
• Cyanine5.5 NHS ester
• 3H-IndoliuM, 2-[5-[1-[6-[(3-azidopropyl)aMino]-6-oxohexyl]-1,3-dihydro-3,3-diMethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-triMethyl-5-sulfo-, inner salt
• 3H-Indolium, 2-[5-[1-(5-carboxypentyl)-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl-5-sulfo-, inner salt, potassium salt (1:1)
• Cy5.5 alkyne
• Sulfo-Cy5 NHS Methyl type | Lumiprobe type
• Cy5 DBCO
• Trisulfo-Cy5.5-Alkyne
• Sulfo-Cyanine5 tetrazine
• 1H-Benz[e]indolium, 2-[5-[3-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1,3-trimethyl-, chloride (1:1)