Cyanine5.5 NHS ester Chemical Properties

solubility 

soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)

Appearance

dark blue to violet solid

InChIKey

QJHUOARMFFEDJW-UHFFFAOYSA-N

SMILES

[B+3]([F-])([F-])([F-])[F-].CC1(C(N(CCCCCC(=O)ON2C(CCC2=O)=O)C2C=CC3=CC=CC=C3C1=2)=CC=CC=CC1=[N+](C2C=CC3=CC=CC=C3C=2C1(C)C)C)C

Cyanine5.5 NHS ester Usage And Synthesis

Description

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5® and DyLight 680.

Uses

Cyanine5.5 NHS ester tetrafluoroborate belongs to the cyanine dye series and is a common fluorescent marker for biomolecules that can interact with biomolecules. Cyanine dyes may also bind to double-helical DNA through intercalation and exhibit enhanced fluorescence upon binding.

References

[1] Armitage B A. Cyanine dye–DNA interactions: intercalation, groove binding, and aggregation[J]. DNA Binders and Related Subjects: -/-, 2005: 55-76.

Abs/Em Maxima

684/710 nm

Fluoresene quantum yield

0.2

Extinction Coefficient

198000

CF260

0.07

CF280

0.03

Cyanine5.5 NHS ester(2375105-86-3)Related Product Information

• diSulfo-Cy5 amine
• Cyanine5.5 tetrazine
• Cy5.5 carboxylic acid
• Sulfo-Cyanine5.5 azide
• Sulfo-Cy5.5 amine
• Cyanine5.5 amine
• Cyanine5.5 maleimide
• diSulfo-Cy5 alkyne
• Cyanine5.5 hydrazide
• Cyanine7.5 NHS ester
• Cyanine7 maleimide
• Cyanine5 azide
• Cyanine7 amine
• Cy5 DBCO
• Sulfo Cy5 Maleimide
• Trisulfo-Cy5.5-Alkyne
• Sulfo-Cyanine5 tetrazine
• 1H-Benz[e]indolium, 2-[5-[3-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1,3-trimethyl-, chloride (1:1)