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PJ-34

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PJ-34 Basic information

Product Name:
PJ-34
Synonyms:
  • PJ 34 Hydrochloride
  • N-(5,6-Dihydro-6-oxo-2-phenanthridinyl)-2-acetamidehydrochloride
  • PARP Inhibitor VIII, PJ34
  • PJ-34 hydrate hydrochloride
  • CS-723
  • PJ 34;PJ-34
  • PI34
  • 2-(dimethylamino)-N-(6-oxo-5H-phenanthridin-2-yl)acetamide,hydrochloride
CAS:
344458-15-7
MF:
C17H17N3O2HCl
MW:
0
Product Categories:
  • Amines
  • Aromatics
  • Heterocycles
  • Inhibitors
  • Intermediates & Fine Chemicals
  • Pharmaceuticals
Mol File:
344458-15-7.mol
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PJ-34 Chemical Properties

storage temp. 
Keep in dark place,Inert atmosphere,Store in freezer, under -20°C
solubility 
H2O: soluble22mg/mL
form 
White solid
color 
white to yellow
Sensitive 
Light Sensitive & Hygroscopic
Stability:
Stable for 2 years from date of purchase as supplied. Solutions in DMSO or distilled water may be stored at -20° for up to 3 months.
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Safety Information

Safety Statements 
22-24/25
WGK Germany 
3
HS Code 
29339900
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PJ-34 Usage And Synthesis

Description

PJ-34 HCl (344458-15-7) is a potent and selective inhibitor of PARP1 and 2. EC50 = 20 nM.1?Reduces ischemia reperfusion injury in a mouse model.2 Displays anti-inflammatory effects in a transient focal cerebral ischemia mouse model.3 PJ-34 HCl causes PARP1 independent, p21 dependent mitotic arrest.4

Uses

A poly adenosine diphosphate-ribose polymerase inhibitor, attenuates chromate-induced nephrotoxicity. PJ-34 has a lethal effect on breast cancer cells, both in vitro and in vivo, in particular on breast cancer MCF-7.

Uses

PJ-34 hydrochloride hydrate has been used:

  • as a poly(ADP-ribose) polymerase (PARP) inhibitor 1 in rats to test the effect of PAPR1 in neuropathic pain
  • as a component of protein extraction buffer to enable visualization of the high-molecular-weight smear of PARylated proteins in various cell samples
  • as PARP inhibitor in murine MLE-12 epithelial cell line

Definition

ChEBI: PJ34 hydrochloride is a hydrochloride salt prepared from equimolar amounts of PJ34 and hydrochloric acid. It has a role as an angiogenesis inhibitor, an anti-inflammatory agent, an antiatherosclerotic agent, an antineoplastic agent, an apoptosis inducer, a cardioprotective agent, an EC 2.4.2.30 (NAD(+) ADP-ribosyltransferase) inhibitor and a neuroprotective agent. It contains a PJ34(1+).

General Description

A cell-permeable, water-soluble phenanthridinone-derivative that acts as a potent inhibitor of poly(ADP-ribose) polymerase (PARP; EC50 = 20 nM). Shown to be about 10,000 times more potent than the prototypical PARP inhibitor, 3-Aminobenzamide (Cat. No. 165350; EC50 = 200 μM). Does not act as an antioxidant at higher concentrations (1 μM to 10 mM). Exhibits neuroprotection in in vivo and in vitro models of stroke. Also available as a 20 mM solution in H2O(Cat. No. 528151).

Biological Activity

Potent inhibitor of poly(ADP-ribose) polymerase (PARP) (EC 50 = 20 nM). ~ 1000-fold more potent than 3-Aminobenzamide . Protects primary neuronal cells from oxygen-glucose deprivation in vitro and reduces infarct size following focal cerebral ischemia in vivo .

Biochem/physiol Actions

Cell permeable: yes

storage

+4°C

References

1) Pellicciari et al. (2008), On the way to selective PARP-2 inhibitors. Design, synthesis, and preliminary evaluation of a series of isoquinolinone derivatives; Chem. Med. Chem., 3 914 2) Crawford et al. (2010), Postischemic poly (ADP-ribose) polymerase (PARP) inhibition reduces ischemia reperfusion injury in a hind-limb ischemia model; Surgery, 148 110 3) Haddad et al. (2006), Anti-inflammatory effects of PJ34, a poly(ADP-ribose) polymerase inhibitor, in transient focal cerebral ischemia in mice; Br. J. Pharmacol., 149 23 4) Madison et al. (2011), The PARP inhibitor PJ34 causes a PARP1-independent, p21 dependent mitotic arrest; DNA Repair, 10 1003

PJ-34Supplier

Shanghai Boyle Chemical Co., Ltd.
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J & K SCIENTIFIC LTD.
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010-82848833 400-666-7788
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3B Pharmachem (Wuhan) International Co.,Ltd.
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821-50328103-801 18930552037
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VDM Biochemicals
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0330-2528181
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Chemsky (shanghai) International Co.,Ltd
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021-50135380
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