4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide
- Product Name
- 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide
- CAS No.
- 312756-74-4
- Chemical Name
- 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide
- Synonyms
- RS-1;CS-2312;RS1;RS 1;RAD51-stimulatory compound 1;RAD51-Stimulatory Compound-1, RS-1;RS-1 - RAD51-Stimulatory Compound-1;RAD51,RS1,RS-1,inhibit,Inhibitor,RS 1,CRISPR/Cas9;3-benzylsulfamoyl-4-bromo-N-(4-bromophenyl)benzamide;3-(N-Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide;RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem
- CBNumber
- CB02695334
- Molecular Formula
- C20H16Br2N2O3S
- Formula Weight
- 524.23
- MOL File
- 312756-74-4.mol
4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Property
- Density
- 1.680±0.06 g/cm3(Predicted)
- storage temp.
- room temp
- solubility
- DMSO: ≥10mg/mL
- form
- powder
- pka
- 10.16±0.30(Predicted)
- color
- off-white to light tan
- Stability:
- Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months.
- InChIKey
- VBOWCZDVEOFGRY-UHFFFAOYSA-N
- CAS DataBase Reference
- 312756-74-4
N-Bromosuccinimide Price
- Product number
- R9782
- Product name
- RS-1
- Purity
- ≥98% (HPLC)
- Packaging
- 5mg
- Price
- $44.4
- Updated
- 2024/03/01
- Product number
- 553510
- Product name
- RAD51-Stimulatory Compound-1, RS-1
- Packaging
- 10mg
- Price
- $155
- Updated
- 2024/03/01
- Product number
- 21037
- Product name
- RS-1
- Purity
- ≥95%
- Packaging
- 5mg
- Price
- $32
- Updated
- 2024/03/01
- Product number
- 21037
- Product name
- RS-1
- Purity
- ≥95%
- Packaging
- 10mg
- Price
- $57
- Updated
- 2024/03/01
- Product number
- R9782
- Product name
- RS-1
- Purity
- ≥98% (HPLC)
- Packaging
- 25mg
- Price
- $449
- Updated
- 2024/03/01
4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Chemical Properties,Usage,Production
Description
RS-1 is an activator of DNA repair protein RAD51 (Kd = 48-107 for human RAD51 with different cofactors present). It stimulates homologous strand assimilation activity at least 5- to 11-old, enhancing homologous recombination activity of hRAD51. RS-1 is a potent enhancer of CRISPR-mediated genome editing, increasing homology directed repair 3- to 6-fold. It is used to increase CRISPR-mediated knock-in efficiencies in vitro and in vivo.
Uses
4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]benzamide is a stimulant of the human homologous recombination protein RAD51.
Uses
RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.
General Description
A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis. Although RS-1 enhances hRAD51 filament formation on ssDNA with or without the cofactor NTP, active filaments and recombinase activity are only induced in the presence of ATP or AMP-PNP, but not with ADP or no cofactors. Shown to promote resistance of primary human neonatal dermal fibroblasts to Cisplatin- (Cat. No. 232120) induced death in a dose-dependent manner. RS-1 is inactive toward related DNA strand exchange proteins scRAD5 and scDMC1 of yeast origin or E. coli RedA.This HDR (homology-directed repair) enhancer, is shown to significantly increase both Cas9 & TALEN-mediated knock-in efficiencies.
Biological Activity
RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer that enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.
Biochem/physiol Actions
RS-1 is a sulfonamido-benzamide compound.
in vitro
RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity[1]. RS-1 enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.
storage
Room temperature
References
1) Jayathilaka et al. (2008), A chemical compound that stimulates the human homologous recombination protein RAD51; Proc. Natl. Acad. Sci. USA 105 15848 2) Mason et al. (2014), The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors; Cancer Res. 74 3546 3) Pinder et al. (2015), Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing; Nucleic Acids Res. 43 9379 4) Song et al. (2016), RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency; Nat. Commun. 7 10548
4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Preparation Products And Raw materials
Raw materials
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View Lastest Price from 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide manufacturers
- Product
- 3-(Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide 312756-74-4
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- Min98% HPLC
- Supply Ability
- g/kg/ton
- Release date
- 2020-01-01