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CARBOXYPEPTIDASE A

Product Name
CARBOXYPEPTIDASE A
CAS No.
11075-17-5
Chemical Name
CARBOXYPEPTIDASE A
Synonyms
EC 3.4.17.1;E.C. 3.4.2.1;E.C. 3.4.12.2;CARBOXYPEPTIDASE A;CARBOXYPOLYPEPTIDASE;Carboxypeptidase A?Agarose;PEPTIDYL-L-AMINO-ACID HYDROLASE;CARBOXYPEPTIDASE A TYPE II-PMSF;CARBOXYPEPTIDASE A, PMSF TREATED;CarboxypeptidaseAExBovinePanceas
CBNumber
CB1776427
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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CARBOXYPEPTIDASE A Property

Density 
1.00 g/mL at 20 °C
Flash point:
4 °C
storage temp. 
2-8°C
form 
suspension
color 
white
CAS DataBase Reference
11075-17-5
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Safety

Hazard Codes 
F,Xn,B,Xi
Risk Statements 
11-38-42-48/20-63-65-67-36-36/37/38
Safety Statements 
36/37-45-62-36-26-23-16-24-22
RIDADR 
UN 1294 3/PG 2
WGK Germany 
2
F 
10
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
SAE0046
Product name
Carboxypeptidase A from bovine pancreas
Purity
(Type II-PMSF treated), ≥20?units/mg protein
Packaging
500units
Price
$46.8
Updated
2024/03/01
Sigma-Aldrich
Product number
SAE0046
Product name
Carboxypeptidase A from bovine pancreas
Purity
(Type II-PMSF treated), ≥20?units/mg protein
Packaging
2500Units
Price
$133
Updated
2024/03/01
Sigma-Aldrich
Product number
SAE0046
Product name
Carboxypeptidase A from bovine pancreas
Purity
(Type II-PMSF treated), ≥20?units/mg protein
Packaging
5000units
Price
$235
Updated
2024/03/01
Sigma-Aldrich
Product number
C9268
Product name
Carboxypeptidase A from bovine pancreas
Purity
(Type II-PMSF treated), ≥50?units/mg protein, aqueous suspension
Packaging
500units
Price
$143
Updated
2024/03/01
Sigma-Aldrich
Product number
C9268
Product name
Carboxypeptidase A from bovine pancreas
Purity
(Type II-PMSF treated), ≥50?units/mg protein, aqueous suspension
Packaging
2500Units
Price
$270
Updated
2024/03/01
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CARBOXYPEPTIDASE A Chemical Properties,Usage,Production

Chemical Properties

Suspension (white)

Uses

The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.

Definition

An enzyme that catalyzes the cleavage of amino acids from a protein at the C-terminus of a polypeptide chain.

General Description

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Industrial uses

Carboxypeptidase A(CPA) is an enzyme of the digestive system that is known to cleave amino acids favouring the C-terminal end as well as certain esters. This enzymatic activity depends on the metal at the catalytic site. Zn2+ and some Co2+-containing CPAs exhibit peptidase function, whilst esterase function has been seen by CPAs containing a variety of divalent d-block metals. CPA has a size similar to CA, consisting of about 300 amino acids and a molecular mass of 34 kDa.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.

Purification Methods

Carboxypeptidase A is purified by DEAE-cellulose chromatography, activation with trypsin and dialysed against 0.1M NaCl, yielding crystals. It is recrystallised by dissolving in 20 mL of M NaCl and dialysed for 24hours each against the following salts present in 500mL of 0.02M sodium veronal pH 8.0, 0.5M NaCl, 0.2M NaCl and 0.15M NaCl. The last dialysate usually induces crystallisation. If it does not crystallise, then dialyse the last solution against 0.02M sodium veronal containing 0.10M NaCl. Only 2 or 3 recrystallisations are required to attain maximum activity. [Cox et al. Biochemistry 3 44 1964.] Enzyme activity is measured by hydrolysing hippuryl-L-phenylalanine (or phenylacetic acid) and observing the rate of change of optical density at 254nm (reaction extinction coefficient is ~0.592 cm2/Wmole at pH 7.5) [Bergmyer Methods in Enzymatic Analysis (Academic Press) 1 436 1974].

CARBOXYPEPTIDASE A Preparation Products And Raw materials

Raw materials

Preparation Products

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CARBOXYPEPTIDASE A Suppliers

China 54 Europe 2 Germany 2 Switzerland 1 United Kingdom 1 United States 7 Global 67
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
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3bsc@sina.com
Country
China
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15848
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Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
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021-64823266
Email
zhangsn@titansci.com
Country
China
ProdList
14113
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Guangzhou Isun Pharmaceutical Co., Ltd
Tel
020-39119399 18927568969
Fax
020-39119999
Email
isunpharm@qq.com
Country
China
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4428
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Nanjing Sunlida Biological Technology Co., Ltd.
Tel
025-57798810
Fax
025-57019371
Email
sales@sunlidabio.com
Country
China
ProdList
3750
Advantage
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Shanghai BeiZhuo Biotech Co., Ltd.
Tel
021-61119791,13386096464
Fax
021-50190009
Email
bzswkf@foxmail.com
Country
China
ProdList
3924
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50
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
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China
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Beijing NuoqiYa Biotechnology Co., Ltd.
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4000-819-385 010-62329685 13718666987
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China
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Shanghai YuanYe Biotechnology Co., Ltd.
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021-61312847; 18021002903
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QQ:3008007432
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China
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Raw material medicin reagent co.,Ltd
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025-57798860
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sales@njromanme.com
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Guangzhou LES biological Technology Co.,Ltd.
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11075-17-5, CARBOXYPEPTIDASE ARelated Search:

TERT-BUTYL ISOCYANIDE Cupric acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE SALCOMINE PHENYLSELENOL N-BUTYLISOCYANIDE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Tosylmethyl isocyanide Ferric acetylacetonate METHYL ISOCYANOACETATE Aluminum acetylacetonate COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Ethyl isocyanoacetate Tris(2,4-pentanedionato)chroMiuM(III) Benzyl isocyanide CARBOXYPEPTIDASE B

  • Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase from bovine pancreas
  • Carboxypeptidase A from bovine pancreas,Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
  • Peptidyl-L-amino-acid hydrolase from bovine pancreas
  • Carboxypeptidase A?Agarose
  • E.C. 3.4.12.2
  • E.C. 3.4.2.1
  • PEPTIDYL-L-AMINO-ACID HYDROLASE
  • PEPTIDYL-L-AMINO-ACID HYDROLASE TYPE II-PMSF
  • EC 3.4.17.1
  • CARBOXYPOLYPEPTIDASE
  • CARBOXYPOLYPEPTIDASE TYPE II-PMSF
  • CARBOXYPEPTIDASE A
  • CARBOXYPEPTIDASE A, BOVINE PANCREAS
  • CARBOXYPEPTIDASE A, PMSF TREATED
  • CARBOXYPEPTIDASE A TYPE II-PMSF
  • carboxypeptidase A from bovine pancreas stab.
  • carboxypeptidase A type I from*bovine pancreas
  • carboxypeptidase A-pmsf from*bovine pancreas type
  • CARBOXYPEPTIDASE A FROM BOVINE PANCREAS STAB., ~70 U/MG*
  • CARBOXYPEPTIDASE A FROM HUMAN PANCREAS
  • CARBOXYPEPTIDASE A FROM BOVINE PANCREAS, ~1100 U/ML
  • CARBOXYPEPTIDASE A TYPE II2X CRYSTALLIZE D
  • CARBOXYPEPTIDASE A-PMSF FROMBOVINE PANCR EAS TYPE I
  • CarboxypeptidaseAExBovinePanceas
  • carboxypeptidaseaphenylmethylsulfonylfluoridetreated
  • carboxypeptidase a from bovine pancreas
  • Carboxypeptidase A, PMSF treated from bovine pancreas from bovine pancreas
  • Carboxypeptidase A from bovine pancreas [EC 3.4.17.1] from bovine pancreas
  • Peptidyl-L-amino-acid hydrolase from human pancreas
  • Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
  • Native Bovine Carboxypeptidase A
  • Carboxypeptidase A from bovine pancreas (Type II-PMSF treated), >=50 units/mg protein, aqueous suspension
  • 11075-17-5
  • BioChemical
  • Enzymes, Inhibitors, and Substrates
  • Proteolytic Enzymes and Substrates
  • Proteolytic Enzymes
  • Biochemicals and Reagents