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DAF-FM DA

Product Name
DAF-FM DA
CAS No.
254109-22-3
Chemical Name
DAF-FM DA
Synonyms
DAF-FM DA;DAF-FM DA solution;-[9H]xanthen]-3-one;DAF-FM DA (cell permeable);DAF-FM DA [DAF-FM Diacetate];-difluoro-spiro[isobenzofuran-1(3H);Diaminofluorescein-FM diacetate;DAF-FM Diacetate NO fluorescent probe;DAF-FM DA - CAS 254109-22-3 - Calbiochem;4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy)
CBNumber
CB21074275
Molecular Formula
C25H18F2N2O7
Formula Weight
496.42
MOL File
254109-22-3.mol
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DAF-FM DA Property

Boiling point:
695.7±55.0 °C(Predicted)
Density 
1.56±0.1 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
Soluble in dimethyl sulfoxide
pka
4.20 ± 0.20, most basic, temperature: 25 °C
form 
Pale yellow solution
color 
clear clear, colorless
λmax
365 nm (MeOH)
Biological Applications
Detecting bacteria/microorganisms; nitric oxide indicator,
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
D1946
Product name
DAF-FM DA
Purity
≥98% (HPLC)
Packaging
1mg
Price
$737
Updated
2024/03/01
Sigma-Aldrich
Product number
251520-M
Product name
DAF-FM DA - CAS 254109-22-3 - Calbiochem
Purity
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
Packaging
251520-1MG
Price
$1010
Updated
2024/03/01
Sigma-Aldrich
Product number
251520-M
Product name
DAF-FM DA - CAS 254109-22-3 - Calbiochem
Purity
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
Packaging
1mg
Price
$957
Updated
2023/01/07
Cayman Chemical
Product number
18767
Product name
DAF-FM diacetate
Purity
≥98%
Packaging
1mg
Price
$413
Updated
2024/03/01
Cayman Chemical
Product number
18767
Product name
DAF-FM diacetate
Packaging
5mg
Price
$1543
Updated
2024/03/01
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DAF-FM DA Chemical Properties,Usage,Production

Uses

DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495

Uses

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Biochem/physiol Actions

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

storage

Store at -20°C

DAF-FM DA Preparation Products And Raw materials

Raw materials

Preparation Products

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DAF-FM DA Suppliers

Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
Country
China
ProdList
4654
Advantage
58
Artis Chemistry (Shanghai) Co. Ltd.
Tel
86-21-60936353
Fax
86-21-60936352
Country
China
ProdList
336
Advantage
58
Haoyuan Chemexpress Co., Ltd.
Tel
021-58950125
Fax
(86) 21-58955996
Email
info@chemexpress.com
Country
China
ProdList
7553
Advantage
61
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
18521735133 18521732826;
Fax
021-50323701
Email
market@aladdin-e.com
Country
China
ProdList
25015
Advantage
65
MedChemexpress LLC
Tel
021-58955995
Fax
609-228-5909
Email
sales@medchemexpress.cn
Country
United States
ProdList
4863
Advantage
58
LETOPHARM LIMITED
Tel
+86-21-5821 5861
Fax
+86-21-5106 2861
Email
sales@letopharm.com
Country
China
ProdList
2384
Advantage
58
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51471
Advantage
80
Shanghai EFE Biological Technology Co., Ltd.
Tel
021-65675885 18964387627
Fax
021-65675885
Email
info@efebio.com
Country
China
ProdList
9709
Advantage
58
Chengdu Dianchun Technology Co., Ltd
Tel
400-1166-196 18502815961
Fax
QQ:800101999
Email
cdhxsj@163.com
Country
China
ProdList
14623
Advantage
60
Beijing Solarbio Science & Tecnology Co., Ltd.
Tel
010-50973130 4009686088
Email
3193328036@qq.com
Country
China
ProdList
29797
Advantage
68

254109-22-3, DAF-FM DARelated Search:


  • 4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy)
  • 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
  • DAF-FM DA solution
  • DAF-FM DA (cell permeable)
  • -[9H]xanthen]-3-one
  • Diaminofluorescein-<WBR>FM diacetate
  • -difluoro-spiro[isobenzofuran-1(3H)
  • DAF-FM DA
  • DAF-FM DA - CAS 254109-22-3 - Calbiochem
  • 4-Amino-2',7'-Difluoro-5-(Methylamino)-3-Oxo-3H-Spiro[2-Benzofuran-1,9'-Xanthene]-3',6'-Diyl Diacetate
  • DAF-FM DA [DAF-FM Diacetate]
  • 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein diacetate
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-4-amino-2',7'-difluoro-5-(methylamino)-
  • DAF-FM Diacetate NO fluorescent probe
  • 6-acetyloxy-4-amino-2,7-difluoro-5-(methylamino)-3-oxospiro2-benzofuran-1,9-xanthene-3-yl acetate
  • 254109-22-3