PNGASE F

Product Name: PNGASE F
CAS No.: 83534-39-8
Chemical Name: PNGASE F
Synonyms: PNGASE A;PNGASE F;EC 3.5.1.52;N-GLYCANASE;GLYCOPEPTIDASE;N-GLYCANASE(R);PNGase F（Fast）;N-GLYCOSIDASE A;N-GLYCOSIDASE F;GLYCOPEPTIDASE A
CBNumber: CB2114680
Formula Weight: 0
MOL File: Mol file

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PNGASE F Property

storage temp.: 2-8°C
form: buffered aqueous solution
color: Colorless to light yellow

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Safety

Hazard Codes: Xi,N,Xn
Risk Statements: 37/38-41-50/53-43-20/21/22
Safety Statements: 22-24/25-36/37/39-26-61
RIDADR: UN 3316 9
WGK Germany: 2

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: P7367
Product name: PNGase F from Elizabethkingia meningoseptica
Purity: BioReagent, ≥95% (SDS-PAGE), for proteomics
Packaging: 50units
Price: $407
Updated: 2024/03/01

Sigma-Aldrich

Product number: P7367
Product name: PNGase F from Elizabethkingia meningoseptica
Purity: BioReagent, ≥95% (SDS-PAGE), for proteomics
Packaging: 300Units
Price: $1800
Updated: 2024/03/01

Sigma-Aldrich

Product number: G5166
Product name: PNGase F from Elizabethkingia miricola
Purity: buffered aqueous solution
Packaging: 50units
Price: $330
Updated: 2024/03/01

Sigma-Aldrich

Product number: G5166
Product name: PNGase F from Elizabethkingia miricola
Purity: buffered aqueous solution
Packaging: 100units
Price: $540
Updated: 2024/03/01

Sigma-Aldrich

Product number: G1549
Product name: PNGase F from Elizabethkingia meningoseptica
Purity: buffered aqueous solution, recombinant, expressed in E. coli
Packaging: 50units
Price: $272
Updated: 2024/03/01

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PNGASE F Chemical Properties,Usage,Production

Uses

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Uses

PNGase F from Elizabethkingia meningoseptica has been used:

for de-N-glycosylation of Zika20virus E protein
to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Uses

Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.

General Description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGASE F Preparation Products And Raw materials

Raw materials

Preparation Products

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PNGASE F Suppliers

China 48 Japan 3 Switzerland 1 Ukraine 1 United Kingdom 1 United States 8 Global 62

TargetMol Chemicals Inc.

Tel
Email: support@targetmol.com
Country: United States
ProdList: 38470
Advantage: 58

Aladdin Scientific

Tel: +1-+1(833)-552-7181
Email: sales@aladdinsci.com
Country: United States
ProdList: 52925
Advantage: 58

Creative Enzymes

Tel: 1-516-855-7709
Fax: 1-631-938-8127
Email: info@creative-enzymes.com
Country: United States
ProdList: 8748
Advantage: 58

Creative Enzymes

Tel: --
Fax: --
Email: info@creative-enzymes.com
Country: United States
ProdList: 6057
Advantage: 58

Riedel-de Haen AG

Tel: --
Fax: --
Country: United States
ProdList: 6773
Advantage: 87

EMD Biosciences, Inc.

Tel: --
Fax: --
Email: technical@calbiochem.com
Country: United States
ProdList: 6529
Advantage: 66

MP Biomedicals, Inc.

Tel: --
Fax: --
Country: United States
ProdList: 6561
Advantage: 81

Roche Applied Science

Tel: --
Fax: --
Email: biochemts.us@roche.com
Country: United States
ProdList: 696
Advantage: 85

83534-39-8, PNGASE FRelated Search:

METHYL ISOCYANOACETATE Ethyl isocyanoacetate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Benzyl isocyanide TERT-BUTYL ISOCYANIDE Cupric acetylacetonate 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Aluminum acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III) Ferric acetylacetonate PHENYLSELENOL TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Tosylmethyl isocyanide SALCOMINE N-BUTYLISOCYANIDE Gentamicin sulfate

N-Glycosidase A, PNGase A, N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase

N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum

PNGaseF from Elizabethkingia meningoseptica

PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, RECOMBINANT EXPRESSED IN ESCHERICHIA CO

PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, PROTEOMICS GRADE

peptide-N4-(N-acetyl--glucosaMinyl) asparagineaMidase

Glycopeptidase F from Elizabethkingia meningoseptica

PNGase F from Elizabethkingia miricola

N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase

EC 3.5.1.52

GLYCOPEPTIDASE

GLYCOPEPTIDASE A

GLYCOPEPTIDASE F

N-LINKED-GLYCOPEPTIDE-[N-ACETYL-BETA-D-GLUCOSAMINYL]-L-ASPARAGINE AMIDOHYDROLASE

N-GLYCANASE

N-GLYCANASE(R)

N-GLYCOSIDASE A

N-GLYCOSIDASE A, ALMOND

N-GLYCOSIDASE F

N-GLYCOSIDASE F, CHRYSEOBACTERIUM MENINGOSEPTICUM

PNGASE A

PNGASE F

PNGASE (F CHRYSEOBACTERIUM (FLAVOBACTERIUM) MENINGOSEPTICUM)

PEPTIDE-N4-(ACETYL-BETA-GLUCOSAMINYL)-ASPARAGINE AMIDASE

(PEPTIDE-N4-[N-ACETYL-BETA-GLUCOSAMINYL)]

PEPTIDE N-GLYCOSIDASE

PEPTIDE-N-GLYCOSIDASE F

PNGASE F HIGH CONCENTRATION FROM CHRYSEO

PNGASE F RECOMBINANT CHRYSEOBACTERIUM

GLYCOPEPTIDASE FROM ALMONDS

glycopeptidase a from almonds

pngase f from elizabethkingia (chryseobacterium/flavobacterium) meningsepticum

GLYCOPEPTIDASE F, RECOMBINANT, SOLUTION, FROM FLAVOBACTERIUM MENINGOSEPTICUM

N-Glycanase, N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum

N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli

N-Glycosidase F, Elizabethkingia meningosepticum

Native Almonds Glycopeptidase A

Native Elizabethkingia meningoseptica PNGase F

PNGase F from Elizabethkingia miricola, Recombinant

PNGase F from Flavobacterium meningosepticum, Recombinant

Native Elizabethkingia miricola PNGase F

Native Flavobacterium meningosepticum PNGase F

PNGase F from Elizabethkingia meningoseptica, Recombinant

peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase

buffered aqueous solution, recombinant, expressed in E. coli

PNGase F from Elizabethkingia meningoseptica lyophilized powder, recombinant, expressed in E. coli

Glycopeptidase A from almonds buffered aqueous glycerol solution, >=0.05 unit/mL

PNGase F from Elizabethkingia miricola buffered aqueous solution

PNGase F from Elizabethkingia meningoseptica BioReagent, >=95% (SDS-PAGE), for proteomics

PNGase F from Elizabethkingia meningoseptica buffered aqueous solution, recombinant, expressed in E. coli

PNGase F（Fast）

83534-39-8

Post-translational Modification

Proteomics and Protein Expression

Proteomics

Enzymes for Protein Carbohydrate Modification Analysis

Endoglycosidases

Enzymes, Inhibitors, and Substrates