PBR322 PLASMID DNA

Product Name: PBR322 PLASMID DNA
CAS No.: 93384-17-9
Chemical Name: PBR322 PLASMID DNA
Synonyms: PBR322 DNA;DNA, PBR322;PBR322 VECTOR;PBR322 PLASMID DNA;PBR325 PLASMID DNA;PLASMID PBR322 DNA;PLASMID PBR322 DEOXYRIBONUCLEIC ACID;Deoxyribonucleic acids, plasmid pBR322;pBR322 Plasmid DNA from E. coli RRI;DEOXYRIBONUCLEIC ACID, PLASMID (PBR 322 PLASMID)
CBNumber: CB3353064
Formula Weight: 0
MOL File: Mol file

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PBR322 PLASMID DNA Property

storage temp.: -20°C
form: lyophilized powder

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Safety

WGK Germany: 3
F: 3-10-21

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: D9893
Product name: pBR322 Plasmid DNA from E. coli RRI
Purity: lyophilized powder
Packaging: 1unit
Price: $186.8
Updated: 2024/03/01

Sigma-Aldrich

Product number: D4904
Product name: pBR322 Plasmid DNA from E. coli RRI
Purity: buffered aqueous solution
Packaging: 0.25unit
Price: $156
Updated: 2024/03/01

Sigma-Aldrich

Product number: D9893
Product name: pBR322 Plasmid DNA from E. coli RRI
Purity: lyophilized powder
Packaging: 5units
Price: $1870
Updated: 2024/03/01

Sigma-Aldrich

Product number: D4904
Product name: pBR322 Plasmid DNA from E. coli RRI
Purity: buffered aqueous solution
Packaging: 1unit
Price: $498
Updated: 2024/03/01

Sigma-Aldrich

Product number: D4904
Product name: pBR322 Plasmid DNA from E. coli RRI
Purity: buffered aqueous solution
Packaging: 5units
Price: $1740
Updated: 2024/03/01

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PBR322 PLASMID DNA Chemical Properties,Usage,Production

Uses

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.

Uses

pBR322 Plasmid DNA from?E. coli?RRI has been used to study about ferrous ion-induced strand breaks in the plasmid pBR322 are mediated through hydrogen peroxide.

Uses

Select for recombinant DNA. The Pst I and Pvu I cleavage sites in the ampicillin gene, or the Cla I, Hind III, BamH I, and Sal I sites in the tetracycline gene allow the insertion of foreign DNA fragments, and inactivation of one of these genes. pBR322 DNA has been used in to study artificial metallonuclease activity.

General Description

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.

The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).

Biochem/physiol Actions

This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.

PBR322 PLASMID DNA Preparation Products And Raw materials

Raw materials

Preparation Products

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PBR322 PLASMID DNA Suppliers

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93384-17-9, PBR322 PLASMID DNARelated Search:

SALCOMINE Tosylmethyl isocyanide METHYL ISOCYANOACETATE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) COBALT(II) ACETYLACETONATE 2,4-PENTANEDIONE, SILVER DERIVATIVE N-BUTYLISOCYANIDE PHENYLSELENOL 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Cupric acetylacetonate Ethyl isocyanoacetate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Aluminum acetylacetonate TERT-BUTYL ISOCYANIDE Benzyl isocyanide Ferric acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III)

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93384-17-9