PBR322 PLASMID DNA
- Product Name
- PBR322 PLASMID DNA
- CAS No.
- 93384-17-9
- Chemical Name
- PBR322 PLASMID DNA
- Synonyms
- PBR322 DNA;DNA, PBR322;PBR322 VECTOR;PBR322 PLASMID DNA;PBR325 PLASMID DNA;PLASMID PBR322 DNA;PLASMID PBR322 DEOXYRIBONUCLEIC ACID;Deoxyribonucleic acids, plasmid pBR322;pBR322 Plasmid DNA from E. coli RRI;DEOXYRIBONUCLEIC ACID, PLASMID (PBR 322 PLASMID)
- CBNumber
- CB3353064
- Formula Weight
- 0
- MOL File
- Mol file
PBR322 PLASMID DNA Property
- storage temp.
- -20°C
- form
- lyophilized powder
Safety
- WGK Germany
- 3
- F
- 3-10-21
N-Bromosuccinimide Price
- Product number
- D9893
- Product name
- pBR322 Plasmid DNA from E. coli RRI
- Purity
- lyophilized powder
- Packaging
- 1unit
- Price
- $186.8
- Updated
- 2024/03/01
- Product number
- D4904
- Product name
- pBR322 Plasmid DNA from E. coli RRI
- Purity
- buffered aqueous solution
- Packaging
- 0.25unit
- Price
- $156
- Updated
- 2024/03/01
- Product number
- D9893
- Product name
- pBR322 Plasmid DNA from E. coli RRI
- Purity
- lyophilized powder
- Packaging
- 5units
- Price
- $1870
- Updated
- 2024/03/01
- Product number
- D4904
- Product name
- pBR322 Plasmid DNA from E. coli RRI
- Purity
- buffered aqueous solution
- Packaging
- 1unit
- Price
- $498
- Updated
- 2024/03/01
- Product number
- D4904
- Product name
- pBR322 Plasmid DNA from E. coli RRI
- Purity
- buffered aqueous solution
- Packaging
- 5units
- Price
- $1740
- Updated
- 2024/03/01
PBR322 PLASMID DNA Chemical Properties,Usage,Production
Uses
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.
Uses
pBR322 Plasmid DNA from?E. coli?RRI has been used to study about ferrous ion-induced strand breaks in the plasmid pBR322 are mediated through hydrogen peroxide.
Uses
Select for recombinant DNA. The Pst I and Pvu I cleavage sites in the ampicillin gene, or the Cla I, Hind III, BamH I, and Sal I sites in the tetracycline gene allow the insertion of foreign DNA fragments, and inactivation of one of these genes. pBR322 DNA has been used in to study artificial metallonuclease activity.
General Description
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
Biochem/physiol Actions
This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.
PBR322 PLASMID DNA Preparation Products And Raw materials
Raw materials
Preparation Products
PBR322 PLASMID DNA Suppliers
- Tel
- --
- Fax
- --
- info-tech@nacalai.co.jp
- Country
- Japan
- ProdList
- 6046
- Advantage
- 75