Aminoacylase
- Product Name
- Aminoacylase
- CAS No.
- 9012-37-7
- Chemical Name
- Aminoacylase
- Synonyms
- ACYLASE;ACYLASE I;L-AMINOACYLASE;ACYLASE 1;histozyme;hippurase;acyclase I;EC 3.5.1.14;hippuricase;benzamidase
- CBNumber
- CB3458624
- Molecular Formula
- C30H34Cl2N4O
- Formula Weight
- 537.52316
- MOL File
- 9012-37-7.mol
Aminoacylase Property
- storage temp.
- 2-8°C
- form
- salt-free, lyophilized powder
- color
- yellow-brown
- EPA Substance Registry System
- Aminoacylase (9012-37-7)
Safety
- Hazard Codes
- Xn
- Risk Statements
- 36/37/38-42
- Safety Statements
- 22-24/25-36/37-26-24
- WGK Germany
- 3
- RTECS
- BF4890000
- F
- 3-10-21
- HS Code
- 35079090
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H334May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P271Use only outdoors or in a well-ventilated area.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- 01818
- Product name
- Acylase I from Aspergillus melleus
- Purity
- powder, brown, >0.5 U/mg
- Packaging
- 5g
- Price
- $47.2
- Updated
- 2024/03/01
- Product number
- 01818
- Product name
- Acylase I from Aspergillus melleus
- Purity
- powder, brown, >0.5 U/mg
- Packaging
- 25g
- Price
- $171
- Updated
- 2024/03/01
- Product number
- 01824
- Product name
- Acylase I, immobilized on Eupergit® C from Aspergillus sp.
- Purity
- ≥50?U/g moist material
- Packaging
- 1G
- Price
- $126
- Updated
- 2023/01/07
- Product number
- A0688
- Product name
- Acylase from Aspergillus genus
- Packaging
- 25g
- Price
- $147
- Updated
- 2024/03/01
- Product number
- A8376
- Product name
- Acylase I from porcine kidney
- Purity
- Grade II, salt-free, lyophilized powder, 500-1,500units/mg protein
- Packaging
- 1g
- Price
- $230
- Updated
- 2024/03/01
Aminoacylase Chemical Properties,Usage,Production
Description
Aminoacylase-1 (EC 3.5.1.14) is a homodimeric zinc-binding metalloenzyme. A cytosolic enzyme with a wide range of tissue expression, it cleaves acylated L-amino acids (except L-aspartate) into L-amino acids and an acyl group. L-aspartate derivatives are cleaved by aminoacylase-2 (aspartoacylase). Aminoacylase-1 is the most abundant of the aminoacylases, a class of enzymes involved in hydrolysis of N-acetylated proteins.
Uses
Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .
Application
Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids.
Biological Functions
Aminoacylases (N-acyl-L-amino acid amidohydrolases; EC 3.5.1.14) are widely found in animals, plants and microorganisms. The primary function of these enzymes is to remove acyl residues from N-acetylated amino acids although they may also be capable of hydrolysing carboxylic acid amides to fatty acid anions and L-amino acids. Although the catalytic mechanism of aminoacylases has been known for decades, the physiological role of these enzymes is still poorly understood. Activities of a similar nature, however, have been found in certain carboxypeptidases, aminopeptidases and dipeptidases. It could be therefore suggested that aminoacylases have a role to play in protein/peptide turnover.
General Description
Acylase I belongs to the aminoacylase family of enzymes.
Biochem/physiol Actions
Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .
Enzyme inhibitor
Aminoacylase is a metallo-enzyme that needs Zinc (Zn2+) as a cofactor to function. The Zinc ions inside of aminoacylase are each coordinated to histidine, glutamate, aspartate, and water. The Zinc ion polarizes the water, facilitating its deprotonation by a nearby basic residue. The negatively charged hydroxide ion is nucleophilic and attacks the electrophilic carbonyl carbon of the substrate's acyl group.The exact mechanism after this point is unknown, with one possibility being that the carbonyl then reforms, breaks the amide bond, and forms the two products. At some point in the mechanism, another water molecule enters and coordinates with Zinc, returning the enzyme to its original state.
The nucleophilic attack by water is the rate-limiting step of aminoacylase's catalytic mechanism. This nucleophilic attack is reversible while the subsequent steps are fast and irreversible. This reaction sequence is an example of Michaelis–Menten kinetics, allowing one to determine KM, Kcat, Vmax, turnover number, and substrate specificity through classic Michaelis-Menten enzyme experiments. The second and third forward steps cause the formation and release of the reaction's products.
Aminoacylase Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Aminoacylase manufacturers
- Product
- AMINOACYLASE 9012-37-7
- Price
- US $8.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 10tons
- Release date
- 2018-12-25