α-Chymotrypsin

Degradation

Product Name: α-Chymotrypsin
CAS No.: 9004-07-3
Chemical Name: α-Chymotrypsin
Synonyms: α-Chymotrypsin;A-CHYMOTRYPSIN;EC 3.4.21.1;ALPHA-CHYMOTRYPSIN;zolyse;zolyes;D03484;chymar;ec3445;ec3446
CBNumber: CB4167554
Molecular Formula: N/A
Formula Weight: 0
MOL File: Mol file

More

Less

α-Chymotrypsin Property

Melting point:: 127°C
Density: 1.37[at 20℃]
vapor pressure: 0Pa at 25℃
storage temp.: -20°C
solubility: Reconstitute in 1mM HCl. Soluble at 10mg/ml in 1mM HCl. 2mM calcium chloride serves as a stabilizer. Store aliquoted solutions at -20°C for up to a week.
form: salt-free, lyophilized powder
color: white
Water Solubility: 125g/L at 25℃
Merck: 13,2282
LogP: -1.3 at 20℃
EPA Substance Registry System: Chymotrypsin (9004-07-3)

More

Less

Safety

Hazard Codes: Xn,B
Risk Statements: 36/37/38-42/43-42
Safety Statements: 26-36-36/37-24-22
WGK Germany: 3
RTECS: GC3050000
F: 3-10
TSCA: Yes
HS Code: 35079090
Hazardous Substances Data: 9004-07-3(Hazardous Substances Data)

More

Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H317May cause an allergic skin reaction

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

More

Less

N-Bromosuccinimide Price

Sigma-Aldrich

Product number: SRP6509
Product name: Chymotrypsin from human pancreas
Purity: ≥95% (SDS-PAGE)
Packaging: 100μg
Price: $428
Updated: 2024/03/01

Sigma-Aldrich

Product number: CHY5S
Product name: α-Chymotrypsin from bovine pancreas
Purity: ≥40?units/mgprotein,vialof5?mg
Packaging: 10vials
Price: $310
Updated: 2024/03/01

Sigma-Aldrich

Product number: C9134
Product name: α-Chymotrypsin−Agarose from bovine pancreas
Purity: lyophilized powder, 2,000-3,500?units/g agarose (One ml gel will yield 65-120?units)
Packaging: 50units
Price: $327
Updated: 2024/03/01

Sigma-Aldrich

Product number: C9134
Product name: α-Chymotrypsin−Agarose from bovine pancreas
Purity: lyophilized powder, 2,000-3,500?units/g agarose (One ml gel will yield 65-120?units)
Packaging: 100units
Price: $582
Updated: 2024/03/01

Sigma-Aldrich

Product number: C8946
Product name: α-Chymotrypsin from human pancreas
Purity: lyophilized powder
Packaging: 1VIAL
Price: $688
Updated: 2024/03/01

More

Less

α-Chymotrypsin Chemical Properties,Usage,Production

Degradation

The rate and extent of insulin degradation by trypsin and α-Chymotrypsin were examined in vitro, and the initial sites of cleavage by α-Chymotrypsin were identified. The apparent Km for both enzymes was approximately the same, but the apparent Vmax for α-Chymotrypsin was 8.6 times greater. At a molar ratio of 172:1 (insulin: enzyme), chymotrypsin caused near-total loss of insulin within 40 min, while very little insulin was degraded by trypsin. Chymotrypsin appeared to cleave initially at the carboxyl side of the B26-Tyr and A19-Tyr residues, and additional cleavage at the B16-Tyr, B25-Phe, and A14-Tyr residue sites also occurred rapidly. Only two to three other susceptible bonds, which are not exposed at the surface of the insulin molecule, remained intact after the quenching of initial cleavage[1].

Chemical Properties

Lyophilized powder, dialyzed

Uses

α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.

Uses

The enzyme from Sigma has been used to assess the effect of limited proteolysis with α-chymotrypsin on the sperm penetration.

Uses

α-Chymotrypsin from bovine pancreas has been used in a study to investigate protein extraction by Winsor-III microemulsion systems. α-Chymotrypsin from bovine pancreas has also been used in a study to investigate a new specific fullerene-based fluorescent probe for trypsin.

General Description

Chymotrypsin (Chymar) is extractedfrom mammalian pancreas and is used in cataractsurgery. A dilute solution is used to irrigate the posteriorchamber of the eye to dissolve the fine filaments that holdthe lens.

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+.

Mechanism of action

The general mechanism for chymotrypsin is the classic serine protease mechanism. Hydrolytic proteolysis by α-chymotrypsin begins with an initial nucleophilic attack on the peptide bond by Ser 195, activated by deprotonation by His 57. This leads to forming a tetrahedral intermediate, stabilized by the amide groups of Ser 195 and Gly 193. The subsequent collapse of this intermediate, assisted by protonation of the leaving group by His 57 and Asp 102, leads to an acyl-enzyme intermediate. Activation of a water molecule by His 57 and Asp 102 facilitates hydrolysis of this intermediate, resulting in the reformation of the catalytically active serine residue and the release of the product facilitated by protonation with His 57.

Purification Methods

α-Chymotrypsin is crystallised twice from four-tenths saturated ammonium sulfate solution, then dissolved in 1mM HCl and dialysed against 1mM HCl at 2-4o. The solution is stored at 2o [Lang et al. J Am Chem Soc 80 4923 1958].

References

[1] R J Schilling, A K Mitra. “Degradation of insulin by trypsin and alpha-chymotrypsin.” Pharmaceutical Research 8 6 (1991): 721–7.

α-Chymotrypsin Preparation Products And Raw materials

Raw materials

FUMING SULFURIC ACID Trypsin LACTIS PROTEINUM Ammonium sulfate Celite

Preparation Products

More

Less

α-Chymotrypsin Suppliers

Canada 2 China 238 Europe 3 France 1 Germany 7 India 13 Japan 4 Singapore 1 Switzerland 1 United Kingdom 6 United States 35 Global 311

HBCChem, Inc.

Tel: +1-510-219-6317
Fax: +1-650-486-1361
Email: sales@hbcchem.com
Country: United States
ProdList: 10648
Advantage: 60

TargetMol Chemicals Inc.

Tel: +1-781-999-5354 +1-00000000000
Email: marketing@targetmol.com
Country: United States
ProdList: 32165
Advantage: 58

Aladdin Scientific

Tel: +1-+1(833)-552-7181
Email: sales@aladdinsci.com
Country: United States
ProdList: 52925
Advantage: 58

Aladdin Scientific

Tel: +1-+1(833)-552-7181
Email: sales@aladdinsci.com
Country: United States
ProdList: 57505
Advantage: 58

United States Biological

Tel: --
Fax: --
Email: sales@advtechind.com
Country: United States
ProdList: 6075
Advantage: 58

Creative Enzymes

Tel: 1-516-855-7709
Fax: 1-631-938-8127
Email: info@creative-enzymes.com
Country: United States
ProdList: 8748
Advantage: 58

ProteoChem Inc.

Tel: --
Fax: --
Email: info@proteochem.com
Country: United States
ProdList: 74
Advantage: 38

AppliChem, LLC

Tel: --
Fax: --
Email: sales@applichem.com
Country: United States
ProdList: 169
Advantage: 50

Scandinavian Formulas

Tel: --
Fax: --
Email: mikep@scandinavianformulas.com
Country: United States
ProdList: 1550
Advantage: 58

United States Biological

Tel: --
Fax: --
Email: chemicals@usbio.net
Country: United States
ProdList: 6214
Advantage: 80

Creative Enzymes

Tel: --
Fax: --
Email: info@creative-enzymes.com
Country: United States
ProdList: 6057
Advantage: 58

Worthington Biochemical Corporation

Tel: --
Fax: --
Email: techservice@worthington-biochem.com
Country: United States
ProdList: 3
Advantage: 58

Sigma-Aldrich Corporation

Tel: --
Fax: --
Email: labwatersalessupport@emdgroup
Country: United States
ProdList: 2
Advantage: 58

Riedel-de Haen AG

Tel: --
Fax: --
Country: United States
ProdList: 6773
Advantage: 87

Sisco Research Laboratories Pvt. Ltd.

Tel: --
Fax: --
Email: srlmarketing@dishnetdsl.net
Country: United States
ProdList: 1905
Advantage: 64

Lee Biosolutions, Inc.

Tel: --
Fax: --
Email: info@leebio.com
Country: United States
ProdList: 72
Advantage: 50

HBCChem Inc.

Tel: --
Fax: --
Email: sales@hbcchem-inc.com
Country: United States
ProdList: 4569
Advantage: 30

Waterstone Technology, LLC

Tel: --
Fax: --
Email: sales@waterstonetech.com
Country: United States
ProdList: 6786
Advantage: 30

Chem-Impex International, Inc.

Tel: --
Fax: --
Country: United States
ProdList: 6730
Advantage: 64

AK Scientific, Inc.

Tel: --
Fax: --
Email: sales@aksci.com
Country: United States
ProdList: 6347
Advantage: 65

Sciencelab.com, Inc.

Tel: --
Fax: --
Email: accounting@sciencelab.com
Country: United States
ProdList: 4159
Advantage: 82

EMD Biosciences, Inc.

Tel: --
Fax: --
Email: technical@calbiochem.com
Country: United States
ProdList: 6529
Advantage: 66

Spectrum Chemicals & Laboratory Products

Tel: --
Fax: --
Email: sales@spectrumchemical.com
Country: United States
ProdList: 6699
Advantage: 77

Crescent Chemical Co., Inc.

Tel: --
Fax: --
Email: sales@creschem.com
Country: United States
ProdList: 4597
Advantage: 68

MP Biomedicals, Inc.

Tel: --
Fax: --
Country: United States
ProdList: 6561
Advantage: 81

Research Plus, Inc.

Tel: --
Fax: --
Email: respls@aol.com
Country: United States
ProdList: 3674
Advantage: 51

Pfaltz & Bauer, Inc.

Tel: --
Fax: --
Email: sales@pfaltzandbauer.com
Country: United States
ProdList: 6828
Advantage: 72

Roche Applied Science

Tel: --
Fax: --
Email: biochemts.us@roche.com
Country: United States
ProdList: 696
Advantage: 85

AMRESCO Inc.

Tel: --
Fax: --
Email: info@amresco-inc.com
Country: United States
ProdList: 760
Advantage: 73

Aceto Corporation

Tel: --
Fax: --
Email: contact@aceto.com
Country: United States
ProdList: 2619
Advantage: 75

BIODESIGN International/OEM Concepts

Tel: --
Fax: --
Email: info@biodesign.com
Country: United States
ProdList: 2376
Advantage: 50

Worthington Biochemical Corporation

Tel: --
Fax: --
Email: office@worthington-biochem.com
Country: United States
ProdList: 109
Advantage: 77

Karlan Research Products Corporation

Tel: --
Fax: --
Email: info@karlan.com
Country: United States
ProdList: 420
Advantage: 51

Research Organics Inc.

Tel: --
Fax: --
Email: info@resorg.com
Country: United States
ProdList: 1324
Advantage: 72

Scandinavian Formulas, Inc.

Tel: --
Fax: --
Email: info@scandinavianformulas.com
Country: United States
ProdList: 1447
Advantage: 55

More

Less

View Lastest Price from α-Chymotrypsin manufacturers

BINBO BIOLOGICAL CO.,LTD

Product: Chymotrypsin 9004-07-3
Price: US $0.00/kg
Min. Order: 1kg
Purity: 99.8%
Supply Ability: 1000 kg
Release date: 2024-03-28

R&D Scientific Inc.

Product: Chymotrypsin 9004-07-3
Price: US $4500.00/Kg
Min. Order: 1Kg
Purity: 97
Supply Ability: 500 Kg
Release date: 2024-06-27

Shaanxi TNJONE Pharmaceutical Co., Ltd

Product: Alpha Chymotrypsin 9004-07-3
Price: US $0.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 10000kg
Release date: 2024-05-13

9004-07-3, α-ChymotrypsinRelated Search:

Diethylstilbestrol Trypsin Proteinase K ENTEROKINASE Aprotinin CHYMOTRYPSINOGEN A 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Aluminum acetylacetonate Tosylmethyl isocyanide Cupric acetylacetonate Tris(2,4-pentanedionato)chroMiuM(III) N-BUTYLISOCYANIDE Benzyl isocyanide TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) TERT-BUTYL ISOCYANIDE Ferric acetylacetonate Ethyl isocyanoacetate SALCOMINE

quimotrase

zolyse

TLCK-CHYMOTRYPSIN

ALPHA-CHYMOTRYPSIN, TLCK TREATED

ALPHA-CHYMOTRYPSIN TYPE I-S

ALPHA-CHYMOTRYPSIN TYPE VI

ALPHA-CHYMOTRYPSIN TYPE VII

ALPHA-CHYMOTRYPSIN

ALPHA-CHYMOTRYPSIN BOVINE PANCREAS

BETA-CHYMOTRYPSIN

EC 3.4.21.1

EC: 3.4.21.1

CHYMOTRYPSIN, BOVINE PANCREAS

CHYMOTRYPSIN

CHYMOTRYPSIN A

CHYMOTRYPSIN, ALPHA

D-CHYMOTRYPSIN

IUB: 3.4.21.1

α-Chymotrypsin Insoluble enzyme attached to carboxymethyl cellulose from bovine pancreas

chymetin

zolyes

zonulsin

α-Chymotrypsin, Inactivated from bovine pancreas

α-Chymotrypsin-Agarose from bovine pancreas

Catarase (tn)

Chymotrypsin (jan/usp/inn)

D03484

Alpapsin

alpha-Chymotrypsin from Bovine Pancreas (3* recrystallized, salt free from 20% Ethanol, presence of Calcium enhances its activity and stability)

beta-Chymotrypsin from Bovine Pancreas (3* recrystallized, salt free from 20% Ethanol, presence of Calcium enhances its activity and stability)

beta-Chymotrypsin from Bovine Pancreas

Chymotrypsin from bovine pancreas min. 40 U/mg

Chymotrypsin (300 mg)

a-Chymotrypsin - from bovine pancreas, >45 units/MG protein

a-Chymotrypsin - USP 1000units/mg

alfapsin

alphachymar

alpha-chymarophth

alpha-chymotrypsinfrombovinepancreas

avazyme

catarase

chymar

chymolase

chymotest

chymotrypsinb

chymozym

e.c.3.4.4.5

e.c.3.4.4.6

ec3445

ec3446

enzeon

impral

kimopsin

kimoral

quimar

3xcryst.

α-chymotrypsin from human pancreas

α-chymotrypsin tlck treated from bovine pancreas