ChemicalBook > CAS DataBase List > 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Product Name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
CAS No.
6578-06-9
Chemical Name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Synonyms
BCIP;BClP;BCIP-T;abs9176;BCIP P-TOL;X-PHOS P-TOL;BCIP-TOLUIDINE;BCIP P-TOLUIDINE;BCIP/INT SUBSTRATE;BCIP p-toluidine sal
CBNumber
CB4854083
Molecular Formula
C8H6BrClNO4P.C7H9N
Formula Weight
433.63
MOL File
6578-06-9.mol
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Property

Melting point:
194-195℃
storage temp. 
-20°C
solubility 
DMF: 20 mg/mL
form 
tablets
color 
White to Orange to Green
InChIKey
QEIFSLUFHRCVQL-UHFFFAOYSA-N
CAS DataBase Reference
6578-06-9(CAS DataBase Reference)
EPA Substance Registry System
1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38-40
Safety Statements 
26
RIDADR 
2811
WGK Germany 
3
F 
3-8-10
HazardClass 
6.1
PackingGroup 
III
HS Code 
29339190
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H226Flammable liquid and vapour

H319Causes serious eye irritation

Precautionary statements

P201Obtain special instructions before use.

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P370+P378In case of fire: Use … for extinction.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
BCIPP-RO
Product name
BCIP
Purity
5-bromo-4-chloro-3-indolyl-phosphate,4-toluidinesalt
Packaging
250mg
Price
$208
Updated
2024/03/01
Sigma-Aldrich
Product number
BCIPP-RO
Product name
BCIP
Purity
5-bromo-4-chloro-3-indolyl-phosphate,4-toluidinesalt
Packaging
1g
Price
$453
Updated
2024/03/01
Sigma-Aldrich
Product number
B0274
Product name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Purity
tablet
Packaging
10tablets
Price
$399
Updated
2024/03/01
Sigma-Aldrich
Product number
B0274
Product name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Purity
tablet
Packaging
25tablets
Price
$794
Updated
2024/03/01
Sigma-Aldrich
Product number
B6777
Product name
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Purity
BioReagent, for molecular biology, powder, ≥99%
Packaging
100MG
Price
$121
Updated
2023/06/20
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Chemical Properties,Usage,Production

Chemical Properties

White to light brown powder

Uses

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

General Description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Preparation Products And Raw materials

Raw materials

Preparation Products

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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Suppliers

Americas 1 Austria 1 Canada 2 China 189 Europe 3 France 1 Germany 5 India 5 Japan 4 South Korea 1 Switzerland 3 United Kingdom 11 United States 35 Global 261
Suzhou Rosen Biotechnology Co.,Ltd.
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0512-87888687 13626191410
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0512-62929131
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rosenchemical@163.com
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China
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Shanghai JONLN Reagent Co., Ltd.
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400-0066400 13621662912
Fax
021-55660885
Email
422131432@qq.com
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China
ProdList
9978
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Jiangsu Hi-Pure Technology New Materials Co. Ltd.
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0512-62764072; 18051857768
Email
sales@hi-pure.cn
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China
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Guangzhou Yaoguang Technology Co., Ltd.
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13020159086
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yangjue@ygfuture.com
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China
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J & K SCIENTIFIC LTD.
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86-10-82849933
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jkinfo@jkchemical.com
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Meryer (Shanghai) Chemical Technology Co., Ltd.
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021-61259108 18621169109
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86-21-61259102
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market03@meryer.com
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China
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40228
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INTATRADE GmbH
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+49 3493/605464
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+49 3493/605470
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sales@intatrade.de
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Germany
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future industrial shanghai co., ltd
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400-0066400 13621662912
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021-55660885
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sales@jonln.com
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China
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TCI (Shanghai) Development Co., Ltd.
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021-67121386
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021-67121385
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Sales-CN@TCIchemicals.com
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China
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Energy Chemical
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021-021-58432009 400-005-6266
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021-58436166
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sales8178@energy-chemical.com
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View Lastest Price from 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt 6578-06-9
Price
US $0.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
3500kg/month
Release date
2021-09-14
Henan Fengda Chemical Co., Ltd
Product
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt 6578-06-9
Price
US $10.00-1.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-08
Zhuozhou Wenxi import and Export Co., Ltd
Product
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt 6578-06-9
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-10

6578-06-9, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine saltRelated Search:

AMPHISOL A BCDMH 4-Methylaniline hydrochloride Trisodium phosphate Calcium phosphate Chlorobromoisocyanurate Sodium Phosphate Monobasic Monohydrate p-Toluidine CHLORODIBROMOMETHANE Tributyl phosphate phosphate 8-CHLOROADENOSINE-3',5'-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Bromine BCIP,BCIP 2 K,BCIP DIPOTASSIUM SALT 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALT X-PHOS BCIP/NBT toluidine

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  • Bcip,P-ToluidineSalt(5-Bromo-4-Chloro-3-IndoylPhosphate)
  • BCIP 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE
  • 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1)
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT MOLECULAR BIOLOGY REAGENT 98+% BCIP
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