Dansyl chloride Property

Melting point:

72-74 °C(lit.)

Boiling point:

371.3±22.0 °C(Predicted)

Density 

1.1702 (rough estimate)

refractive index 

1.5630 (estimate)

storage temp. 

2-8°C

solubility 

acetone: 50 mg/mL

form 

powder and chunks

pka

2.38±0.40(Predicted)

color 

yellow to orange

Water Solubility 

Soluble in dimethyl fluoride, acetone, chloroform, pyridine, benzene and dioxane. Insoluble in water.

Sensitive 

Moisture Sensitive

Merck 

14,2814

BRN 

2217205

Stability:

Hygroscopic, Moisture Sensitive

InChIKey

XPDXVDYUQZHFPV-UHFFFAOYSA-N

CAS DataBase Reference

605-65-2(CAS DataBase Reference)

EPA Substance Registry System

1-Naphthalenesulfonyl chloride, 5-(dimethylamino)- (605-65-2)

Safety

Hazard Codes 

C

Risk Statements 

34

Safety Statements 

26-36/37/39-45-3

RIDADR 

UN 3261 8/PG 2

WGK Germany 

3

RTECS 

QK3688000

F 

10-21

TSCA 

Yes

HazardClass 

8

PackingGroup 

III

HS Code 

29214980

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H290May be corrosive to metals

H314Causes severe skin burns and eye damage

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

Dansyl chloride Chemical Properties,Usage,Production

Uses

Dansyl chloride or 5-Dimethylaminonaphthalene-1-sulfonyl chloride (CAS 605-65-2) is a fluorogenic reagent used for protein, N-terminal amino acid and peptide detection by reverse phase high performance liquid chromatography (HPLC). It is also used to determine the degree of labeling in chymotrypsin and ovalbumin. It acts as a precursor for the synthesis of other derivatives like dansyl amide.
Dansyl chloride is non-fluorescent until it reacts with amines. The resulting Dansyl amides have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl chloride an important tool for biophysical studies.

Chemical Properties

yellow to orange-yellow crystalline powder

Uses

A fluorogenic reagent for N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC.

Uses

Dansyl chloride is a fluorogenic reagent used for protein, N-terminal amino acid and peptide detection by reverse phase high performance liquid chromatography (HPLC). It is also used to determine the degree of labeling in chymotrypsin and ovalbumin. It acts as a precursor for the synthesis of other derivatives like dansyl amide.

Uses

Reagent for fluorescent labelling of amines, amino acids and proteins.

Uses

In fluorescent labelling, column chromatography, HPLC.

Definition

ChEBI: Dansyl chloride is an aminonaphthalene and a sulfonic acid derivative.

General Description

Dansyl chloride is a fluorogenic reagent which is typically employed for the derivatization of amino acids.

Biochem/physiol Actions

Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins. Dansyl chloride is used for derivatization of free amino acids and amines in neural samples and biological fluids. It is used to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization.

Safety Profile

Poison by intravenous route, When heated to decomposition it emits very toxic fumes of Cland NOx