Iodonitrotetrazolium chloride

ChemicalBook > CAS DataBase List > Iodonitrotetrazolium chloride

Iodonitrotetrazolium chloride

Product Name: Iodonitrotetrazolium chloride
CAS No.: 146-68-9
Chemical Name: Iodonitrotetrazolium chloride
Synonyms: INT;INT DYE;IntA.R.;INT CHLORIDE;2-(p-iodophe;I.N.T. 95% AR;IODONITROTETRAZOLIUM;IODONITROTETRAZOLIUM VIOLET;Iodonitrotetrazolium purple;IODONITRO TETRAZOLIUM (INT)
CBNumber: CB7232772
Molecular Formula: C19H13ClIN5O2
Formula Weight: 505.7
MOL File: 146-68-9.mol

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Iodonitrotetrazolium chloride Property

Melting point:: 240 °C (dec.)(lit.)
storage temp.: 2-8°C
solubility: methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow
form: Powder, Crystals and/or Chunks
color: White to off-white
Water Solubility: soluble
λmax: 248nm
Sensitive: Light Sensitive
BRN: 4093224
Stability:: Stable. Incompatible with strong oxidizing agents.
Biological Applications: Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer
InChI: InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChIKey: JORABGDXCIBAFL-UHFFFAOYSA-M
SMILES: [N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-]
CAS DataBase Reference: 146-68-9(CAS DataBase Reference)
EPA Substance Registry System: 2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9)

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Safety

Hazard Codes: Xn,F,Xi
Risk Statements: 20/21/22-68/20/21/22-36/37/38-11
Safety Statements: 36/37-36/37/39-26-22-16
RIDADR: 2811
WGK Germany: 3
F: 3-8-10
TSCA: Yes
HazardClass: 6.1
PackingGroup: III
HS Code: 29339900

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H228Flammable solid

H335May cause respiratory irritation

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P240Ground/bond container and receiving equipment.

P241Use explosion-proof electrical/ventilating/lighting/…/equipment.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 58030
Product name: Iodonitrotetrazolium chloride
Purity: BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging: 1g
Price: $218
Updated: 2024/03/01

Sigma-Aldrich

Product number: 58030
Product name: Iodonitrotetrazolium chloride
Purity: BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging: 5G
Price: $763
Updated: 2024/03/01

Sigma-Aldrich

Product number: 58030
Product name: Iodonitrotetrazolium chloride
Purity: BioReagent, ≥97.0% (calc. on dry substance, NT)
Packaging: 25g
Price: $2560
Updated: 2024/03/01

TCI Chemical

Product number: I0067
Product name: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride
Purity: >98.0%(HPLC)(T)
Packaging: 1g
Price: $60
Updated: 2024/03/01

TCI Chemical

Product number: I0067
Product name: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride
Purity: >98.0%(HPLC)(T)
Packaging: 5g
Price: $189
Updated: 2024/03/01

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Iodonitrotetrazolium chloride Chemical Properties,Usage,Production

Description

Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.

Chemical Properties

slightly yellow powder

Uses

Electron acceptor for the colorimetric assay of various dehydrogenases.

Uses

Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator. Hence INT can be used for the colorimetric assay of various dehydrogenases.

Uses

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Definition

ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.

Safety

Iodonitrotetrazolium chloride could cause skin irritation, serious eye irritation, and may cause respiratory irritation.

Properties and Applications

INT (Iodonitrotetrazolium chloride), similar to tetrazolium chloride on reduction, produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine protein concentration in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.

Purification Methods

Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]

Iodonitrotetrazolium chloride Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Iodonitrotetrazolium chloride manufacturers

Career Henan Chemical Co

Product: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride 146-68-9
Price: US $100.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20kg
Release date: 2018-07-25

Career Henan Chemical Co

Product: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride 146-68-9
Price: US $100.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20kg
Release date: 2018-07-25

146-68-9, Iodonitrotetrazolium chlorideRelated Search:

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INT CHLORIDE

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IODONITROTETRAZOLIUM VIOLET

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Iodonitrotetrazolium chloride ,99%

IODONITROTETRAZOLIUM CHLORIDERESEARCH GRADE

Iodonitrotetrazolium chloride,2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

IntA.R.

2(4-IODOPHENYL)-3(4-NITROPHENYL)-5-PHENYL-2H-Tetrazolium Chloride (INT)extrapure AR

p-Iodonitrotetrazolium Violet, INT, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride

Iodonitrotetrazolium purple

INT, Iodonitrotetrazolium violet

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride hydrate,99%

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride [for Biochemical Research]

2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-3H-tetrazol-2-ium chloride

IODONITROTETRAZOLIUM CHLORIDE (INT DYE)

Iodonitrotetrazolium chloride p.a.

INT, 4-Iodonitrotetrazolium violet

INT Iodonitrotetrazolium Chloride p-Iodonitrotetrazolium Violet

2h-tetrazolium, 2-(piodophenyl)-3-(p-nitrophenyl)-5-phenyl-, Chloride

2-(p-iodophe

2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium:chloride

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazol-3-ium chloride

IODONITRO TETRAZOLIUM (INT)

Iodonitrotetrazolium chloride Ve

P-IODONITROTETRAZOLIUM VIOLET

2H-TETRAZOLIUM, 2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5- PHENYL-, CHLORIDE

2-(P-IODOPHENYL)-3-(P-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE

2-(P-IODOPHENYL)-3-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE

2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE

2-(4'-IODOPHENYL)-3-(4''-NITROPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE

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IODONITROTETRAZOLIUM

IODONITROTETRAZOLIUM CHLORIDE

2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2h-tetrazoliuchloride

INT Iodonitrotetrazolium chloride

2-(p-Iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium c

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazoliumChloride[forBiochemicalResearch]&gt

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazoliumChloride&gt

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride(INT) ,≥98%

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium chloride

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chlor...

Iodonitrotetrazolium chloride, for cell culture, 98%+

Iodonitrotetrazolium chloride, for cell culture+

lodonitrotetrazolium chloride

odonitrotetrazolium chloride

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride(INT)

I.N.T. 95% AR

146-68-9