PERTUSSIS TOXIN
- Product Name
- PERTUSSIS TOXIN
- CAS No.
- 70323-44-3
- Chemical Name
- PERTUSSIS TOXIN
- Synonyms
- PTX;iap;HOLOTOXIN;IAG2;ALPI;MAGT1;PSEC0084;pertussigen;UNQ628/PRO1244;pertussistoxins
- CBNumber
- CB8145592
- Molecular Formula
- NULL
- Formula Weight
- 0
- MOL File
- Mol file
PERTUSSIS TOXIN Property
- storage temp.
- 2-8°C
- solubility
- Water: soluble
- form
- lyophilized powder
Safety
- Hazard Codes
- T,B,Xi
- Risk Statements
- 23/24/25-36/37/38
- Safety Statements
- 36/37/39-45-26
- RIDADR
- UN 3462 6.1/PG 1
- WGK Germany
- 3
- RTECS
- XW5883750
- F
- 10
- HazardClass
- 6.1(a)
- PackingGroup
- I
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- P7208
- Product name
- Pertussis toxin from Bordetella pertussis
- Purity
- lyophilized powder
- Packaging
- 50μg
- Price
- $592
- Updated
- 2024/03/01
- Product number
- P2980
- Product name
- Pertussis toxin from Bordetella pertussis
- Purity
- buffered aqueous glycerol solution
- Packaging
- 50μg
- Price
- $628
- Updated
- 2024/03/01
- Product number
- P2980
- Product name
- Pertussis toxin from Bordetella pertussis
- Purity
- buffered aqueous glycerol solution
- Packaging
- 0.2mg
- Price
- $2100
- Updated
- 2024/03/01
- Product number
- 516561
- Product name
- Pertussis Toxin, Bordetella pertussis, Glycerol Solution - CAS 70323-44-3 - Calbiochem
- Purity
- Pertussis Toxin, Bordetella pertussis, Glycerol Solution, CAS 70323-44-3, is a protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins.
- Packaging
- 50μG
- Price
- $489
- Updated
- 2024/03/01
- Product number
- 516560
- Product name
- Pertussis Toxin, Bordetella pertussis - CAS 70323-44-3 - Calbiochem
- Purity
- This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.
- Packaging
- 50μG
- Price
- $489
- Updated
- 2024/03/01
PERTUSSIS TOXIN Chemical Properties,Usage,Production
Uses
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
General Description
Product is not activated. If activation is required, see Kaslow, et al., for suggested conditions.
Biochem/physiol Actions
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
storage
Store at +4°C
Purification Methods
Purify the toxin by stepwise elution from 3 columns comprising Blue Sepharose, Phenyl Sepharose and hydroxylapatite, and the purity is checked by SDS-PAGE. [Svoboda et al. Anal Biochem 159 402 1986, Biochemistry 21 5516 1982, Biochem J 83 295 1978.]