EC 3.4.22.1
- Product Name
- EC 3.4.22.1
- CAS No.
- 9047-22-7
- Chemical Name
- EC 3.4.22.1
- Synonyms
- CATHEPSIN B;EC 3.4.22.1;Cathepsin II;units/mg protein;Native Human Cathepsin B;Native Bovine Cathepsin B;CATHEPSIN B, BOVINE SPLEEN;CATHEPSIN B1, BOVINE SPLEEN;CATHEPSIN B FROM HUMAN LIVER;CATHEPSIN B FROM BOVINE SPLEEN
- CBNumber
- CB8206132
- Molecular Formula
- NULL
- Formula Weight
- 0
- MOL File
- Mol file
EC 3.4.22.1 Property
- storage temp.
- -70°C
- solubility
- H2O: soluble1.0mg/mL, clear
- form
- lyophilized powder
- color
- White to off-white
N-Bromosuccinimide Price
- Product number
- C6286
- Product name
- Cathepsin B from bovine spleen
- Purity
- lyophilized powder, ≥10?units/mg protein
- Packaging
- 10units
- Price
- $203
- Updated
- 2024/03/01
- Product number
- C6286
- Product name
- Cathepsin B from bovine spleen
- Purity
- lyophilized powder, ≥10?units/mg protein
- Packaging
- 25units
- Price
- $403
- Updated
- 2024/03/01
- Product number
- C8571
- Product name
- Cathepsin B from human liver
- Purity
- buffered aqueous solution, ≥1,500?units/mg protein (E1%/280)
- Packaging
- 25μg
- Price
- $468
- Updated
- 2024/03/01
- Product number
- C6286
- Product name
- Cathepsin B from bovine spleen
- Purity
- lyophilized powder, ≥10?units/mg protein
- Packaging
- 100units
- Price
- $1200
- Updated
- 2024/03/01
- Product number
- C0150
- Product name
- Cathepsin B from human placenta
- Purity
- lyophilized powder, ≥5?units/mg protein
- Packaging
- 2units
- Price
- $86.8
- Updated
- 2024/03/01
EC 3.4.22.1 Chemical Properties,Usage,Production
Uses
Cathepsin B from human liver has been used:
- as a positive control in the screening Cathepsin B inhibitor assay
- in wall extension (expansin activity) assay
- in microinjection human foreskin fibroblasts to test its effect on apoptosis induction
Uses
Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.
General Description
Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.
Biochem/physiol Actions
Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption.
Purification Methods
Cathepsin B is purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal-linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulfide [Rich et al. Biochem J 235 731 1986, Methods Enzymol 80 551 1981].
EC 3.4.22.1 Preparation Products And Raw materials
Raw materials
Preparation Products
EC 3.4.22.1 Suppliers
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