GLYCEROKINASE

Product Name: GLYCEROKINASE
CAS No.: 9030-66-4
Chemical Name: GLYCEROKINASE
Synonyms: GK;GLK;HK4;HHF3;HKIV;EC 2.7.1.30;GLYCEROKINASE;Glyceric kinase;Glycerin kinase;GLYCEROL KINASE
CBNumber: CB8437530
Molecular Formula: NULL
Formula Weight: 0
MOL File: Mol file

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GLYCEROKINASE Property

storage temp.: 2-8°C
form: lyophilized powder
color: White to off-white
EPA Substance Registry System: Kinase (phosphorylating), glycerol (9030-66-4)

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Safety

Safety Statements: 24/25
WGK Germany: 3
HS Code: 35079090

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P285In case of inadequate ventilation wear respiratory protection.

P304+P341IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: WH0002645M1
Product name: Monoclonal Anti-GCK antibody produced in mouse
Purity: clone 3C7, purified immunoglobulin, buffered aqueous solution
Packaging: 100μG
Price: $524
Updated: 2024/03/01

Sigma-Aldrich

Product number: G6278
Product name: Glycerokinase from Escherichia coli
Purity: 300-600?units/mL, ammonium sulfate suspension
Packaging: 200units
Price: $237
Updated: 2024/03/01

Sigma-Aldrich

Product number: G6142
Product name: Glycerokinase from Cellulomonas sp.
Purity: lyophilized powder, 25-75?units/mg protein
Packaging: 1000units
Price: $356
Updated: 2024/03/01

Sigma-Aldrich

Product number: G4509
Product name: Glycerokinase from Escherichia coli
Purity: lyophilized powder, 40-100?units/mg protein
Packaging: 1000units
Price: $690
Updated: 2024/03/01

Sigma-Aldrich

Product number: G0774
Product name: Glycerokinase from Bacillus stearothermophilus
Purity: buffered aqueous solution, ≥75?units/mg protein (biuret)
Packaging: 500units
Price: $537
Updated: 2024/03/01

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GLYCEROKINASE Chemical Properties,Usage,Production

Chemical Properties

Beige powder

Uses

Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.

Uses

This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.

Uses

Glycerol kinase has been used along with adenosine triphosphate (ATP) for the conversion of glycerol to glycerol-3 phosphate (glycerol-3P) in white adipose tissue (WAT) adipocyte samples by label distribution method. It has also been used along with adenosine triphosphate (ATP) in the derivatization of glycerol to sn-glycerol-3 phosphate (glycerol-3P).

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Purification Methods

Commercial enzyme has been dialysed against 2mM Hepes, 5mM dithiothreitol and 0.3mM EDTA, followed by several changes of 20mM Hepes and 5mM dithiothreitol prior to storage under N2 at -20o. [Knight & Cleland Biochemistry 28 5728 1989.] The enzyme from pigeon liver is purified by acid-precipitation (acetate buffer at pH 5.1), (NH4)2SO4 fractionation, heat treatment (60o/ 1hour), calcium phosphate gel filtration, a second (NH4)2SO4 fractionation, dialysis, elution of inert proteins and crystallisation. This is done by repeatedly extracting the precipitate from the last step with 0.05M sodium pyrophosphate (pH 7.5) containing 1mM EDTA and 0.2M (NH4)2SO4 is added. Careful addition of solid (NH4)2SO4 to this solution leads to crystallisation of the enzyme. Recrystallisation is repeated. The enzyme is activated by Mg2+ and Mn2+ ions and is most stable in solutions in the pH 4.5-5.5 range. The stability is greatly increased in the presence of glycerol. It has Km for glycerol of 60WM and for ATP 9WM in glycine buffer pH 9.8 and 25o. [Kennedy Methods Enzymol 5 476 1962.]

GLYCEROKINASE Preparation Products And Raw materials

Raw materials

Preparation Products

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GLYCEROKINASE Suppliers

China 144 Europe 2 Germany 2 India 3 Japan 1 Switzerland 1 United States 10 Global 163

Creative Enzymes

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View Lastest Price from GLYCEROKINASE manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Glycerokinase 9030-66-4
Price: US $0.00/KU
Min. Order: 10KU
Purity: ≥90%
Supply Ability: In stock
Release date: 2021-05-28

Hubei Xindesheng Material Technology Co., Ltd.

Product: Glycerin kinase 9030-66-4
Price: US $10.50-7.50/KU
Min. Order: 10KU
Purity: ≥ 20U/ mg
Supply Ability: 10000ku
Release date: 2020-11-04

Career Henan Chemical Co

Product: GLYCEROKINASE 9030-66-4
Price: US $1.00/KG
Min. Order: 1KG
Purity: 95%-99%
Supply Ability: 1000kg
Release date: 2018-12-24

9030-66-4, GLYCEROKINASERelated Search:

PHENYLSELENOL Ferric acetylacetonate TERT-BUTYL ISOCYANIDE METHYL ISOCYANOACETATE COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Tosylmethyl isocyanide 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE 2,4-PENTANEDIONE, SILVER DERIVATIVE Ethyl isocyanoacetate Tris(2,4-pentanedionato)chroMiuM(III) SALCOMINE Benzyl isocyanide TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Aluminum acetylacetonate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Cupric acetylacetonate GLYCEROKINASE FROM CANDIDA UTILIS

Glycerolkinase,E.coli

glycerolkinasefromcandidamycoderma

GLYCEROKINASE, FROM MICROORGANISM

ATP: glycerol 3-phosphotransferase, GK, Glycerokinase from Escherichia coli

ATP:glycerol 3-phosphotransferase, Glycerol Kinase

Glycerokinase from Streptomyces canus,ATP:glycerol 3-phosphotransferase, Glycerol Kinase

Glycerokinase from Escherichia coli,ATP:glycerol 3-phosphotransferase, Glycerol Kinase

Glycerokinase from Bacillus stearothermophilus,ATP:glycerol 3-phosphotransferase, Glycerol Kinase

Glycerokinase from Cellulomonas sp.,ATP:glycerol 3-phosphotransferase, Glycerol Kinase

Glycerokinase [ >=250 units/mg]

Glyceric kinase

Glycerin kinase

Glycerol phosphotransferase

GK/Glycerokinase

Glycerokinase, 150 units/Mg

Anti-MAP4K3, N-Terminal antibody produced in rabbit

MAPK/ERK kinase kinase kinase 3

MEK kinase kinase 3

Anti-GK, C-Terminal antibody produced in rabbit

GLK

HHF3

HK4

HKIV

Monoclonal Anti-GCK antibody produced in mouse

Glycerokinase, 150 units/mg protein

Glycerokinase from microorganisms

Kinase(phosphorylating),glycerol

GK

GLYCEROL KINASE

GLYCEROKINASE

EC 2.7.1.30

ATP: GLYCEROL 3-PHOSPHOTRANSFERASE

ANTI-GLYCEROL KINASE (GPK2)(C-TERMINAL) antibody produced in rabbit

testis specific 2

Anti-GK3, N-Terminal antibody produced in rabbit

Glycerol Kinase (GK)

glycerokinase from bacillus*stearothermophilus so

glycerokinase from cellulomonas species

glycerokinase from E coli

glycerokinase from escherichia coli

glycerokinase from streptomyces canus

glycerol kinase from E. coli

Glycerol Kinase from Escherichia coli

GLYCEROL KINASE FROM CANDIDA MYCODERMA, ~85 U/MG*

GLYCEROL, PCR REAGENT

GLYCEROKINASE FROM BACILLUS*STEAROTHERMO PHILUS SOLU

GLYCEROL KINASE FROM E. COLI LYOPH. &

GLYCEROKINASE FROM BACILLUS*STEAROTHERMO PHILUS

glycerokinase from cellulomonas sp.

Native Cellulomonas sp. Glycerokinase

Native Escherichia coli Glycerokinase

Native Streptomyces canus Glycerokinase

Native Microorganism Glycerol Kinase

Native Bacillus stearothermophilus Glycerokinase

GLYCEROKINASE USP/EP/BP

Glycerol Kinase (GK) ex. Microorganism, 30U/mg

Glycerol Kinase "Amano" 2

9030-66-4