Bleomycin hydrochloride
- Product Name
- Bleomycin hydrochloride
- CAS No.
- 67763-87-5
- Chemical Name
- Bleomycin hydrochloride
- Synonyms
- BleomycinHCl;bleomycinchlorhydrate;Suberic Acid Impurity 1;BLEOMYCIN HYDROCHLORIDE;BLEOMYCIN HYDROCHLORIDES;BleomycinA5/Pingyangmycin;BleoMycin A4A5 Hydrochloride;Bleomycin hydrochloride USP/EP/BP;Bleomycin, hydrochloride (9CI, ACI);Zeocin(bleomycin、phleomycin)Hydrochloride
- CBNumber
- CB8770570
- Molecular Formula
- C50H71N16O21S2R.x(HCl)
- Formula Weight
- 0
- MOL File
- Mol file
Bleomycin hydrochloride Property
- storage temp.
- 4°C, protect from light
- solubility
- DMSO: 125 mg/mL (86.15 mM)
- form
- Solid
- color
- White to light yellow
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H351Suspected of causing cancer
- Precautionary statements
-
P201Obtain special instructions before use.
P202Do not handle until all safety precautions have been read and understood.
P281Use personal protective equipment as required.
P308+P313IF exposed or concerned: Get medical advice/attention.
P405Store locked up.
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- CS-0131163
- Product name
- Bleomycin(hydrochloride)
- Purity
- 98.81%
- Packaging
- 10mg
- Price
- $168
- Updated
- 2021/12/16
- Product number
- CS-0131163
- Product name
- Bleomycin(hydrochloride)
- Purity
- 98.81%
- Packaging
- 50mg
- Price
- $672
- Updated
- 2021/12/16
Bleomycin hydrochloride Chemical Properties,Usage,Production
Originator
Bleomycin Hydrochloride,Nippon Kayaku, Co.,Japan
Uses
Bleomycin hydrochloride is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin hydrochloride is an antitumor antibiotic[1].
Manufacturing Process
To a medium having a composition of 6.4 % of millet jelly, 0.5 % of glucose,
3.5 % of soybean powder, 0.75 % of corn steep liquor, 0.3 % of sodium
chloride, 0.1 % of potassium secondary phosphate, 0.05 % of zinc sulfate,
0.01 % of copper sulfate, 0.2 % of sodium nitrate and 0.01 % of Toho No. 1
(trade name for a surface active agent composed of polyoxyethylene
manufactured by Toho Chemical Industry Co. Ltd., Japan) was added 3-aminopropyl-
dimethylsulfonium bromide hydrobromate in a proportion of 0.4 mg/ml
to adjust the pH of the medium to 6.5.
Each 100 ml of the thus treated medium was separately charged into a
Sakaguchi flask and was then sterilized. Subsequently, Streptomyces
verticillus (ATCC No. 15003) was inoculated in the medium and was cultured
at 27°C for 8 days with stirring at 130 r.p.m. Thereafter, the culture liquors
(4.5 L) were collected and filtered to obtain 3.0 L of a filtrate (potency 38.8
mg/ml, total potency 416.4 mg). This culture filtrate was passed through and
adsorbed on a column packed with 200 ml of Amberlite IRC-50 and was
washed with water and was eluted with 0.5 N hydrochloric acid. 1.0 L of the
eluate was neutralized, was passed through and adsorbed on a column packed
with 100 ml of active carbon, was washed and was then eluted by use of a
1:1 (by volume) mixture of acetone - 0.02 N aqueous hydrochloric acid
solution, and fractions active to Mycobacterium 607 were collected and
concentrated to dryness. The resulting residue was dissolved in 5 ml of an 80
% aqueous methanol solution and was charged into a column packed with 30
ml of neutral alumina, followed by elution with an 80 % aqueous methanol
solution. Subsequently, bleomycin-containing fractions were collected and
concentrated to dryness to obtain 195 mg of bleomycin hydrochloride
(potency 650.7 mcg/mg, total potency 172 mg). The yield from the culture
filtrate was 30.5 %.
Therapeutic Function
Antibiotic
in vivo
Bleomycin hydrochloride ((Bleomycin hydrochloride and Bleomycin sulfate are equivalent)) can be used to create animal models of pulmonary fibrosis. Intratracheal administration of Bleomycin sulfate (3.5-4.0 mg/kg) on day 0 elicits a treatment response, characterized by a decrease in body weight of the subjects on day 4, followed by recovery and continued growth until the end of the experiment. This treatment also significantly increases the levels of hydroxyproline in lung tissue and the mass of the right caudal lobe of the lung. In 8-week-old male BALB/c mice (weighing approximately 20-30 grams), intratracheal instillation of Bleomycin sulfate at a dose of 5.0 mg/kg/day induces pulmonary fibrosis and promotes the expression of α-SMA and type I collagen. Similarly, in 8-week-old male C57BL/6 mice (average weight around 24.5 grams), intratracheal injection of 2.5 mg/kg Bleomycin sulfate effectively induces pulmonary fibrosis. Bleomycin sulfate is rapidly absorbed after administration via intramuscular, subcutaneous, intraperitoneal, or intrapleural routes, reaching peak plasma concentrations within approximately 60 minutes. When given intravenously, less than 1% of the drug binds to plasma proteins, ensuring high bioavailability. The mean plasma clearance rate of Bleomycin sulfate is about 70 mL/min/m2, indicating high plasma elimination and urinary excretion rates[3][4][5][6].
Administration: 3-5 mg/kg ? intratracheal administration ? sprays on day one
Lung changes: Increased fibrotic consolidations, non-aerated lung area, and high-density lung area. Pulmonary function decreased.
Molecular changes: Increased indicators: TGF-β1, TNF-α, IL-6, and GM-CSF in bronchoalveolar lavage fluid.
| Animal Model: | Male Fischer 344 rats, 8-10 week old, weighing 150-250?g[3] |
| Dosage: | 3.5-4 mg/kg |
| Administration: | Intra-tracheal |
| Result: | Body weights decreased by day 4 then increased by Day 7 through the end of the study. |
References
[1] Hovhannisyan G, et al. Comparative analysis of individual chromosome involvement in micronuclei induced by bleomycin in human leukocytes. Mol Cytogenet. 2016 Jun 21;9:49. DOI:10.1186/s13039-016-0258-4
[2] Jaaskela-Saari HA, et al. Squamous cell cancer cell lines: sensitivity to bleomycin and suitability for animal xenograft studies. Acta Otolaryngol Suppl. 1997;529:241-4. DOI:10.3109/00016489709124133
[3] Corboz MR, et al. Therapeutic administration of inhaled INS1009, a LRX-15 prodrug formulation, inhibits bleomycin-induced pulmonary fibrosis in rats. Pulm Pharmacol Ther. 2018 Apr;49:95-103. DOI:10.1016/j.pupt.2018.01.012
[4] Kang Miao, et al. Scutellarein inhibits BLM-mediated pulmonary fibrosis by affecting fibroblast differentiation, proliferation, and apoptosis. Ther Adv Chronic Dis. 2020 Jul 30;11:2040622320940185. DOI:10.1177/2040622320940185
[5] Ling Peng, et al. Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. Cell Death Dis. 2020 Nov 13;11(11):978. DOI:10.1038/s41419-020-03178-2
Bleomycin hydrochloride Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Bleomycin hydrochloride manufacturers
- Product
- Bleomycin HCL 67763-87-5
- Price
- US $0.00/kg
- Min. Order
- 1kg
- Purity
- 0.99
- Supply Ability
- 1000kg
- Release date
- 2025-04-18
- Product
- Bleomycin hydrochloride 67763-87-5
- Price
- US $1.10/g
- Min. Order
- 1g
- Purity
- 99.9%
- Supply Ability
- 100 Tons Min
- Release date
- 2021-07-01
- Product
- Bleomycin hydrochloride 67763-87-5
- Price
- US $0.00-0.00/Kg
- Min. Order
- 1KG
- Purity
- 99.0%
- Supply Ability
- 800 ton
- Release date
- 2020-04-30