ChemicalBook > CAS DataBase List > EC 2.7.1.40

EC 2.7.1.40

Product Name
EC 2.7.1.40
CAS No.
9001-59-6
Chemical Name
EC 2.7.1.40
Synonyms
PK;EC 2.7.1.40;pyruvic kinase;PYRUVATE KINASE;PK （rabbit muscle);Pyruvate Kinase M2;PK,pyruvate kinase;EC 2.7.1.40 USP/EP/BP;pyruvate phosphoferase;PYRUVATE KINASE TYPE I
CBNumber
CB9396847
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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EC 2.7.1.40 Property

storage temp. 
-20°C
form 
powder
color 
white
Water Solubility 
Soluble in water ( 1mg/ml).
EPA Substance Registry System
Kinase (phosphorylating), pyruvate (9001-59-6)
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Safety

WGK Germany 
3
F 
10-21
TSCA 
Yes
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
P9136
Product name
Pyruvate Kinase from rabbit muscle
Purity
Type III, lyophilized powder, 350-600?units/mg protein
Packaging
1000units
Price
$79.4
Updated
2024/03/01
Sigma-Aldrich
Product number
P9136
Product name
Pyruvate Kinase from rabbit muscle
Purity
Type III, lyophilized powder, 350-600?units/mg protein
Packaging
5000units
Price
$199
Updated
2024/03/01
Sigma-Aldrich
Product number
SRP0414
Product name
PKLR Var2 active human
Purity
recombinant,expressedinE.coli,≥90%(SDS-PAGE)
Packaging
20μg
Price
$653
Updated
2024/03/01
Sigma-Aldrich
Product number
SRP0413
Product name
PKLR Var1 active human
Purity
recombinant,expressedinE.coli,≥43%(SDS-PAGE)
Packaging
20μg
Price
$653
Updated
2024/03/01
Sigma-Aldrich
Product number
P9136
Product name
Pyruvate Kinase from rabbit muscle
Purity
Type III, lyophilized powder, 350-600?units/mg protein
Packaging
25000units
Price
$602
Updated
2024/03/01
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EC 2.7.1.40 Chemical Properties,Usage,Production

Chemical Properties

White powder

Uses

Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency.

Uses

Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids.

Uses

Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.

General Description

Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells.

Biochem/physiol Actions

Molecular Weight: 237 kDa?and exists as a tetramer of four equal subunits of molecular weight 57 kDa.Isoelectric Point: 7.6Optimal pH: ～7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Purification Methods

These are purified by (NH4)2SO4 fractionation and gel filtration, ion-exchange and affinity chromatography. [Garcia-Olalla & Garrido-Pertierra Biochem J 241 573 1987.]

EC 2.7.1.40 Preparation Products And Raw materials

Raw materials

Ammonium sulfate

Preparation Products

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EC 2.7.1.40 Suppliers

China 126 Europe 2 France 1 Germany 4 India 3 Japan 3 Switzerland 1 United Kingdom 2 United States 12 Global 154
LIBIOS
Tel
--
Fax
--
Email
info@libios.fr
Country
France
ProdList
288
Advantage
58
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View Lastest Price from EC 2.7.1.40 manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Pyruvate Kinase 9001-59-6
Price
US $0.00-0.00/g
Min. Order
1g
Purity
98.0%
Supply Ability
1kg/month
Release date
2021-08-24
Shaanxi Dideu Medichem Co. Ltd
Product
EC 2.7.1.40 9001-59-6
Price
US $0.10/KG
Min. Order
1KG
Purity
99.0%
Supply Ability
1000 tons
Release date
2024-08-30
Hubei Xindesheng Material Technology Co., Ltd.
Product
Pyruvate Kinase 9001-59-6
Price
US $12.00-9.00/KU
Min. Order
10KU
Purity
≥ 25.0U/mg
Supply Ability
10000ku
Release date
2020-11-04

9001-59-6, EC 2.7.1.40Related Search:

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  • ATP:PYRUVATE 2-O-PHOSPHOTRANSFERASE
  • ATP: PYRUVATE O2-PHOSPHOTRANSFERASE
  • EC 2.7.1.40
  • PK （rabbit muscle)
  • pyruvate phosphoferase
  • PYRUVATE KINASE FROM RABBITMUSCLE CA. 350 U/MG PROTEIN SUSP.
  • Pyruvate Kinase from Bacillus stearothermophilus,ATP:pyruvate 2-O-phosphotransferase, PK
  • Pyruvate Kinase from rabbit muscle,ATP:pyruvate 2-O-phosphotransferase, PK
  • PYRUVATE KINASE, PRESTAINED FROM*CHICKEN MUSCLE
  • PK(R.M.), ATP: pyruvate 2-o-phosphotransferase
  • Pyruvate kinase (from Rabbit muscle), ammonium sulfate suspension
  • Pyruvat kinase from rabbit, lyophil.
  • PHOSPHOENOL PYRUVATE KINASE
  • PK
  • PYRUVATE KINASE
  • PYRUVATE KINASE, RABBIT MUSCLE
  • PYRUVATE KINASE TYPE I
  • PYRUVATE KINASE TYPE II
  • PYRUVATE KINASE TYPE III
  • PYRUVATE KINASE TYPE VIII
  • PYRUVATE O2-PHOSPHO TRANSFERASE
  • Pyruvate Kinase, 250 units/Mg
  • Pyruvate Kinase froM MicroorganisM
  • Pyruvate Kinase from rabbit liver
  • Pyruvate Kinase M2
  • pyruvate kinase from rabbit muscle
  • pyruvate kinase from rabbit muscle cryst susp. ~350 U/mg
  • Pyruvate Kinase preparation from rabbit muscle
  • pyruvate kinase type I crude*from rabbit muscle
  • pyruvate kinase type iii from rabbit*muscle
  • pyruvate kinase type viii from*bacillus stearothe
  • pyruvate kinase type X from chicken*muscle
  • pyruvate kinase type xii from rabbit*liver
  • Kinase (phosphorylating), pyruvate
  • PYRUVATE KINASE TYPE X FROM CHICKEN &
  • PYRUVATE KINASE TYPE VII FROM*RABBIT MUS CLE
  • PYRUVATE KINASE PRESTAINED FROM CHICKEN
  • PYRUVATE KINASE FROM RABBIT MUSCLE, 100- 250 U/MG
  • PYRUVATE KINASE TYPE VIII FROM*BACILLUS STEAROTHERM
  • PYRUVATE KINASE TYPE II FROM RABBIT*MUSC LE
  • PYRUVATE KINASE (EC 2.7.1.40) FROM RABBIT MUSCLE LYOPHILIZED POWDER ESSENTIALLY SALT-FREE
  • PYRUVATE KINASE FROM PORCINE HEART (E.C. 2.7.1.40)
  • PYRUVATE KINASE (EC 2.7.1.40) FROM RABBIT MUSCLE HIGHLY PURIFIED SUSPENSION
  • PYRUVATE KINASE FROM BACILLUS STEAROTHERMOPHILUS
  • PyruvateKinaseExRabbitMuscle
  • Pyruvate kinase from rabbit muscle ca. 350 U/mg protein
  • pyruvic kinase
  • ATP:pyruvate 2-O-phosphotransferase, PK
  • Pyruvate Kinase preparation from canine muscle
  • phosphopyruvate transphos phorylase
  • Pyruvate Kinase, 50 units/mg
  • Pyruvate Kinase M2 from Human, Recombinant
  • Pyruvate kinase M2 Active from Mouse, Recombinant
  • Native Pyruvate Kinase from Thermophillic bacteria
  • Native Bacillus stearothermophilus Pyruvate Kinase
  • EC 2.7.1.40 USP/EP/BP
  • Pyruvate Kinase ex. Rabbit Muscle, 300U/mg
  • PK,pyruvate kinase