PULLULANASE Chemical Properties

Density 

1.25 g/mL at 25 °C(lit.)

vapor pressure 

0Pa at 25℃

storage temp. 

2-8°C

form 

lyophilized powder

color 

Light yellow to brown

Water Solubility 

100g/L at 25℃

LogP

-1.3 at 20℃

CAS DataBase Reference

9075-68-7

EPA Substance Registry System

Pullulanase (9075-68-7)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

PULLULANASE Usage And Synthesis

Description

As an an amylolytic exoenzyme, Pullulanase is one of the most essential endo-type enzyme that degrades pullulan. It is found in plants and microorganisms, in which it is synthesized as an extracellular, cell surface-anchored lipoprotein by the Gram-negative bacterium such as Bacillus brevis, genus Klebsiella. Type I pullulanases specifically cleaves α-1,6-glucosidic linkages in polysaccharides, such as starch, amylopectin, glycogen, dextrin, pullulan and related oligosaccharides, whilst type II pullulanases are effective to attack α-1,6-glucosidic linkages. This enzyme is applied on a large scale in glucose and maltose syrup industries, where it is combined with the activities of other enzymes, such as β-amylase and isoamylase to manufacture maltose and oligosaccharides. Besides, it commercially serves as an industrial enzyme preparation in various reactions, such as the production of branched cyclodextrins, where pullulanase can catalyze the condensation reaction. Pullulanase is also applied as a processing aid in grain processing biotechnology for the production of ethanol and sweeteners.

References

https://en.wikipedia.org/wiki/Pullulanase
http://www.sigmaaldrich.com/catalog/product/sigma/e2412?lang=en&region=US
https://www.hindawi.com/journals/er/2012/921362/
http://www.amano-enzyme.co.jp/eng/enzyme/15.html
http://www.ssjournals.com/index.php/ijbr/article/view/610

Uses

One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25°C.

Uses

Pullulanase is a glucanase that degrades pullulan. It is produced by the Gram-negative bacterium Klebsiella and is used for starch-debranching as well as to study polysaccharide utilization.

Biochem/physiol Actions

Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.

PULLULANASE(9075-68-7)Related Product Information

• AMYLOPECTIN
• TERT-BUTYL ISOCYANIDE
• 2,4-PENTANEDIONE, SILVER DERIVATIVE
• DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)
• SALCOMINE
• Ferric acetylacetonate
• METHYL ISOCYANOACETATE
• Cupric acetylacetonate
• Aluminum acetylacetonate
• Benzyl isocyanide
• PHENYLSELENOL
• Tris(2,4-pentanedionato)chroMiuM(III)
• Tosylmethyl isocyanide
• Ethyl isocyanoacetate
• COBALT(II) ACETYLACETONATE
• MALTOTRIOSE