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Thrombin

Basic information Safety Supplier Related

Thrombin Basic information

Product Name:
Thrombin
Synonyms:
  • Blood coagulation factor IIa
  • C00752
  • Thrombin from human plasma,Factor IIa
  • Thrombin from murine plasma,Factor IIa
  • Thrombin from bovine plasma,Factor IIa
  • THROMBIN
  • THROMBIN, BOVINE
  • ThroMbin froM bovine plasMa 1000 units/Mg protein lyophil.
CAS:
9002-04-4
MF:
NULL
MW:
0
EINECS:
232-648-7
Product Categories:
  • proteins
Mol File:
Mol File
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Thrombin Chemical Properties

Melting point:
47.7 °C
storage temp. 
-20°C
solubility 
H2O: 1 mL/vial clear to slightly hazy, colorless solution
form 
powder
color 
white
Merck 
13,9461
EPA Substance Registry System
Thrombin (9002-04-4)
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Safety Information

Hazard Codes 
Xn,B,Xi
Risk Statements 
36/37/38-42
Safety Statements 
22-24/25-36/37-26-24
WGK Germany 
2
RTECS 
XO8950000
TSCA 
Yes
HS Code 
30021099

MSDS

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Thrombin Usage And Synthesis

Description

In order to stop local bleeding, thrombin, a natural thrombin drug that catalyzes the transformation of fibrinogen to fibrin, is frequently used. Thrombin is synthesized from cow plasma, and it is used to stop bleeding from open vessels when it is not possible to use other methods. It is used to stop light bleeding.

Uses

Thrombin is a trypsin-like serine protease involved in a multitude of processes in the human body. Thrombin generation is the result of limited proteolysis of the vitamin K-dependent zymogen prothrombin. Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to fibrin which forms the fibrin gel of a haemostatic plug or a pathologic thrombus

Uses

Thrombostat (Parke-Davis).

Uses

Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.

General Description

Thrombin is a sterile protein substanceprepared from prothrombin of bovine origin. It is used as atopical hemostatic because it can clot blood, plasma, or a solutionof fibrinogen without addition of other substances.Thrombin also may initiate clotting when combined withgelatin sponge or fibrin foam.
For external use it is applied topically to the wound, as asolution containing 100 to 2,000 National Institutes ofHealth (NIH) units/mL in sodium chloride irrigation or sterilewater for injection or as a dry powder.

Biochem/physiol Actions

The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles of the brain may contribute to the aggregation of tau protein and pathophysiology of Alzheimer disease.

Purification Methods

Thrombin is purified by chromatography on a DEAE-cellulose column, while eluting with 0.1M NaCl, pH 7.0, followed by chromatography on Sephadex G-200. The final preparation is free from plasminogen and plasmin. [Yin & Wessler J Biol Chem 243 112 1968.] Thrombin from bovine blood is also purified by chromatography using p-chlorobenzylamino--aminocaproyl agarose, and gel filtration through Sephadex G-25. [Thompson & Davie Biochim Biophys Acta 250 210 1971.] Thrombin from various species was purified initially by precipitation of impurities with rivanol. [Miller Nature 184 450 1959.] Tissue inhibitor of metalloproteins (TIMP, from human blood plasma), Mr ~30,000. These are purified by an [anti-human amniotic fluid-TIMP]-Sepharose immuno-affinity column and eluted with 50mM glycine/HCl pH 3.0 buffer that is 0.5M in NaCl, and followed by gel filtration [Cawston et al. Biochem J 238 677 1986].

Thrombin Preparation Products And Raw materials

Raw materials

ThrombinSupplier

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