3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING] Chemical Properties

storage temp. 

-20°C

form 

partially purified powder

biological source

spinach

Specific Activity

0.01-0.1unit/mg solid

Safety Information

WGK Germany 

3

Storage Class

11 - Combustible Solids

Hazard Classifications

Resp. Sens. 1

MSDS

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3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING] Usage And Synthesis

Uses

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:

• as a test protein in pepsin digestion studies
• as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
• in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid

General Description

Exists as a 557kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56kDa and eight light chains of molecular weight 14kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
K_M for CO₂: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Biochem/physiol Actions

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.

3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING](9027-23-0)Related Product Information

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