DABCYL-TYR-VAL-ALA-ASP-ALA-PRO-VAL-EDANS Chemical Properties

Density 

1.38±0.1 g/cm3(Predicted)

storage temp. 

-15°C

solubility 

5% NH3 (Aqueous): 1 mg/ml

form 

A lyophilized powder

pka

-0.26±0.40(Predicted)

DABCYL-TYR-VAL-ALA-ASP-ALA-PRO-VAL-EDANS Usage And Synthesis

Description

Dabcyl-YVADAPV-EDANS is a fluorogenic substrate for caspase-1. Upon enzymatic cleavage by caspase-1. EDANS is released upon enzymatic cleavage by caspase-1 at the Ala-Ser site, of which the fluorescence can be used to quantify caspase-1 activity.

Uses

Dabcyl-YVADAPV-EDANS is a fluorogenic interleukin-1β converting enzyme (ICE) substrate for measuring ICE-like protease activity. Dabcyl-YVADAPV-EDANS detects fluorescence at 360 nm excitation and 480 nm emission wavelengths. ICE-like protease is a critical mediator of K+ deprivation-induced apoptosis of cerebellar granule neurons[1].

References

[1] Schulz JB, et al. Potassium deprivation-induced apoptosis of cerebellar granule neurons: a sequential requirement for new mRNA and protein synthesis, ICE-like protease activity, and reactive oxygen species. J Neurosci. 1996 Aug 1;16(15):4696-706. DOI:10.1523/JNEUROSCI.16-15-04696.1996

Sequene

Dabcyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-edans

DABCYL-TYR-VAL-ALA-ASP-ALA-PRO-VAL-EDANS(161877-70-9)Related Product Information

• CALCIUM-LIKE PEPTIDE
• Dentonin
• H-TYR-CYS-TRP-SER-GLN-TYR-LEU-CYS-TYR-OH
• COG-1410
• HNGF6A
• -Defensin-2 (human) (trifluoroacetate salt)
• L-Glutamine, L-leucyl-L-lysyl-L-lysyl-L-threonyl-L-α-glutamyl-L-threonyl-
• L-Cysteine, L-cysteinylglycyl-L-asparaginyl-L-lysyl-L-arginyl-L-threonyl-L-arginylglycyl-, cyclic (1→9)-disulfide
• C-PEPTIDE 2 (RAT)
• 4-DIMETHYLAMINO-4'-METHYLAZOBENZENE
• 1,5-EDANS
• BZ-PHE-NH2
• BZ-TYR-VAL-NH2
• AC-YVAD-CHO
• 4-amino-N-(2-phenylethyl)benzamide
• DABCYL-TYR-VAL-ALA-ASP-ALA-PRO-VAL-EDANS
• BZ-TYR-NH2
• Caspase 1, beta (Interleukin-1 Beta Converting Enzyme, ICE) Substrate II [DABCYL-Tyr-Val-Ala-Asp-Ala-Pro-Val-EDANS]