Laccase Chemical Properties

Density 

1.37[at 20℃]

vapor pressure 

0.004Pa at 25℃

storage temp. 

2-8°C

form 

powder

color 

slightly beige

Water Solubility 

100g/L at 25℃

LogP

-1.3 at 20℃

EPA Substance Registry System

Laccase (80498-15-3)

Safety Information

Hazard Codes 

Xn

Risk Statements 

42

Safety Statements 

23-45-24/25

WGK Germany 

1

HS Code 

35079090

MSDS

• Language:English Provider:SigmaAldrich

Laccase Usage And Synthesis

Description

Laccase is a kind of copper-containing oxidase existing in many kinds of plants, fungi and microbes. It mainly takes effect on phenol-like compounds including phenols, polyphenols and anilines, performing one-electron oxidation. It is capable of catalyzing the ring cleavage reaction of aromatic compounds. In industry, laccase can be paired with an electron mediator to facilitate electron transfer to a solid electrode wire. It can also be applied to textile dyeing/textile finishing, wine cork making, teeth whitening with many other industrial, environmental, diagnostic, and synthetic uses. In food industry, laccase can be used to remove the polyphenols as well as oxygen that remain in the beer to increase the storage life of beer. Laccase can also be used in fruit juices to delay the oxidation of polyphenols and stabilize the juice.

References

https://en.wikipedia.org/wiki/Laccase
http://172.16.24.174/digital.csic.es/bitstream/10261/9595/1/postprint_laccase_patent_review.pdf

Uses

Laccase from Trametes versicolor has been used:

• to assess the use of four laccase-producing strains in waste water treatment
• in laccase assay
• in screening the lignols

Uses

Laccase is polyphenol oxidase found in many plants, fungi and microorganisms. Laccases may be useful in enzymatic biofuel systems, teeth whitening, textile dyeing, and in other applications that require the removal of oxygen .

Uses

Laccases are multicopper-oxidases (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) that are able to oxidize phenolic substrates (e.g. 2,6-dimethoxy-phenol), aromatic amines (e.g. 1-hydroxybenzotriazole), or polycyclic aromatic hydrocarbons (e.g. anthracene) . The oxidation of the substrate occurs via a oneelectron reduction and is accompanied by a reduction of molecular oxygen to water.
Most laccases are of fungal origin, but they also occur in bacteria, insects, and plants. Due to the broad substrate range of laccases, their possible industrial usage is widespread. Nevertheless, only few applications have been commercialized up to now, mostly in the textile industry. In the food and feed sector, laccases have been evaluated for different applications, such as the stabilization of beverages, the reduction of off-flavors, the improvement of wheat dough, and the usage of laccases as biosensors in the food processing industry. Off-flavors in wine may occur due to microbial conversion of phenolic compounds present in the wine itself or in the cork stoppers. In a commercial product from Novozymes called Suberase, laccase is used for polymerization of phenolic compounds in the cork, which act as precursors for malodors, such as pentachlorophenol or 2,4,6-trichloroanisol.
In apple juice, addition of laccase reduced the amount of the phenolic offflavors 2,6-dibromophenol, guaiacol, and a-terpineol. Several studies used laccases to increase the stability of apple juices by polymerization of phenols and their subsequent removal by ultrafiltration methods. A positive side effect is the decrease of molecular oxygen in the juice due to its consumption by laccases. Nevertheless, desired phenols might also be oxidized; thus, the sensory attributes and nutritional value might be altered.
The polymerization reactions catalyzed by laccases can also be used to improve the shade of food, such as the coloration of tea-based products. In the bakery industry, laccase might be used together with proteases or xylanases to improve the dough quality. It was proposed that laccases oxidize ferulic acid attached to the arabinoxylan present in cereal flour. The obtained phenolic radicals can undergo a nonenzymatic reaction, resulting in cross-linked feruloylated arabinoxylans. In oat flour-based bread, the usage of laccase increased the loaf-specific volume and decreased the crumb hardness. Contradictory, in another study, laccase alone decreased the specific volume and increased the crumb hardness. A combined usage of laccase with xylanase improved again the oat flour bread properties.

General Description

Laccase EC 1.10.3.2, a glycoprotein, is an extracellular multicopper enzyme and is considered as a metal. Laccase is widely distributed in fungi and also found among the higher plants, bacteria and insects.

Flammability and Explosibility

Not classified

Biochem/physiol Actions

Laccase oxidizes aromatic and nonaromatic compounds. Various compounds are used for detecting laccase production. These include guaiacol, syringaldazine and polymeric dyes like remazol brilliant blue-R. Laccase is involved in lignin degradation and thereby has industrial as well as food applications. Laccase is commonly used for delignification, dye bleaching, paper processing, waste detoxification, textile dye transformation, plant fiber modification and ethanol production.

Biotechnological Applications

Lee et al. isolated and identified an extracellular laccase-producing strain of Y. lipolytica from soil. Extracellular laccase (YlLac) was purified by anionexchange and gel filtration chromatography. YlLac is a monomeric glycoprotein with 14 % carbohydrate content and a molecular weight of 67 kDa. It showed a higher catalytic efficiency toward 2,20-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (kcat/Km = 19.3 s-1 lM-1) and 2,6-dimethoxyphenol (kcat/Km = 13 s-1 lM-1) than any other reported laccases. This enzyme was able to oxidize phenolic compounds of pretreated rice straw. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries is a promising approach for improving the efficiency of biorefineries (Lee et al. 2012).

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