Iodonitrotetrazolium chloride Chemical Properties

Melting point:

240 °C (dec.)(lit.)

storage temp. 

Store at 2-8

solubility 

methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow

form 

Powder, Crystals and/or Chunks

color 

White to off-white

Water Solubility 

soluble

λmax

248nm

Sensitive 

Light Sensitive

BRN 

4093224

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer

InChIKey

JORABGDXCIBAFL-UHFFFAOYSA-M

CAS DataBase Reference

146-68-9(CAS DataBase Reference)

EPA Substance Registry System

2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9)

Safety Information

Hazard Codes 

Xn,F,Xi

Risk Statements 

20/21/22-68/20/21/22-36/37/38-11

Safety Statements 

36/37-36/37/39-26-22-16

RIDADR 

2811

WGK Germany 

3

F 

3-8-10

TSCA 

Yes

HazardClass 

6.1

PackingGroup 

III

HS Code 

29339900

MSDS

• Language:English Provider:2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
• Language:English Provider:SigmaAldrich

Iodonitrotetrazolium chloride Usage And Synthesis

Chemical Properties

slightly yellow powder

Uses

Electron acceptor for the colorimetric assay of various dehydrogenases.

Definition

ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.

Purification Methods

Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]

Iodonitrotetrazolium chloride(146-68-9)Related Product Information

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