4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide

Product Name: 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide
CAS No.: 312756-74-4
Chemical Name: 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide
Synonyms: RS-1;CS-2312;RS1;RS 1;RAD51-stimulatory compound 1;RAD51-Stimulatory Compound-1, RS-1;RS-1 - RAD51-Stimulatory Compound-1;RAD51,RS1,RS-1,inhibit,Inhibitor,RS 1,CRISPR/Cas9;3-benzylsulfamoyl-4-bromo-N-(4-bromophenyl)benzamide;3-(N-Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide;RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem
CBNumber: CB02695334
Molecular Formula: C20H16Br2N2O3S
Formula Weight: 524.23
MOL File: 312756-74-4.mol

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4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Property

Density: 1.680±0.06 g/cm3(Predicted)
storage temp.: room temp
solubility: DMSO: ≥10mg/mL
form: powder
pka: 10.16±0.30(Predicted)
color: off-white to light tan
Stability:: Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months.
InChIKey: VBOWCZDVEOFGRY-UHFFFAOYSA-N
CAS DataBase Reference: 312756-74-4

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Safety

Hazard Codes: Xi
Risk Statements: 36
Safety Statements: 26
WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word: Warning
Hazard statements: H319Causes serious eye irritation
Precautionary statements: P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: R9782
Product name: RS-1
Purity: ≥98% (HPLC)
Packaging: 5mg
Price: $44.4
Updated: 2024/03/01

Sigma-Aldrich

Product number: 553510
Product name: RAD51-Stimulatory Compound-1, RS-1
Packaging: 10mg
Price: $155
Updated: 2024/03/01

Cayman Chemical

Product number: 21037
Product name: RS-1
Purity: ≥95%
Packaging: 5mg
Price: $32
Updated: 2024/03/01

Cayman Chemical

Product number: 21037
Product name: RS-1
Purity: ≥95%
Packaging: 10mg
Price: $57
Updated: 2024/03/01

Sigma-Aldrich

Product number: R9782
Product name: RS-1
Purity: ≥98% (HPLC)
Packaging: 25mg
Price: $449
Updated: 2024/03/01

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4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Chemical Properties,Usage,Production

Description

RS-1 is an activator of DNA repair protein RAD51 (K_d = 48-107 for human RAD51 with different cofactors present). It stimulates homologous strand assimilation activity at least 5- to 11-old, enhancing homologous recombination activity of hRAD51. RS-1 is a potent enhancer of CRISPR-mediated genome editing, increasing homology directed repair 3- to 6-fold. It is used to increase CRISPR-mediated knock-in efficiencies in vitro and in vivo.

Uses

4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]benzamide is a stimulant of the human homologous recombination protein RAD51.

Uses

RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.

General Description

A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis. Although RS-1 enhances hRAD51 filament formation on ssDNA with or without the cofactor NTP, active filaments and recombinase activity are only induced in the presence of ATP or AMP-PNP, but not with ADP or no cofactors. Shown to promote resistance of primary human neonatal dermal fibroblasts to Cisplatin- (Cat. No. 232120) induced death in a dose-dependent manner. RS-1 is inactive toward related DNA strand exchange proteins scRAD5 and scDMC1 of yeast origin or E. coli RedA.This HDR (homology-directed repair) enhancer, is shown to significantly increase both Cas9 & TALEN-mediated knock-in efficiencies.

Biological Activity

RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer that enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.

Biochem/physiol Actions

RS-1 is a sulfonamido-benzamide compound.

in vitro

RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity[1]. RS-1 enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.

storage

Room temperature

References

1) Jayathilaka et al. (2008), A chemical compound that stimulates the human homologous recombination protein RAD51; Proc. Natl. Acad. Sci. USA 105 15848 2) Mason et al. (2014), The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors; Cancer Res. 74 3546 3) Pinder et al. (2015), Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing; Nucleic Acids Res. 43 9379 4) Song et al. (2016), RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency; Nat. Commun. 7 10548

4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Preparation Products And Raw materials

Raw materials

Preparation Products

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4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Suppliers

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View Lastest Price from 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide manufacturers

Career Henan Chemical Co

Product: 3-(Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide 312756-74-4
Price: US $1.00/KG
Min. Order: 1KG
Purity: Min98% HPLC
Supply Ability: g/kg/ton
Release date: 2020-01-01

312756-74-4, 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMideRelated Search:

xav-939 ME0328 GPI-16552 (GPI16552) PJ-34 FORMANILIDE 4-Aminodiphenylamine AZD6738 SGC707 MS023 2-(2-Chlorophenyl)-4-(3-(diMethylaMino)phenyl)-5-Methyl-1H-pyrazolo[4,3-c]pyridine-3,6(2H,5H)-dione 3,4-dimethoxy-N-[4-(3-nitrophenyl)-1,3-thiazol-2-yl]benzenesulfonamide Enasidenib Epacadostat (INCB024360) indoleaMine-2,3-dioxygenase inhibitor INCB024360 LLY-507 DBeQ

4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide

RAD51-Stimulatory Compound-1, RS-1

RAD51-stimulatory compound 1

RS-1

CS-2312

RS1;RS 1

RS-1 - RAD51-Stimulatory Compound-1

3-benzylsulfamoyl-4-bromo-N-(4-bromophenyl)benzamide

RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem

Benzamide, 4-bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-

3-(N-Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide

RAD51,RS1,RS-1,inhibit,Inhibitor,RS 1,CRISPR/Cas9

312756-74-4

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