ChemicalBook > CAS DataBase List > Neutral Red

Neutral Red

Product Name
Neutral Red
CAS No.
553-24-2
Chemical Name
Neutral Red
Synonyms
Neutral Red;N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride;Kernechrot;C.I. 50040;neutralredw;Basic Red 5;c.i.basicred5;michromeno.226;cervenneutralni;cervenzasadita5
CBNumber
CB7853810
Molecular Formula
C15H17ClN4
Formula Weight
288.78
MOL File
553-24-2.mol
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Neutral Red Property

Melting point:
290 °C (dec.) (lit.)
Boiling point:
441.31°C (rough estimate)
Density 
1.1590 (rough estimate)
bulk density
350-500kg/m3
refractive index 
1.6110 (estimate)
storage temp. 
Store at +5°C to +30°C.
solubility 
H2O: soluble10mg/mL
Colour Index 
50040
pka
6.7, 7.4(at 25℃)
form 
Solid
color 
Very dark green
PH
3.1 (10g/l, H2O, 20℃)
PH Range
6.8(red)-8(yellow)
Odor
Odorless
Appearance
Solid Powder
Water Solubility 
50 g/L
λmax
540nm, 533nm, 544nm, 529nm, 454nm
Merck 
14,6488
BRN 
3918740
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases
Major Application
Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases
CAS DataBase Reference
553-24-2(CAS DataBase Reference)
EPA Substance Registry System
Neutral Red (553-24-2)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
68-36/37/38
Safety Statements 
36/37-37/39-36-26-24/25
WGK Germany 
3
RTECS 
SG1400000
TSCA 
Yes
HS Code 
32041300
Toxicity
LD50 intraperitoneal in mouse: 432mg/kg
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
72210
Product name
Neutral Red
Purity
for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)
Packaging
5g
Price
$33.9
Updated
2025/07/31
Sigma-Aldrich
Product number
1.01369
Product name
Neutral red (C.I. 50040)
Purity
indicator and for microbiology
Packaging
25g
Price
$213
Updated
2025/07/31
Sigma-Aldrich
Product number
1.01369
Product name
Neutral red (C.I. 50040)
Purity
indicator and for microbiology
Packaging
100g
Price
$743
Updated
2025/07/31
Sigma-Aldrich
Product number
1.01376
Product name
Neutral red (C.I. 50040)
Purity
for microscopy Certistain?
Packaging
25g
Price
$149
Updated
2023/01/07
TCI Chemical
Product number
N0315
Product name
Basic Red 5
Packaging
25g
Price
$56
Updated
2025/07/31
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Neutral Red Chemical Properties,Usage,Production

Description

Neutral red is useful as a biological stain and as an indicator for preparing neutral red paper.

Chemical Properties

dark green powder

Uses

Can be used as a vital stain, to stain living cells.

Uses

Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.

Definition

ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).

Application

Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.

Preparation

(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.

General Description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Biotechnological Applications

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

storage

+4°C

Staining Procedures

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

Properties and Applications

red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation.

Purification Methods

In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]

Neutral Red Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Neutral Red manufacturers

RongNa Biotechnology Co.,Ltd
Product
Neutral Red 553-24-2
Price
US $10.00/ASSAYS
Min. Order
1ASSAYS
Purity
99%
Supply Ability
1 ton
Release date
2025-08-27
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Basic Red 5 553-24-2
Price
US $0.00/KG
Min. Order
1KG
Purity
98%min
Supply Ability
30tons/month
Release date
2023-01-10
Jiangsu Boquan Biotechnology Co., Ltd.
Product
Basic Red 5 553-24-2
Price
US $190.00-170.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
1tons
Release date
2023-02-21

553-24-2, Neutral RedRelated Search:

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