Neutral Red

Product Name: Neutral Red
CAS No.: 553-24-2
Chemical Name: Neutral Red
Synonyms: Neutral Red;N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride;Kernechrot;C.I. 50040;neutralredw;Basic Red 5;c.i.basicred5;michromeno.226;cervenneutralni;cervenzasadita5
CBNumber: CB7853810
Molecular Formula: C15H17ClN4
Formula Weight: 288.78
MOL File: 553-24-2.mol

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Neutral Red Property

Melting point:: 290 °C (dec.) (lit.)
Boiling point:: 441.31°C (rough estimate)
Density: 1.1590 (rough estimate)
refractive index: 1.6110 (estimate)
storage temp.: Store at +5°C to +30°C.
solubility: H2O: soluble10mg/mL
Colour Index: 50040
pka: 6.7, 7.4(at 25℃)
form: Solid
color: Very dark green
PH: 3.1 (10g/l, H2O, 20℃)
PH Range: 6.8(red)-8(yellow)
Odor: Odorless
Water Solubility: 50 g/L
λmax: 540nm, 533nm, 544nm, 529nm, 454nm
Merck: 14,6488
BRN: 3918740
Stability:: Stable. Incompatible with strong oxidizing agents.
Biological Applications: Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases
Major Application: Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases
CAS DataBase Reference: 553-24-2(CAS DataBase Reference)
EPA Substance Registry System: Neutral Red (553-24-2)

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Safety

Hazard Codes: Xn,Xi
Risk Statements: 68-36/37/38
Safety Statements: 36/37-37/39-36-26-24/25
WGK Germany: 3
RTECS: SG1400000
TSCA: Yes
HS Code: 32041300
Toxicity: LD50 intraperitoneal in mouse: 432mg/kg

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 72210
Product name: Neutral Red
Purity: for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)
Packaging: 5g
Price: $32.7
Updated: 2024/03/01

Sigma-Aldrich

Product number: 1.01369
Product name: Neutral red (C.I. 50040)
Purity: indicator and for microbiology
Packaging: 25g
Price: $202
Updated: 2024/03/01

Sigma-Aldrich

Product number: 1.01369
Product name: Neutral red (C.I. 50040)
Purity: indicator and for microbiology
Packaging: 100g
Price: $709
Updated: 2024/03/01

Sigma-Aldrich

Product number: 1.01376
Product name: Neutral red (C.I. 50040)
Purity: for microscopy Certistain?
Packaging: 25g
Price: $149
Updated: 2023/01/07

TCI Chemical

Product number: N0315
Product name: Basic Red 5
Packaging: 25g
Price: $56
Updated: 2024/03/01

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Neutral Red Chemical Properties,Usage,Production

Chemical Properties

dark green powder

Uses

Can be used as a vital stain, to stain living cells.

Uses

Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.

Definition

ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).

Application

Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.

Preparation

(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.

General Description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Biotechnological Applications

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

storage

+4°C

Staining Procedures

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

Properties and Applications

red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation.

Purification Methods

In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]

Neutral Red Preparation Products And Raw materials

Raw materials

Preparation Products

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Neutral Red Suppliers

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View Lastest Price from Neutral Red manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Basic Red 5 553-24-2
Price: US $0.00/KG
Min. Order: 1KG
Purity: 98%min
Supply Ability: 30tons/month
Release date: 2023-01-10

Jiangsu Boquan Biotechnology Co., Ltd.

Product: Basic Red 5 553-24-2
Price: US $190.00-170.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 1tons
Release date: 2023-02-21

Zhuozhou Wenxi import and Export Co., Ltd

Product: Basic Red 5 553-24-2
Price: US $15.00-10.00/KG
Min. Order: 1KG
Purity: 99%+ HPLC
Supply Ability: Monthly supply of 1 ton
Release date: 2021-07-11

553-24-2, Neutral RedRelated Search:

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neutral red solution (0.33%)

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N8,N8,3-trimethyl-2,8-phenazinediamine monohydrochloride

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NEUTRAL RED PURIFIED