Neutral Red Property

Melting point:

290 °C (dec.) (lit.)

Boiling point:

441.31°C (rough estimate)

Density 

1.1590 (rough estimate)

refractive index 

1.6110 (estimate)

storage temp. 

Store at +5°C to +30°C.

solubility 

H2O: soluble10mg/mL

Colour Index 

50040

pka

6.7, 7.4(at 25℃)

form 

Solid

color 

Very dark green

PH

3.1 (10g/l, H2O, 20℃)

PH Range

6.8(red)-8(yellow)

Odor

Odorless

Water Solubility 

50 g/L

λmax

540nm, 533nm, 544nm, 529nm, 454nm

Merck 

14,6488

BRN 

3918740

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases

Major Application

Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases

CAS DataBase Reference

553-24-2(CAS DataBase Reference)

EPA Substance Registry System

Neutral Red (553-24-2)

Safety

Hazard Codes 

Xn,Xi

Risk Statements 

68-36/37/38

Safety Statements 

36/37-37/39-36-26-24/25

WGK Germany 

3

RTECS 

SG1400000

TSCA 

Yes

HS Code 

32041300

Toxicity

LD50 intraperitoneal in mouse: 432mg/kg

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

Neutral Red Chemical Properties,Usage,Production

Chemical Properties

dark green powder

Uses

Can be used as a vital stain, to stain living cells.

Uses

Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.

Definition

ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).

Application

Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.

Preparation

(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.

General Description

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Biochem/physiol Actions

Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Biotechnological Applications

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

storage

+4°C

Staining Procedures

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

Properties and Applications

red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation.

Purification Methods

In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]