Elastase
- Product Name
- Elastase
- CAS No.
- 39445-21-1
- Chemical Name
- Elastase
- Synonyms
- Elastase;Elastasee;EC 3.4.21.37;EC 3.4.21.36;Elastase, Purified;LYSOSOMAL ELASTASE;ELASTASE, LEUKOCYTE;Elastase USP/EP/BP;PANCREATOPEPTIDASE E;Elastase (pig pancreas)
- CBNumber
- CB8394260
- Molecular Formula
- NULL
- Formula Weight
- 0
- MOL File
- Mol file
Elastase Property
- RTECS
- TS5052000
- storage temp.
- -20°C
- solubility
- H2O: soluble
- form
- lyophilized powder
- color
- white
- Water Solubility
- Soluble in water.
Safety
- Hazard Codes
- B,Xn
- Risk Statements
- 36/37/38-42
- Safety Statements
- 23-24-26-36/37-22
- WGK Germany
- 3
- F
- 3-10
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H334May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- 324682
- Product name
- Elastase, Porcine Pancreas, High Purity, Crystallized
- Packaging
- 250u
- Price
- $173
- Updated
- 2024/03/01
- Product number
- 324682
- Product name
- Elastase, Porcine Pancreas, High Purity, Crystallized
- Packaging
- 1000u
- Price
- $520
- Updated
- 2024/03/01
- Product number
- J61874
- Product name
- Elastase, porcine pancreas
- Packaging
- 1g
- Price
- $1751
- Updated
- 2023/06/20
- Product number
- J61753
- Product name
- Elastase, porcine pancreas aqueous suspension
- Packaging
- 100mg
- Price
- $227
- Updated
- 2023/06/20
- Product number
- E7885
- Product name
- Elastase from porcine pancreas
- Purity
- lyophilized powder, suitable for cell culture
- Packaging
- 1mg
- Price
- $76.8
- Updated
- 2024/03/01
Elastase Chemical Properties,Usage,Production
Uses
Elastase is used in combination with other proteases for protein analysis by mass spectrometry. Elastase, is used in tissue dissociation because elastin is found in highest concentrations in the elastic fibers of connective tissues, elastase is frequently used to dissociate tissues that contain extensive intercellular fiber networks. It is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin, membrane protein solubilization, protein sequence studies.
Uses
Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
Uses
Elastase from porcine pancreas has been used:
- to treat vero cells?to study its effects on syncytium formation
- as a positive control in protease assays
- as a component in RPMI 1640 to isolate human aortic smooth muscle cells (HASMCs) from the aortic tissue
General Description
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Biochem/physiol Actions
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
Elastase Preparation Products And Raw materials
Raw materials
Preparation Products
Elastase Suppliers
View Lastest Price from Elastase manufacturers
- Product
- Elastase 39445-21-1
- Price
- US $100.00-25.00/kgkg
- Min. Order
- 1kgkg
- Purity
- 0.99
- Supply Ability
- 100
- Release date
- 2023-09-07
- Product
- Elastase 39445-21-1
- Price
- US $0.00-0.00/g
- Min. Order
- 1g
- Purity
- 98.0%
- Supply Ability
- 1kg/month
- Release date
- 2021-08-24
- Product
- Elastase 39445-21-1
- Price
- US $0.00-0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 500000kg
- Release date
- 2022-09-29