EC 3.1.4.4 TYPE IV Chemical Properties

storage temp. 

-70°C

solubility 

10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITONTM X-100: 1mg/mL

form 

lyophilized powder

color 

Light yellow to yellow

biological source

Streptomyces sp.

Specific Activity

≥60units/mg protein

Safety Information

Safety Statements 

7

WGK Germany 

3

F 

3-21

Storage Class

11 - Combustible Solids

Hazard Classifications

Resp. Sens. 1

MSDS

• Language:English Provider:SigmaAldrich

EC 3.1.4.4 TYPE IV Usage And Synthesis

Uses

Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

Uses

Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies.

General Description

Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.

Biochem/physiol Actions

Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

EC 3.1.4.4 TYPE IV(9001-87-0)Related Product Information

• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)
• Cupric acetylacetonate
• SALCOMINE
• Ferric acetylacetonate
• Ethyl isocyanoacetate
• METHYL ISOCYANOACETATE
• DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
• Tosylmethyl isocyanide
• 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
• TERT-BUTYL ISOCYANIDE
• COBALT(II) ACETYLACETONATE
• Aluminum acetylacetonate
• Benzyl isocyanide
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
• PHENYLSELENOL
• 2,4-PENTANEDIONE, SILVER DERIVATIVE
• Tris(2,4-pentanedionato)chroMiuM(III)
• N-BUTYLISOCYANIDE